Monitoring the growth effect of xenotransplanted human medulloblastoma in an immunocompromised mouse model using in vitro and ex vivo green fluorescent protein imaging

Shih Hwa Chiou, Chung Lan Kao, Han Tso Lin, Wen Ser Tseng, Ren Shyan Liu, Chen Fun Chung, Hung Hai Ku, C. P.Ching Po Lin, Tai-Tong Wong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction: Medulloblastoma (MB) is one of the most common malignant brain tumors in children. It is a radiosensitive tumor. At 5 years after radical surgical excision and craniospinal axis irradiation, the tumor-free survival rate is from 50 to 70% [Halperin EC, Constine LS, Tarbell NJ, Kun LE. Pediatric radiation oncology (2005)]. Case Report: In this study, we established xenotransplanted human MB (hMB) cells - Isochromosome 17q - In a severe combined immunodeficiency (SCID) mouse model. We further transduced green fluorescent protein (GFP) into hMB cells to evaluate these hMB cells grafted in SCID mice. Results: The result of an ex vivo GFP imaging system showed that a small lesion of the third-week-hMB-transplanted graft presented "green" signals with a clear tumor margin before any tumor-related symptoms were noted. We also demonstrated that the tumor progression could be monitored by GFP imaging for up to 12 weeks post-transplantation. Conclusions: This novel approach of GFP imaging assessment provides more accurate information of tumor status for experimental brain tumor studies. Because MB is sensitive to radiation and also response to chemotherapy, this SCID mouse model will be helpful for preclinical studies in the future.

Original languageEnglish
Pages (from-to)475-480
Number of pages6
JournalChild's Nervous System
Volume22
Issue number5
DOIs
Publication statusPublished - May 1 2006
Externally publishedYes

Fingerprint

Medulloblastoma
Green Fluorescent Proteins
Severe Combined Immunodeficiency
Growth
Neoplasms
Brain Neoplasms
Craniospinal Irradiation
Isochromosomes
Radiation Oncology
In Vitro Techniques
Transplantation
Radiation
Pediatrics
Transplants
Drug Therapy

Keywords

  • Green fluorescent protein imaging
  • Medulloblastoma
  • SCID mouse model

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Clinical Neurology

Cite this

Monitoring the growth effect of xenotransplanted human medulloblastoma in an immunocompromised mouse model using in vitro and ex vivo green fluorescent protein imaging. / Chiou, Shih Hwa; Kao, Chung Lan; Lin, Han Tso; Tseng, Wen Ser; Liu, Ren Shyan; Chung, Chen Fun; Ku, Hung Hai; Lin, C. P.Ching Po; Wong, Tai-Tong.

In: Child's Nervous System, Vol. 22, No. 5, 01.05.2006, p. 475-480.

Research output: Contribution to journalArticle

Chiou, Shih Hwa ; Kao, Chung Lan ; Lin, Han Tso ; Tseng, Wen Ser ; Liu, Ren Shyan ; Chung, Chen Fun ; Ku, Hung Hai ; Lin, C. P.Ching Po ; Wong, Tai-Tong. / Monitoring the growth effect of xenotransplanted human medulloblastoma in an immunocompromised mouse model using in vitro and ex vivo green fluorescent protein imaging. In: Child's Nervous System. 2006 ; Vol. 22, No. 5. pp. 475-480.
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AU - Tseng, Wen Ser

AU - Liu, Ren Shyan

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AU - Lin, C. P.Ching Po

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AB - Introduction: Medulloblastoma (MB) is one of the most common malignant brain tumors in children. It is a radiosensitive tumor. At 5 years after radical surgical excision and craniospinal axis irradiation, the tumor-free survival rate is from 50 to 70% [Halperin EC, Constine LS, Tarbell NJ, Kun LE. Pediatric radiation oncology (2005)]. Case Report: In this study, we established xenotransplanted human MB (hMB) cells - Isochromosome 17q - In a severe combined immunodeficiency (SCID) mouse model. We further transduced green fluorescent protein (GFP) into hMB cells to evaluate these hMB cells grafted in SCID mice. Results: The result of an ex vivo GFP imaging system showed that a small lesion of the third-week-hMB-transplanted graft presented "green" signals with a clear tumor margin before any tumor-related symptoms were noted. We also demonstrated that the tumor progression could be monitored by GFP imaging for up to 12 weeks post-transplantation. Conclusions: This novel approach of GFP imaging assessment provides more accurate information of tumor status for experimental brain tumor studies. Because MB is sensitive to radiation and also response to chemotherapy, this SCID mouse model will be helpful for preclinical studies in the future.

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