Abstract
To assess laser therapies in clinical practice, histologic examinations were commonly used. But histologic examinations were invasive and not real-time in nature. In this work, we validate multiphoton microscopy as a useful modality in evaluating laser-tissue reaction in vivo. Multiphoton microscopy based on femtosecond titanium-sapphire laser system were used to evaluate autoflurescence (AF) and second harmonic generation (SHG). Nude mouse skin was irradiated with Erbium:YAG laser at low to high fluence. High resolutional images can be obtained by multiphoton microscopy. At low fluence, Erbium:YAG laser can selectively loosen compact stratum corneum with minimal injury to basal layer. At high fluence, ablated keratinocytes and residual debris can be imaged. The laser thermal effect on dermis could be measured by SHG signals of collagen fibers. SHG decreased as laser fluence increased. Multiphoton microscopy is a useful in-vivo technique in evaluating ablative and thermal effects of Erbium:YAG laser on nude mouse skin.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 6842 |
| DOIs | |
| Publication status | Published - 2008 |
| Event | Photonic Therapeutics and Diagnostics IV - San Jose, CA, United States Duration: Jan 19 2008 → Jan 19 2008 |
Other
| Other | Photonic Therapeutics and Diagnostics IV |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/19/08 → 1/19/08 |
Fingerprint
Keywords
- Collagen
- Fluorescence
- Laser
- Multiphoton
- Second harmonic generation
ASJC Scopus subject areas
- Engineering(all)
Cite this
Monitoring laser-tissue interaction by non-linear optics. / Tsai, Tsung-Han; Lee, Jin Ning; Chan, Jung Yi; Hsu, Jr Ting; Tan, Hsin Yuan; Dong, Chen Yuan; Lee, Woan-Ruoh; Lin, Sung Jan.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6842 2008. 684202.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Monitoring laser-tissue interaction by non-linear optics
AU - Tsai, Tsung-Han
AU - Lee, Jin Ning
AU - Chan, Jung Yi
AU - Hsu, Jr Ting
AU - Tan, Hsin Yuan
AU - Dong, Chen Yuan
AU - Lee, Woan-Ruoh
AU - Lin, Sung Jan
PY - 2008
Y1 - 2008
N2 - To assess laser therapies in clinical practice, histologic examinations were commonly used. But histologic examinations were invasive and not real-time in nature. In this work, we validate multiphoton microscopy as a useful modality in evaluating laser-tissue reaction in vivo. Multiphoton microscopy based on femtosecond titanium-sapphire laser system were used to evaluate autoflurescence (AF) and second harmonic generation (SHG). Nude mouse skin was irradiated with Erbium:YAG laser at low to high fluence. High resolutional images can be obtained by multiphoton microscopy. At low fluence, Erbium:YAG laser can selectively loosen compact stratum corneum with minimal injury to basal layer. At high fluence, ablated keratinocytes and residual debris can be imaged. The laser thermal effect on dermis could be measured by SHG signals of collagen fibers. SHG decreased as laser fluence increased. Multiphoton microscopy is a useful in-vivo technique in evaluating ablative and thermal effects of Erbium:YAG laser on nude mouse skin.
AB - To assess laser therapies in clinical practice, histologic examinations were commonly used. But histologic examinations were invasive and not real-time in nature. In this work, we validate multiphoton microscopy as a useful modality in evaluating laser-tissue reaction in vivo. Multiphoton microscopy based on femtosecond titanium-sapphire laser system were used to evaluate autoflurescence (AF) and second harmonic generation (SHG). Nude mouse skin was irradiated with Erbium:YAG laser at low to high fluence. High resolutional images can be obtained by multiphoton microscopy. At low fluence, Erbium:YAG laser can selectively loosen compact stratum corneum with minimal injury to basal layer. At high fluence, ablated keratinocytes and residual debris can be imaged. The laser thermal effect on dermis could be measured by SHG signals of collagen fibers. SHG decreased as laser fluence increased. Multiphoton microscopy is a useful in-vivo technique in evaluating ablative and thermal effects of Erbium:YAG laser on nude mouse skin.
KW - Collagen
KW - Fluorescence
KW - Laser
KW - Multiphoton
KW - Second harmonic generation
UR - http://www.scopus.com/inward/record.url?scp=42149089076&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149089076&partnerID=8YFLogxK
U2 - 10.1117/12.761407
DO - 10.1117/12.761407
M3 - Conference contribution
SN - 9780819470171
VL - 6842
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -