Monitoring laser-tissue interaction by non-linear optics

Tsung-Han Tsai, Jin Ning Lee, Jung Yi Chan, Jr Ting Hsu, Hsin Yuan Tan, Chen Yuan Dong, Woan-Ruoh Lee, Sung Jan Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

To assess laser therapies in clinical practice, histologic examinations were commonly used. But histologic examinations were invasive and not real-time in nature. In this work, we validate multiphoton microscopy as a useful modality in evaluating laser-tissue reaction in vivo. Multiphoton microscopy based on femtosecond titanium-sapphire laser system were used to evaluate autoflurescence (AF) and second harmonic generation (SHG). Nude mouse skin was irradiated with Erbium:YAG laser at low to high fluence. High resolutional images can be obtained by multiphoton microscopy. At low fluence, Erbium:YAG laser can selectively loosen compact stratum corneum with minimal injury to basal layer. At high fluence, ablated keratinocytes and residual debris can be imaged. The laser thermal effect on dermis could be measured by SHG signals of collagen fibers. SHG decreased as laser fluence increased. Multiphoton microscopy is a useful in-vivo technique in evaluating ablative and thermal effects of Erbium:YAG laser on nude mouse skin.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6842
DOIs
Publication statusPublished - 2008
EventPhotonic Therapeutics and Diagnostics IV - San Jose, CA, United States
Duration: Jan 19 2008Jan 19 2008

Other

OtherPhotonic Therapeutics and Diagnostics IV
CountryUnited States
CitySan Jose, CA
Period1/19/081/19/08

Fingerprint

Laser tissue interaction
Nonlinear optics
Lasers
Monitoring
Microscopic examination
Erbium
Harmonic generation
Thermal effects
Skin
Collagen
Debris
Sapphire
Titanium
Tissue

Keywords

  • Collagen
  • Fluorescence
  • Laser
  • Multiphoton
  • Second harmonic generation

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Tsai, T-H., Lee, J. N., Chan, J. Y., Hsu, J. T., Tan, H. Y., Dong, C. Y., ... Lin, S. J. (2008). Monitoring laser-tissue interaction by non-linear optics. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6842). [684202] https://doi.org/10.1117/12.761407

Monitoring laser-tissue interaction by non-linear optics. / Tsai, Tsung-Han; Lee, Jin Ning; Chan, Jung Yi; Hsu, Jr Ting; Tan, Hsin Yuan; Dong, Chen Yuan; Lee, Woan-Ruoh; Lin, Sung Jan.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6842 2008. 684202.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsai, T-H, Lee, JN, Chan, JY, Hsu, JT, Tan, HY, Dong, CY, Lee, W-R & Lin, SJ 2008, Monitoring laser-tissue interaction by non-linear optics. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6842, 684202, Photonic Therapeutics and Diagnostics IV, San Jose, CA, United States, 1/19/08. https://doi.org/10.1117/12.761407
Tsai T-H, Lee JN, Chan JY, Hsu JT, Tan HY, Dong CY et al. Monitoring laser-tissue interaction by non-linear optics. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6842. 2008. 684202 https://doi.org/10.1117/12.761407
Tsai, Tsung-Han ; Lee, Jin Ning ; Chan, Jung Yi ; Hsu, Jr Ting ; Tan, Hsin Yuan ; Dong, Chen Yuan ; Lee, Woan-Ruoh ; Lin, Sung Jan. / Monitoring laser-tissue interaction by non-linear optics. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6842 2008.
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