Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy

Wen Lo, Chih Ting Hsu, Tsung-Rong Kuo, Chung Long Wu, Shu Jen Chiang, Sung Jan Lin, Shean Jen Chen, Chia Chun Chen, Chen Yuan Dong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Zinc oxide nanoparticles (ZnO NPs) are commonly used in sunscreens to reduce the risk of skin cancer by blocking ultraviolet radiation. ZnO NPs absorption through the transdermal route may not cause high health risk as inhalation or ingestion. However, in practical usage of sunscreens and cosmetics, ZnO NPs are topically applied to a large area of skin with long periods hence the potential absorption amount of ZnO NPs is still need to be concerned. Therefore, if the ZnO NPs are able the pass the barrier of normal skin, the pathways of transdermal delivery and the factors of enhancements become important issues. In this work, multiphoton microscopy provides us a non-invasive visualization of ZnO NPs in skin. Moreover, we quantitatively analyzed the enhancement of oleic acid and ethanol. Due to the fact that photoluminance of ZnO NPs spectrally overlaps autofluorence from skin stratum corneum (SC) and high turbidity of both ZnO NPs and SC, it is difficult to resolve the distribution of ZnO NPs in skin by using fluorescence microscopy. In this work, the second harmonic generation (SHG) signals from ZnO NPs which double the frequency of excitation source to characterize the delivery pathways and penetration depth in skin. Moreover, we quantitatively compare the ZnO NPs delivery efficiency in normal skin and in skins with three chemically enhancing conditions: ethanol, oleic acid and the combination of ethanol and oleic acid.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7548
DOIs
Publication statusPublished - 2010
Externally publishedYes
EventPhotonic Therapeutics and Diagnostics VI - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Other

OtherPhotonic Therapeutics and Diagnostics VI
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Fingerprint

Zinc Oxide
Zinc oxide
zinc oxides
Nanoparticles
Microscopy
delivery
Microscopic examination
Skin
microscopy
nanoparticles
Monitoring
oleic acid
Oleic acid
Oleic Acid
Sun hoods
Sunscreening Agents
Ethanol
ethyl alcohol
cornea
strata

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Lo, W., Hsu, C. T., Kuo, T-R., Wu, C. L., Chiang, S. J., Lin, S. J., ... Dong, C. Y. (2010). Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7548). [75480P] https://doi.org/10.1117/12.843187

Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy. / Lo, Wen; Hsu, Chih Ting; Kuo, Tsung-Rong; Wu, Chung Long; Chiang, Shu Jen; Lin, Sung Jan; Chen, Shean Jen; Chen, Chia Chun; Dong, Chen Yuan.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7548 2010. 75480P.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lo, W, Hsu, CT, Kuo, T-R, Wu, CL, Chiang, SJ, Lin, SJ, Chen, SJ, Chen, CC & Dong, CY 2010, Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7548, 75480P, Photonic Therapeutics and Diagnostics VI, San Francisco, CA, United States, 1/23/10. https://doi.org/10.1117/12.843187
Lo W, Hsu CT, Kuo T-R, Wu CL, Chiang SJ, Lin SJ et al. Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7548. 2010. 75480P https://doi.org/10.1117/12.843187
Lo, Wen ; Hsu, Chih Ting ; Kuo, Tsung-Rong ; Wu, Chung Long ; Chiang, Shu Jen ; Lin, Sung Jan ; Chen, Shean Jen ; Chen, Chia Chun ; Dong, Chen Yuan. / Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7548 2010.
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