Monitoring and quantitative assessment of tumor burden using in vivo bioluminescence imaging

Chia Chi Chen, Jeng Jong Hwang, Gann Ting, Yun Long Tseng, Shyh Jen Wang, Jaqueline Whang-Peng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In vivo bioluminescence imaging (BLI) is a sensitive imaging modality that is rapid and accessible, and may comprise an ideal tool for evaluating tumor growth. In this study, the kinetic of tumor growth has been assessed in C26 colon carcinoma bearing BALB/c mouse model. The ability of BLI to noninvasively quantitate the growth of subcutaneous tumors transplanted with C26 cells genetically engineered to stably express firefly luciferase and herpes simplex virus type-1 thymidine kinase (C26/tk-luc). A good correlation (R2=0.998) of photon emission to the cell number was found in vitro. Tumor burden and tumor volume were monitored in vivo over time by quantitation of photon emission using Xenogen IVIS 50 and standard external caliper measurement, respectively. At various time intervals, tumor-bearing mice were imaged to determine the correlation of in vivo BLI to tumor volume. However, a correlation of BLI to tumor volume was observed when tumor volume was smaller than 1000 mm3 (R2=0.907). γ Scintigraphy combined with [131I]FIAU was another imaging modality used for verifying the previous results. In conclusion, this study showed that bioluminescence imaging is a powerful and quantitative tool for the direct assay to monitor tumor growth in vivo. The dual reporter genes transfected tumor-bearing animal model can be applied in the evaluation of the efficacy of new developed anti-cancer drugs.

Original languageEnglish
Pages (from-to)437-441
Number of pages5
JournalNuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Volume571
Issue number1-2 SPEC. ISS.
DOIs
Publication statusPublished - Feb 1 2007
Externally publishedYes

Fingerprint

Bioluminescence
bioluminescence
Tumors
tumors
Imaging techniques
Monitoring
Bearings (structural)
mice
Photons
cancer
fireflies
thymidine
animal models
photons
viruses
cells
Viruses
genes
Assays
Animals

Keywords

  • [131I]FIAU
  • Bioluminescence imaging
  • Firefly luciferase
  • Gamma scintigraphy
  • Mouse syngenic model
  • Murine colon carcinoma
  • Xenogen IVIS 50

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

Cite this

Monitoring and quantitative assessment of tumor burden using in vivo bioluminescence imaging. / Chen, Chia Chi; Hwang, Jeng Jong; Ting, Gann; Tseng, Yun Long; Wang, Shyh Jen; Whang-Peng, Jaqueline.

In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 571, No. 1-2 SPEC. ISS., 01.02.2007, p. 437-441.

Research output: Contribution to journalArticle

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