Molecular structure of (m5 dC-dG)3: The role of the methyl group on 5-methyl cytosine in stabilizing Z-DNA

Satoshi Fujii, Andrew H.J. Wang, Gijs van der Marel, Jacques H. van Boom, Alexander Rich

Research output: Contribution to journalArticlepeer-review

153 Citations (Scopus)

Abstract

The hexamer (m5dC-dG)3 has been synthesized and its three-dimensional structure determined by a single crystal X-ray diffraction analysis. The structure has been refined to a final R valne of 15.6% at 1.3 Å resolution. The molecule forms a left-handed Z-DNA helix which is similar to the unmethylated Z-DNA structure. The presence of the methyl group has resulted in slight changes in the twist angle between snccessive base pairs and modification of some of the interatomic contacts. Methylation of cytosine in the C5 position is associated with a relative destabilization of the B-DNA structure and a stabilization through hydrophobic bonding of the Z-DNA structure.

Original languageEnglish
Pages (from-to)7879-7892
Number of pages14
JournalNucleic Acids Research
Volume10
Issue number23
DOIs
Publication statusPublished - Dec 11 1982
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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