Molecular screening of multidrug-resistance tuberculosis by a designated public health laboratory in Taiwan

H. C. Lin, C. L. Perng, Y. W. Lai, F. G. Lin, C. J. Chiang, H. A. Lin, R. Jou, T. S. Chiueh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This manuscript describes our experience in early identifying MDR-TB cases in high-risk populations by setting up a single-referral molecular diagnosis laboratory in Taiwan. Taiwan Centers for Disease Control designated a single-referral laboratory to provide the GenoType MTBDRplus test for screening high-risk MDR-TB populations nationwide in 2012–2015. A total of 5,838 sputum specimens from 3,308 patients were tested within 3 days turnaround time. Compared with the conventional culture and drug susceptibility testing, the overall performance of the GenoType MTBDRplus test for detecting TB infection showed accuracy of 70.7%, sensitivity of 85.9%, specificity of 65.7%, positive predictive value of 45.5%, and negative predictive value of 93.3%. And the accuracy of detecting rifampin (RIF) resistance, isoniazid (INH) resistance, and MDR-TB (resistant to at least RIF and INH) were 96.5%, 95.2%, and 97.7%, respectively. MDR-TB contacts presented a higher rate of mutated codons 513–519, GenoType MTBDRplus banding pattern: rpoB WT3(−), and rpoB WT4(−) than the treatment failure group. The MDR-TB contact group also had a higher rate of inhA C15T mutation, banding pattern: inhA WT1(−), and inhA MUT1(+) than the recurrent group. Resistance profiles of MDR-TB isolates also varied geographically. The referral molecular diagnosis system contributed to rapid detection and initiation of appropriate therapy.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
DOIs
Publication statusAccepted/In press - Aug 24 2017
Externally publishedYes

Fingerprint

Multiple Drug Resistance
Taiwan
Tuberculosis
Referral and Consultation
Public Health
Genotype
Rifampin
Clinical Laboratory Techniques
Isoniazid
Centers for Disease Control and Prevention (U.S.)
Sputum
Treatment Failure
Codon
Population
Sensitivity and Specificity
Mutation
Infection
Pharmaceutical Preparations
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Molecular screening of multidrug-resistance tuberculosis by a designated public health laboratory in Taiwan. / Lin, H. C.; Perng, C. L.; Lai, Y. W.; Lin, F. G.; Chiang, C. J.; Lin, H. A.; Jou, R.; Chiueh, T. S.

In: European Journal of Clinical Microbiology and Infectious Diseases, 24.08.2017, p. 1-9.

Research output: Contribution to journalArticle

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