Molecular Mechanisms Underlying Yatein-Induced Cell-Cycle Arrest and Microtubule Destabilization in Human Lung Adenocarcinoma Cells

Shang-Tse Ho, Chi-Chen Lin, Yu-Tang Tung, Jyh-Horng Wu

Research output: Contribution to journalArticle

Abstract

Yatein is an antitumor agent isolated from Calocedrus formosana Florin leaves extract. In our previous study, we found that yatein inhibited the growth of human lung adenocarcinoma A549 and CL1-5 cells by inducing intrinsic and extrinsic apoptotic pathways. To further uncover the effects and mechanisms of yatein-induced inhibition on A549 and CL1-5 cell growth, we evaluated yatein-mediated antitumor activity in vivo and the regulatory effects of yatein on cell-cycle progression and microtubule dynamics. Flow cytometry and western blotting revealed that yatein induces G2/M arrest in A549 and CL1-5 cells. Yatein also destabilized microtubules and interfered with microtubule dynamics in the two cell lines. Furthermore, we evaluated the antitumor activity of yatein in vivo using a xenograft mouse model and found that yatein treatment altered cyclin B/Cdc2 complex expression and significantly inhibited tumor growth. Taken together, our results suggested that yatein effectively inhibited the growth of A549 and CL1-5 cells possibly by disrupting cell-cycle progression and microtubule dynamics.

Original languageEnglish
JournalCancers
Volume11
Issue number9
DOIs
Publication statusPublished - Sep 17 2019

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Cell Cycle Checkpoints
Microtubules
Growth
Cell Cycle
Cupressaceae
yatein
Adenocarcinoma of lung
Cyclin B
Heterografts
Antineoplastic Agents
Flow Cytometry
Western Blotting
Cell Line

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Molecular Mechanisms Underlying Yatein-Induced Cell-Cycle Arrest and Microtubule Destabilization in Human Lung Adenocarcinoma Cells. / Ho, Shang-Tse; Lin, Chi-Chen; Tung, Yu-Tang; Wu, Jyh-Horng.

In: Cancers, Vol. 11, No. 9, 17.09.2019.

Research output: Contribution to journalArticle

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