Molecular interactions between poly(ADP-ribose) polymerase (PARP I) and topoisomerase I (Topo I): Identification of topology of binding

Pal I. Bauer, Hui Je Chen, Erzsebet Kenesi, Istvan Kenessey, Kalman G. Buki, Eva Kirsten, Alaeddin Hakam, Jaulang I. Hwang, Ernest Kun

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The molecular interactions of poly(ADP-ribose) polymerase I (PARP I) and topoisomerase I (Topo I) have been determined by the analysis of physical binding of the two proteins and some of their polypeptide components and by the effect of PARP I on the enzymatic catalysis of Topo I. Direct association of Topo I and PARP I as well as the binding of two Topo I polypeptides to PARP I are demonstrated. The effect of PARP I on the 'global' Topo I reaction (scission and religation), and the activation of Topo I by the 36 kDa polypeptide of PARP I and catalytic modifications by poly(ADP-ribosyl)ation are also shown. The covalent binding of Topo I to circular DNA is activated by PARP I similar to the degree of activation of the 'global' Topo I reaction, whereas the religation of DNA is unaffected by PARP I. The geometry of PARP I-Topo I interaction compared to automodified PARP I was reconstructed from direct binding assays between glutathione S-transferase fusion polypeptides of Topo I and PARP I demonstrating highly selective binding, which was correlated with amino acid sequences and with the 'C clamp' model derived from X-ray crystallography.

Original languageEnglish
Pages (from-to)239-242
Number of pages4
JournalFEBS Letters
Volume506
Issue number3
DOIs
Publication statusPublished - Oct 12 2001

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Type I DNA Topoisomerase
Molecular interactions
Poly(ADP-ribose) Polymerases
Topology
Peptides
Chemical activation
Circular DNA
X ray crystallography
X Ray Crystallography
Clamping devices
Glutathione Transferase
Catalysis
Adenosine Diphosphate
Amino Acid Sequence
Assays
Carrier Proteins
Fusion reactions
Association reactions
Amino Acids

Keywords

  • Binding site
  • Poly(ADP-ribose) polymerase I
  • Topoisomerase I binding
  • Topoisomerase I regulation by poly(ADP-ribose) polymerase I
  • Topology interaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular interactions between poly(ADP-ribose) polymerase (PARP I) and topoisomerase I (Topo I) : Identification of topology of binding. / Bauer, Pal I.; Chen, Hui Je; Kenesi, Erzsebet; Kenessey, Istvan; Buki, Kalman G.; Kirsten, Eva; Hakam, Alaeddin; Hwang, Jaulang I.; Kun, Ernest.

In: FEBS Letters, Vol. 506, No. 3, 12.10.2001, p. 239-242.

Research output: Contribution to journalArticle

Bauer, PI, Chen, HJ, Kenesi, E, Kenessey, I, Buki, KG, Kirsten, E, Hakam, A, Hwang, JI & Kun, E 2001, 'Molecular interactions between poly(ADP-ribose) polymerase (PARP I) and topoisomerase I (Topo I): Identification of topology of binding', FEBS Letters, vol. 506, no. 3, pp. 239-242. https://doi.org/10.1016/S0014-5793(01)02919-2
Bauer, Pal I. ; Chen, Hui Je ; Kenesi, Erzsebet ; Kenessey, Istvan ; Buki, Kalman G. ; Kirsten, Eva ; Hakam, Alaeddin ; Hwang, Jaulang I. ; Kun, Ernest. / Molecular interactions between poly(ADP-ribose) polymerase (PARP I) and topoisomerase I (Topo I) : Identification of topology of binding. In: FEBS Letters. 2001 ; Vol. 506, No. 3. pp. 239-242.
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