Molecular imaging of enhanced Na+ expression in the liver of total sleep deprived rats by TOF-SIMS

Hung Ming Chang, Bo Jung Chen, Un In Wu, Yi Lun Huang, Fu Der Mai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sleep disorder is associated with metabolic disturbances, which was related to oxidative stress and subsequently sodium overload. Since liver plays important roles in metabolic regulation, present study is aimed to determine whether hepatic sodium, together with oxidative stress, would significantly alter after total sleep deprivation (TSD). Sodium ion was investigated by time-of-flight secondary ion mass spectrometry (TOF-SIMS). Parameter for oxidative stress was examined by heat shock protein-25 (HSP-25) immunohistochemistry. TOF-SIMS spectrum indicated that hepatic Na+/K+ ratio counting as 82.41 ± 9.5 was obtained in normal rats. Sodium ions were distributed in hepatocytes with several aggregations. However, following TSD, the intensity for Na+/K+ ratio was relatively increased (101.94 ± 6.9) and signals for sodium image were strongly expressed throughout hepatocytes without spatial localization. Quantitative analysis revealed that HSP-25 staining intensity is 1.78 ± 0.27 in TSD rats, which was significantly higher than that of normal ones (0.68 ± 0.15). HSP-25 augmentation suggests that hepatocytes suffer from oxidative stress following TSD. Concerning oxidative stress induced sodium overload would impair metabolic function; enhanced hepatic sodium expression after TSD may be a major cause of TSD relevant metabolic diseases.

Original languageEnglish
Pages (from-to)1131-1134
Number of pages4
JournalApplied Surface Science
Volume255
Issue number4
DOIs
Publication statusPublished - Dec 15 2008

Fingerprint

Molecular imaging
Secondary ion mass spectrometry
Liver
Rats
Oxidative stress
Sodium
Heat-Shock Proteins
Proteins
Ions
Sleep
Agglomeration
Chemical analysis

Keywords

  • Hepatocyte
  • Image analysis
  • Oxidative stress
  • Sleep deprivation
  • Sodium
  • TOF-SIMS

ASJC Scopus subject areas

  • Surfaces, Coatings and Films

Cite this

Molecular imaging of enhanced Na+ expression in the liver of total sleep deprived rats by TOF-SIMS. / Chang, Hung Ming; Chen, Bo Jung; Wu, Un In; Huang, Yi Lun; Mai, Fu Der.

In: Applied Surface Science, Vol. 255, No. 4, 15.12.2008, p. 1131-1134.

Research output: Contribution to journalArticle

@article{832404a5420f498da8d41b3852633f57,
title = "Molecular imaging of enhanced Na+ expression in the liver of total sleep deprived rats by TOF-SIMS",
abstract = "Sleep disorder is associated with metabolic disturbances, which was related to oxidative stress and subsequently sodium overload. Since liver plays important roles in metabolic regulation, present study is aimed to determine whether hepatic sodium, together with oxidative stress, would significantly alter after total sleep deprivation (TSD). Sodium ion was investigated by time-of-flight secondary ion mass spectrometry (TOF-SIMS). Parameter for oxidative stress was examined by heat shock protein-25 (HSP-25) immunohistochemistry. TOF-SIMS spectrum indicated that hepatic Na+/K+ ratio counting as 82.41 ± 9.5 was obtained in normal rats. Sodium ions were distributed in hepatocytes with several aggregations. However, following TSD, the intensity for Na+/K+ ratio was relatively increased (101.94 ± 6.9) and signals for sodium image were strongly expressed throughout hepatocytes without spatial localization. Quantitative analysis revealed that HSP-25 staining intensity is 1.78 ± 0.27 in TSD rats, which was significantly higher than that of normal ones (0.68 ± 0.15). HSP-25 augmentation suggests that hepatocytes suffer from oxidative stress following TSD. Concerning oxidative stress induced sodium overload would impair metabolic function; enhanced hepatic sodium expression after TSD may be a major cause of TSD relevant metabolic diseases.",
keywords = "Hepatocyte, Image analysis, Oxidative stress, Sleep deprivation, Sodium, TOF-SIMS",
author = "Chang, {Hung Ming} and Chen, {Bo Jung} and Wu, {Un In} and Huang, {Yi Lun} and Mai, {Fu Der}",
year = "2008",
month = "12",
day = "15",
doi = "10.1016/j.apsusc.2008.05.147",
language = "English",
volume = "255",
pages = "1131--1134",
journal = "Applied Surface Science",
issn = "0169-4332",
publisher = "Elsevier B.V.",
number = "4",

}

TY - JOUR

T1 - Molecular imaging of enhanced Na+ expression in the liver of total sleep deprived rats by TOF-SIMS

AU - Chang, Hung Ming

AU - Chen, Bo Jung

AU - Wu, Un In

AU - Huang, Yi Lun

AU - Mai, Fu Der

PY - 2008/12/15

Y1 - 2008/12/15

N2 - Sleep disorder is associated with metabolic disturbances, which was related to oxidative stress and subsequently sodium overload. Since liver plays important roles in metabolic regulation, present study is aimed to determine whether hepatic sodium, together with oxidative stress, would significantly alter after total sleep deprivation (TSD). Sodium ion was investigated by time-of-flight secondary ion mass spectrometry (TOF-SIMS). Parameter for oxidative stress was examined by heat shock protein-25 (HSP-25) immunohistochemistry. TOF-SIMS spectrum indicated that hepatic Na+/K+ ratio counting as 82.41 ± 9.5 was obtained in normal rats. Sodium ions were distributed in hepatocytes with several aggregations. However, following TSD, the intensity for Na+/K+ ratio was relatively increased (101.94 ± 6.9) and signals for sodium image were strongly expressed throughout hepatocytes without spatial localization. Quantitative analysis revealed that HSP-25 staining intensity is 1.78 ± 0.27 in TSD rats, which was significantly higher than that of normal ones (0.68 ± 0.15). HSP-25 augmentation suggests that hepatocytes suffer from oxidative stress following TSD. Concerning oxidative stress induced sodium overload would impair metabolic function; enhanced hepatic sodium expression after TSD may be a major cause of TSD relevant metabolic diseases.

AB - Sleep disorder is associated with metabolic disturbances, which was related to oxidative stress and subsequently sodium overload. Since liver plays important roles in metabolic regulation, present study is aimed to determine whether hepatic sodium, together with oxidative stress, would significantly alter after total sleep deprivation (TSD). Sodium ion was investigated by time-of-flight secondary ion mass spectrometry (TOF-SIMS). Parameter for oxidative stress was examined by heat shock protein-25 (HSP-25) immunohistochemistry. TOF-SIMS spectrum indicated that hepatic Na+/K+ ratio counting as 82.41 ± 9.5 was obtained in normal rats. Sodium ions were distributed in hepatocytes with several aggregations. However, following TSD, the intensity for Na+/K+ ratio was relatively increased (101.94 ± 6.9) and signals for sodium image were strongly expressed throughout hepatocytes without spatial localization. Quantitative analysis revealed that HSP-25 staining intensity is 1.78 ± 0.27 in TSD rats, which was significantly higher than that of normal ones (0.68 ± 0.15). HSP-25 augmentation suggests that hepatocytes suffer from oxidative stress following TSD. Concerning oxidative stress induced sodium overload would impair metabolic function; enhanced hepatic sodium expression after TSD may be a major cause of TSD relevant metabolic diseases.

KW - Hepatocyte

KW - Image analysis

KW - Oxidative stress

KW - Sleep deprivation

KW - Sodium

KW - TOF-SIMS

UR - http://www.scopus.com/inward/record.url?scp=56449090767&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56449090767&partnerID=8YFLogxK

U2 - 10.1016/j.apsusc.2008.05.147

DO - 10.1016/j.apsusc.2008.05.147

M3 - Article

AN - SCOPUS:56449090767

VL - 255

SP - 1131

EP - 1134

JO - Applied Surface Science

JF - Applied Surface Science

SN - 0169-4332

IS - 4

ER -