Molecular epidemiology of HIV-1 subtypes and drug resistant strains in Taiwan

Yu Ching Lan, Tarek Elbeik, Jo Ann Dileanis, Valerie Ng, Yen Ju Chen, Hsieh Shong Leu, Shu Hsing Cheng, Jen Chien Wong, Wing Wai Wong, Yi Ming A. Chen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The Taiwanese government has provided free highly active antiretroviral therapy since April 1997. Previously, we have reported on the molecular epidemiology of HIV-1 in Taiwan from 1988 to 1998. In addition, an outbreak of circulating recombinant form (CRF) 07_BC among intravenous drug users was noted in 2004. Therefore, the purposes of this study were to elucidate the distribution of HIV-1 subtypes among different high-risk groups in Taiwan from 1999 to 2000 and to conduct surveillance on drug resistance among treatment naive patients from 1997 to 2000. Blood samples from 239 HIV-1/AIDS patients and their subtypes were examined using DNA sequencing and phylogenetic analysis. The results showed that among 226 male patients, 213 (94.2%) had subtype B, 11 (4.9%) had CRF01_AE, 1 had unique recombinant strain related to both CRF07_BC and CRF08_BC (strain T12-99TW) and 1 had CRF08_BC (strain L9312-00TW). The patients infected with T12-99TW and L9312-00TW were intravenous drug users and had needle-sharing experiences in Yunnan Province, China. Of the 13 HIV-1-infected females, 7 (53.8%) had subtype B, 5 (38.5%) had CRF01_AE, and 1 (7.7%) had subtype C. Phylogenetic analysis demonstrated that the neither strain T12-99TW nor L9312-00TW clustered with CRF07_BC strains isolated from Taiwanese intravenous drug users in 2004. In addition, 126 treatment naive patients were selected for genotypic DR analysis and the results showed that 4.3% (2/47) homosexual males had M184V mutations. This is the first report on the identification of CRF08_BC and a unique recombinant strain related to both CRF07_BC and CRF08_BC in Taiwan.

Original languageEnglish
Pages (from-to)183-191
Number of pages9
JournalJournal of Medical Virology
Volume80
Issue number2
DOIs
Publication statusPublished - Feb 1 2008
Externally publishedYes

Fingerprint

Molecular Epidemiology
Taiwan
HIV-1
Drug Users
Pharmaceutical Preparations
Needle Sharing
Highly Active Antiretroviral Therapy
DNA Sequence Analysis
Drug Resistance
Disease Outbreaks
China
Acquired Immunodeficiency Syndrome
Mutation
Therapeutics

Keywords

  • HIV drug resistance
  • HIV subtype
  • Molecular epidemiology

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Molecular epidemiology of HIV-1 subtypes and drug resistant strains in Taiwan. / Lan, Yu Ching; Elbeik, Tarek; Dileanis, Jo Ann; Ng, Valerie; Chen, Yen Ju; Leu, Hsieh Shong; Cheng, Shu Hsing; Wong, Jen Chien; Wong, Wing Wai; Chen, Yi Ming A.

In: Journal of Medical Virology, Vol. 80, No. 2, 01.02.2008, p. 183-191.

Research output: Contribution to journalArticle

Lan, YC, Elbeik, T, Dileanis, JA, Ng, V, Chen, YJ, Leu, HS, Cheng, SH, Wong, JC, Wong, WW & Chen, YMA 2008, 'Molecular epidemiology of HIV-1 subtypes and drug resistant strains in Taiwan', Journal of Medical Virology, vol. 80, no. 2, pp. 183-191. https://doi.org/10.1002/jmv.21065
Lan, Yu Ching ; Elbeik, Tarek ; Dileanis, Jo Ann ; Ng, Valerie ; Chen, Yen Ju ; Leu, Hsieh Shong ; Cheng, Shu Hsing ; Wong, Jen Chien ; Wong, Wing Wai ; Chen, Yi Ming A. / Molecular epidemiology of HIV-1 subtypes and drug resistant strains in Taiwan. In: Journal of Medical Virology. 2008 ; Vol. 80, No. 2. pp. 183-191.
@article{1f7dff8869c54251ae86a0d6bebf981d,
title = "Molecular epidemiology of HIV-1 subtypes and drug resistant strains in Taiwan",
abstract = "The Taiwanese government has provided free highly active antiretroviral therapy since April 1997. Previously, we have reported on the molecular epidemiology of HIV-1 in Taiwan from 1988 to 1998. In addition, an outbreak of circulating recombinant form (CRF) 07_BC among intravenous drug users was noted in 2004. Therefore, the purposes of this study were to elucidate the distribution of HIV-1 subtypes among different high-risk groups in Taiwan from 1999 to 2000 and to conduct surveillance on drug resistance among treatment naive patients from 1997 to 2000. Blood samples from 239 HIV-1/AIDS patients and their subtypes were examined using DNA sequencing and phylogenetic analysis. The results showed that among 226 male patients, 213 (94.2{\%}) had subtype B, 11 (4.9{\%}) had CRF01_AE, 1 had unique recombinant strain related to both CRF07_BC and CRF08_BC (strain T12-99TW) and 1 had CRF08_BC (strain L9312-00TW). The patients infected with T12-99TW and L9312-00TW were intravenous drug users and had needle-sharing experiences in Yunnan Province, China. Of the 13 HIV-1-infected females, 7 (53.8{\%}) had subtype B, 5 (38.5{\%}) had CRF01_AE, and 1 (7.7{\%}) had subtype C. Phylogenetic analysis demonstrated that the neither strain T12-99TW nor L9312-00TW clustered with CRF07_BC strains isolated from Taiwanese intravenous drug users in 2004. In addition, 126 treatment naive patients were selected for genotypic DR analysis and the results showed that 4.3{\%} (2/47) homosexual males had M184V mutations. This is the first report on the identification of CRF08_BC and a unique recombinant strain related to both CRF07_BC and CRF08_BC in Taiwan.",
keywords = "HIV drug resistance, HIV subtype, Molecular epidemiology",
author = "Lan, {Yu Ching} and Tarek Elbeik and Dileanis, {Jo Ann} and Valerie Ng and Chen, {Yen Ju} and Leu, {Hsieh Shong} and Cheng, {Shu Hsing} and Wong, {Jen Chien} and Wong, {Wing Wai} and Chen, {Yi Ming A.}",
year = "2008",
month = "2",
day = "1",
doi = "10.1002/jmv.21065",
language = "English",
volume = "80",
pages = "183--191",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Molecular epidemiology of HIV-1 subtypes and drug resistant strains in Taiwan

AU - Lan, Yu Ching

AU - Elbeik, Tarek

AU - Dileanis, Jo Ann

AU - Ng, Valerie

AU - Chen, Yen Ju

AU - Leu, Hsieh Shong

AU - Cheng, Shu Hsing

AU - Wong, Jen Chien

AU - Wong, Wing Wai

AU - Chen, Yi Ming A.

PY - 2008/2/1

Y1 - 2008/2/1

N2 - The Taiwanese government has provided free highly active antiretroviral therapy since April 1997. Previously, we have reported on the molecular epidemiology of HIV-1 in Taiwan from 1988 to 1998. In addition, an outbreak of circulating recombinant form (CRF) 07_BC among intravenous drug users was noted in 2004. Therefore, the purposes of this study were to elucidate the distribution of HIV-1 subtypes among different high-risk groups in Taiwan from 1999 to 2000 and to conduct surveillance on drug resistance among treatment naive patients from 1997 to 2000. Blood samples from 239 HIV-1/AIDS patients and their subtypes were examined using DNA sequencing and phylogenetic analysis. The results showed that among 226 male patients, 213 (94.2%) had subtype B, 11 (4.9%) had CRF01_AE, 1 had unique recombinant strain related to both CRF07_BC and CRF08_BC (strain T12-99TW) and 1 had CRF08_BC (strain L9312-00TW). The patients infected with T12-99TW and L9312-00TW were intravenous drug users and had needle-sharing experiences in Yunnan Province, China. Of the 13 HIV-1-infected females, 7 (53.8%) had subtype B, 5 (38.5%) had CRF01_AE, and 1 (7.7%) had subtype C. Phylogenetic analysis demonstrated that the neither strain T12-99TW nor L9312-00TW clustered with CRF07_BC strains isolated from Taiwanese intravenous drug users in 2004. In addition, 126 treatment naive patients were selected for genotypic DR analysis and the results showed that 4.3% (2/47) homosexual males had M184V mutations. This is the first report on the identification of CRF08_BC and a unique recombinant strain related to both CRF07_BC and CRF08_BC in Taiwan.

AB - The Taiwanese government has provided free highly active antiretroviral therapy since April 1997. Previously, we have reported on the molecular epidemiology of HIV-1 in Taiwan from 1988 to 1998. In addition, an outbreak of circulating recombinant form (CRF) 07_BC among intravenous drug users was noted in 2004. Therefore, the purposes of this study were to elucidate the distribution of HIV-1 subtypes among different high-risk groups in Taiwan from 1999 to 2000 and to conduct surveillance on drug resistance among treatment naive patients from 1997 to 2000. Blood samples from 239 HIV-1/AIDS patients and their subtypes were examined using DNA sequencing and phylogenetic analysis. The results showed that among 226 male patients, 213 (94.2%) had subtype B, 11 (4.9%) had CRF01_AE, 1 had unique recombinant strain related to both CRF07_BC and CRF08_BC (strain T12-99TW) and 1 had CRF08_BC (strain L9312-00TW). The patients infected with T12-99TW and L9312-00TW were intravenous drug users and had needle-sharing experiences in Yunnan Province, China. Of the 13 HIV-1-infected females, 7 (53.8%) had subtype B, 5 (38.5%) had CRF01_AE, and 1 (7.7%) had subtype C. Phylogenetic analysis demonstrated that the neither strain T12-99TW nor L9312-00TW clustered with CRF07_BC strains isolated from Taiwanese intravenous drug users in 2004. In addition, 126 treatment naive patients were selected for genotypic DR analysis and the results showed that 4.3% (2/47) homosexual males had M184V mutations. This is the first report on the identification of CRF08_BC and a unique recombinant strain related to both CRF07_BC and CRF08_BC in Taiwan.

KW - HIV drug resistance

KW - HIV subtype

KW - Molecular epidemiology

UR - http://www.scopus.com/inward/record.url?scp=37548999033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37548999033&partnerID=8YFLogxK

U2 - 10.1002/jmv.21065

DO - 10.1002/jmv.21065

M3 - Article

VL - 80

SP - 183

EP - 191

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 2

ER -