Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan

Yu Ting Lin, Yu Ching Lan, Yen Ju Chen, Yu Hui Huang, Cheng Ming Lee, Tze Tze Liu, Wing Wai Wong, Jyh Yuan Yang, Chin Tien Wang, Yi Ming Arthur Chen

Research output: Contribution to journalArticle

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Abstract

Background. Previously, we reported that there was an outbreak of human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The objectives of the present study were to conduct a molecular epidemiological analysis and to characterize the full-length genome of the Taiwanese CRF07_BC. Methods. Three hundred and fifty-eight patients with HIV-1/AIDS from hospitals and 133 HIV-1-infected inmates from detention centers were recruited. DNA sequencing and phylogenetic analysis were conducted to determine subtypes and evolutionary relationships. Recombination breakpoints of 2 full-length CRF07_BC strains were elucidated using a bootscanning method. Results. Of 206 HIV-1-infected patients who received a diagnosis in 2004, 44.7% were infected with subtype B, 53.4% with CRF07_BC, and 1.5% with CRF01_AE. Ninety-eight percent (109/111) of IDUs were infected with CRF07_BC. Deletions of 7-11 amino acids in both p6gag and p6pol proteins were noted among the Taiwanese CRF07_BC strains. The CRF_07BC strains belonged to 2 phylogenetic clusters, and the first cluster contained only CRF07_BC strains from the southern part of Taiwan. Conclusions. The Taiwanese CRF07_BC strains had 97% full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004.

Original languageEnglish
Pages (from-to)1283-1293
Number of pages11
JournalJournal of Infectious Diseases
Volume195
Issue number9
DOIs
Publication statusPublished - May 1 2007
Externally publishedYes

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Molecular Epidemiology
Virus Diseases
Drug Users
Taiwan
HIV-1
Injections
Sequence Homology
DNA Sequence Analysis
Genetic Recombination
Disease Outbreaks
China
Acquired Immunodeficiency Syndrome
Genome
Amino Acids
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan. / Lin, Yu Ting; Lan, Yu Ching; Chen, Yen Ju; Huang, Yu Hui; Lee, Cheng Ming; Liu, Tze Tze; Wong, Wing Wai; Yang, Jyh Yuan; Wang, Chin Tien; Chen, Yi Ming Arthur.

In: Journal of Infectious Diseases, Vol. 195, No. 9, 01.05.2007, p. 1283-1293.

Research output: Contribution to journalArticle

Lin, Yu Ting ; Lan, Yu Ching ; Chen, Yen Ju ; Huang, Yu Hui ; Lee, Cheng Ming ; Liu, Tze Tze ; Wong, Wing Wai ; Yang, Jyh Yuan ; Wang, Chin Tien ; Chen, Yi Ming Arthur. / Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan. In: Journal of Infectious Diseases. 2007 ; Vol. 195, No. 9. pp. 1283-1293.
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abstract = "Background. Previously, we reported that there was an outbreak of human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The objectives of the present study were to conduct a molecular epidemiological analysis and to characterize the full-length genome of the Taiwanese CRF07_BC. Methods. Three hundred and fifty-eight patients with HIV-1/AIDS from hospitals and 133 HIV-1-infected inmates from detention centers were recruited. DNA sequencing and phylogenetic analysis were conducted to determine subtypes and evolutionary relationships. Recombination breakpoints of 2 full-length CRF07_BC strains were elucidated using a bootscanning method. Results. Of 206 HIV-1-infected patients who received a diagnosis in 2004, 44.7{\%} were infected with subtype B, 53.4{\%} with CRF07_BC, and 1.5{\%} with CRF01_AE. Ninety-eight percent (109/111) of IDUs were infected with CRF07_BC. Deletions of 7-11 amino acids in both p6gag and p6pol proteins were noted among the Taiwanese CRF07_BC strains. The CRF_07BC strains belonged to 2 phylogenetic clusters, and the first cluster contained only CRF07_BC strains from the southern part of Taiwan. Conclusions. The Taiwanese CRF07_BC strains had 97{\%} full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004.",
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AU - Lin, Yu Ting

AU - Lan, Yu Ching

AU - Chen, Yen Ju

AU - Huang, Yu Hui

AU - Lee, Cheng Ming

AU - Liu, Tze Tze

AU - Wong, Wing Wai

AU - Yang, Jyh Yuan

AU - Wang, Chin Tien

AU - Chen, Yi Ming Arthur

PY - 2007/5/1

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N2 - Background. Previously, we reported that there was an outbreak of human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The objectives of the present study were to conduct a molecular epidemiological analysis and to characterize the full-length genome of the Taiwanese CRF07_BC. Methods. Three hundred and fifty-eight patients with HIV-1/AIDS from hospitals and 133 HIV-1-infected inmates from detention centers were recruited. DNA sequencing and phylogenetic analysis were conducted to determine subtypes and evolutionary relationships. Recombination breakpoints of 2 full-length CRF07_BC strains were elucidated using a bootscanning method. Results. Of 206 HIV-1-infected patients who received a diagnosis in 2004, 44.7% were infected with subtype B, 53.4% with CRF07_BC, and 1.5% with CRF01_AE. Ninety-eight percent (109/111) of IDUs were infected with CRF07_BC. Deletions of 7-11 amino acids in both p6gag and p6pol proteins were noted among the Taiwanese CRF07_BC strains. The CRF_07BC strains belonged to 2 phylogenetic clusters, and the first cluster contained only CRF07_BC strains from the southern part of Taiwan. Conclusions. The Taiwanese CRF07_BC strains had 97% full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004.

AB - Background. Previously, we reported that there was an outbreak of human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The objectives of the present study were to conduct a molecular epidemiological analysis and to characterize the full-length genome of the Taiwanese CRF07_BC. Methods. Three hundred and fifty-eight patients with HIV-1/AIDS from hospitals and 133 HIV-1-infected inmates from detention centers were recruited. DNA sequencing and phylogenetic analysis were conducted to determine subtypes and evolutionary relationships. Recombination breakpoints of 2 full-length CRF07_BC strains were elucidated using a bootscanning method. Results. Of 206 HIV-1-infected patients who received a diagnosis in 2004, 44.7% were infected with subtype B, 53.4% with CRF07_BC, and 1.5% with CRF01_AE. Ninety-eight percent (109/111) of IDUs were infected with CRF07_BC. Deletions of 7-11 amino acids in both p6gag and p6pol proteins were noted among the Taiwanese CRF07_BC strains. The CRF_07BC strains belonged to 2 phylogenetic clusters, and the first cluster contained only CRF07_BC strains from the southern part of Taiwan. Conclusions. The Taiwanese CRF07_BC strains had 97% full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004.

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