Molecular epidemiology of emerging blaOXA-23-Like- and blaOXA-24-Like-carrying acinetobacter baumannii in Taiwan

Shu Chen Kuo, Wei Cheng Huang, Tzu Wen Huang, Hui Ying Wang, Jui Fen Lai, Te Li Chen, Tsai Ling Lauderdale

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The rate of recovery of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates has increased significantly in recent decades in Taiwan. This study investigated the molecular epidemiology of CRAB with a focus on the mechanisms of resistance and spread in isolates with blaOXA-23-like or blaOXA-24-like. All 555 CRAB isolates in our multicenter collection, which were recovered from 2002 to 2010, were tested for the presence of class A, B, and D carbapenemase genes. All isolates with blaOXA-23-like or blaOXA-24-like were subjected to pulsed-field gel electrophoresis, and 82 isolates (60 isolates with blaOXA-23-like and 22 isolates with blaOXA-24-like) were selected for multilocus sequence typing to determine the sequence type (ST) and clonal group (CG) and for detection of additional -lactamase and aminoglycoside resistance genes. The flanking regions of carbapenem and aminoglycoside resistance genes were identified by PCR mapping and sequencing. The localization of blaOXA was determined by S1 nuclease and I-CeuI assays. The numbers of CRAB isolates carrying blaOXA-23-like or blaOXA-24-like, especially those carrying blaOXA-23-like, increased significantly from 2008 onward. The blaOXA-23-like gene was carried by antibiotic resistance genomic island 1 (AbGRI1)-type structures located on plasmids and/or the chromosome in isolates of different STs (CG92 and novel CG786), whereas blaOXA-24-like was carried on plasmids in CRAB isolates of limited STs (CG92). No class A or B carbapenemase genes were identified. Multiple aminoglycoside resistance genes coexisted in CRAB. Tn6180-borne armA was found in 74 (90.2%) CRAB isolates, and 58 (70.7%) isolates had Tn6179 upstream, constituting AbGRI3. blaTEM was present in 38 (46.3%) of the CRAB isolates tested, with 35 (92.1%) isolates containing blaTEM in AbGRI2-type structures, and 61% of ampC genes had ISAba1 upstream. We conclude that the dissemination and spread of a few dominant lineages of CRAB containing various resistance island structures occurred in Taiwan.

Original languageEnglish
Article numbere01215-17
JournalAntimicrobial Agents and Chemotherapy
Volume62
Issue number3
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Acinetobacter baumannii
Carbapenems
Molecular Epidemiology
Taiwan
Aminoglycosides
Genes
Plasmids
Genomic Islands
Multilocus Sequence Typing
Pulsed Field Gel Electrophoresis
Microbial Drug Resistance
Islands
Chromosomes

Keywords

  • AbGRI
  • Acinetobacter baumannii
  • Aminoglycoside resistance
  • Carbapenem resistance
  • Transposon

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Molecular epidemiology of emerging blaOXA-23-Like- and blaOXA-24-Like-carrying acinetobacter baumannii in Taiwan. / Kuo, Shu Chen; Huang, Wei Cheng; Huang, Tzu Wen; Wang, Hui Ying; Lai, Jui Fen; Chen, Te Li; Lauderdale, Tsai Ling.

In: Antimicrobial Agents and Chemotherapy, Vol. 62, No. 3, e01215-17, 01.03.2018.

Research output: Contribution to journalArticle

Kuo, Shu Chen ; Huang, Wei Cheng ; Huang, Tzu Wen ; Wang, Hui Ying ; Lai, Jui Fen ; Chen, Te Li ; Lauderdale, Tsai Ling. / Molecular epidemiology of emerging blaOXA-23-Like- and blaOXA-24-Like-carrying acinetobacter baumannii in Taiwan. In: Antimicrobial Agents and Chemotherapy. 2018 ; Vol. 62, No. 3.
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