Molecular Epidemiology of Clinical Isolates of Methicillin-Resistant Staphylococcus aureus in Taiwan

Yhu Chering Huang, Lin Hui Su, Tsu Lan Wu, Chun Eng Liu, Tzuu Guang Young, Po Yen Chen, Po Ren Hseuh, Tzou Yien Lin

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42 Citations (Scopus)

Abstract

During July 2000 and October 2001, a total of 595 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were collected from six medical centers distributed in northern, central, and southern Taiwan. Specimen sources included blood (n = 279), pus (n = 173), sputum (n = 94), body fluids (n = 21), catheter tips (n = 20), and urine (n = 8). Pulsed-field gel electrophoresis (PFGE) with SmaI digestion was used to fingerprint these isolates. A total of 31 genotypes with 97 type-subtypes were identified. Subtypes could be identified in 7 genotypes. While there were 6 to 15 genotypes in each hospital, 433 isolates (73%) were shown to belong to a major type (genotype A, with 29 subtypes). This genotype was not only the type prevailing in all six hospitals but also the predominant clone in each hospital, accounting for 46 to 89% of all isolates in each hospital. Genotype C (16 subtypes) was the second dominant genotype, accounting for 9% of all isolates, and was distributed in five hospitals. Genotypes D (11 subtypes), E (5 subtypes), and B (6 subtypes) were distributed in five, four, and three hospitals, respectively. The other 26 types (30 type-subtypes) were minor. We conclude that the majority of MRSA clinical isolates shared a common PFGE pattern, indicating the presence of a single, epidemic MRSA clone prevailing in major hospitals in Taiwan.

Original languageEnglish
Pages (from-to)307-310
Number of pages4
JournalJournal of Clinical Microbiology
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 2004
Externally publishedYes

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Molecular Epidemiology
Methicillin-Resistant Staphylococcus aureus
Taiwan
Genotype
Pulsed Field Gel Electrophoresis
Clone Cells
Suppuration
Dermatoglyphics
Body Fluids
Sputum
Digestion
Catheters
Urine

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Molecular Epidemiology of Clinical Isolates of Methicillin-Resistant Staphylococcus aureus in Taiwan. / Huang, Yhu Chering; Su, Lin Hui; Wu, Tsu Lan; Liu, Chun Eng; Young, Tzuu Guang; Chen, Po Yen; Hseuh, Po Ren; Lin, Tzou Yien.

In: Journal of Clinical Microbiology, Vol. 42, No. 1, 01.2004, p. 307-310.

Research output: Contribution to journalArticle

Huang, Yhu Chering ; Su, Lin Hui ; Wu, Tsu Lan ; Liu, Chun Eng ; Young, Tzuu Guang ; Chen, Po Yen ; Hseuh, Po Ren ; Lin, Tzou Yien. / Molecular Epidemiology of Clinical Isolates of Methicillin-Resistant Staphylococcus aureus in Taiwan. In: Journal of Clinical Microbiology. 2004 ; Vol. 42, No. 1. pp. 307-310.
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