Molecular differentiation and diversity of forcipomyia taiwana (diptera: ceratopogonidae) based on the mitochondrial cytochrome oxidase II sequence

W. B. Yeh, H. M. Lee, W. C. Tu, L. C. Tang, P. Y. Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Forcipomyia taiwana (Shiraki), a biting midge, is one of the most annoying bloodsucking pests in Taiwan. In this study, partial DNA sequences of cytochrome c oxidase II from 113 individuals collected from 11 locations around the island were analyzed to delineate the differentiation pattern and possible dispersal processes of F. taiwana in Taiwan. The uncorrected nucleotide divergences, composed of mostly transition substitutions, were high (up to 2.7%) among the samples. Average comparable variations (≈0.7%) were found within and between populations. Phylogenetic analysis suggested that several distinct lineages exist and some can be found simultaneously in some populations. A relationship between sequence divergences among populations and their relative geographical distances was observed. Moreover, haplotype diversity was high in all populations, and low to middle levels (Fst = 0.004-0.288) of genetic differentiation were found among populations. Linearized calibration from sequence divergences and phylogenetic analysis showed that different ancestral lineages of F. taiwana possibly emerged as early as 0.6 million years ago. Taken together, genetic exchanges among these divergently ancestral lineages, likely caused by recent artificial events, have possibly led to the similarly diversified compositions of F. taiwana populations all around Taiwan nowadays.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalJournal of Medical Entomology
Volume46
Issue number2
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Fingerprint

Forcipomyia
Ceratopogonidae
Electron Transport Complex IV
Diptera
cytochrome-c oxidase
Taiwan
Population
phylogeny
Islands
Haplotypes
Calibration
haplotypes
calibration
Nucleotides
nucleotides
pests
nucleotide sequences
genetic variation

Keywords

  • Cytochrome oxidase II
  • Forcipomyia taiwana
  • Genetic diversity

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)
  • Infectious Diseases
  • Parasitology

Cite this

Molecular differentiation and diversity of forcipomyia taiwana (diptera : ceratopogonidae) based on the mitochondrial cytochrome oxidase II sequence. / Yeh, W. B.; Lee, H. M.; Tu, W. C.; Tang, L. C.; Lee, P. Y.

In: Journal of Medical Entomology, Vol. 46, No. 2, 03.2009, p. 249-256.

Research output: Contribution to journalArticle

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