Molecular cytogenetics of ovarian granulosa cell tumors by comparative genomic hybridization

Yue Shan Lin, Hock Liew Eng, Yee Jee Jan, Herng Sheng Lee, William L. Ho, Chiou Ping Liou, Wen Yu Lee, Ching Cherng Tzeng

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective. Patients with stage I granulosa cell tumors (GCTs) may occasionally develop metastasis, which is hard to predict using pathologic criteria. It is interesting to elucidate whether certain chromosomal imbalances (CIs), detected by comparative genomic hybridization (CGH), could be useful prognostic markers. Methods. CGH was used to identify CI(s) in 37 adult-type GCTs from 36 women. Nonrandom CIs were compared with clinical and pathological features to evaluate their significance as a prognostic marker. Results. Twenty-two (61%) of the 36 primary tumors had CIs. One woman's tumor showed identical CIs to another tumor that occurred in contralateral ovary 2 years later, supporting a metastatic nature. The nonrandom CIs included losses of 22q (31%), 1p33-p36 (6%), 16p13.1 (6%), and 16q (6%) and gains of 14 (25%), 12 (14%), and 7p15-p21 (6%). No tumor exhibited high-level amplification. The associations between each CI and pathological features, including the growth pattern, tumor size, and mitotic activity, were not evident. The only CI repeatedly detected in tumors with metastasis was monosomy 22, which presented in 2 of the 4 cases with metastasis but also in 2 of the 5 cases without recurrence for more than 5 years. Conclusions. Monosomy 22 was the most common CI in GCTs, which often coexisted with trisomy 14 (in 55% cases). Deletion of 22q seems to be, albeit not very specific, associated with the risk of early metastases of stage I disease. The role of loss-of-function mutation(s) of certain putative tumor suppressor gene(s) on 22q is worthy of further investigations.

Original languageEnglish
Pages (from-to)68-73
Number of pages6
JournalGynecologic Oncology
Volume97
Issue number1
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Granulosa Cell Tumor
Comparative Genomic Hybridization
Cytogenetics
Neoplasm Metastasis
Monosomy
Neoplasms
Tumor Suppressor Genes
Ovary
Recurrence
Mutation
Growth

Keywords

  • CGH
  • Chromosomal imbalance
  • Comparative genomic hybridization
  • Granulosa cell tumor
  • Ovary
  • Prognosis

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

Cite this

Lin, Y. S., Eng, H. L., Jan, Y. J., Lee, H. S., Ho, W. L., Liou, C. P., ... Tzeng, C. C. (2005). Molecular cytogenetics of ovarian granulosa cell tumors by comparative genomic hybridization. Gynecologic Oncology, 97(1), 68-73. https://doi.org/10.1016/j.ygyno.2004.12.014

Molecular cytogenetics of ovarian granulosa cell tumors by comparative genomic hybridization. / Lin, Yue Shan; Eng, Hock Liew; Jan, Yee Jee; Lee, Herng Sheng; Ho, William L.; Liou, Chiou Ping; Lee, Wen Yu; Tzeng, Ching Cherng.

In: Gynecologic Oncology, Vol. 97, No. 1, 04.2005, p. 68-73.

Research output: Contribution to journalArticle

Lin, YS, Eng, HL, Jan, YJ, Lee, HS, Ho, WL, Liou, CP, Lee, WY & Tzeng, CC 2005, 'Molecular cytogenetics of ovarian granulosa cell tumors by comparative genomic hybridization', Gynecologic Oncology, vol. 97, no. 1, pp. 68-73. https://doi.org/10.1016/j.ygyno.2004.12.014
Lin, Yue Shan ; Eng, Hock Liew ; Jan, Yee Jee ; Lee, Herng Sheng ; Ho, William L. ; Liou, Chiou Ping ; Lee, Wen Yu ; Tzeng, Ching Cherng. / Molecular cytogenetics of ovarian granulosa cell tumors by comparative genomic hybridization. In: Gynecologic Oncology. 2005 ; Vol. 97, No. 1. pp. 68-73.
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abstract = "Objective. Patients with stage I granulosa cell tumors (GCTs) may occasionally develop metastasis, which is hard to predict using pathologic criteria. It is interesting to elucidate whether certain chromosomal imbalances (CIs), detected by comparative genomic hybridization (CGH), could be useful prognostic markers. Methods. CGH was used to identify CI(s) in 37 adult-type GCTs from 36 women. Nonrandom CIs were compared with clinical and pathological features to evaluate their significance as a prognostic marker. Results. Twenty-two (61{\%}) of the 36 primary tumors had CIs. One woman's tumor showed identical CIs to another tumor that occurred in contralateral ovary 2 years later, supporting a metastatic nature. The nonrandom CIs included losses of 22q (31{\%}), 1p33-p36 (6{\%}), 16p13.1 (6{\%}), and 16q (6{\%}) and gains of 14 (25{\%}), 12 (14{\%}), and 7p15-p21 (6{\%}). No tumor exhibited high-level amplification. The associations between each CI and pathological features, including the growth pattern, tumor size, and mitotic activity, were not evident. The only CI repeatedly detected in tumors with metastasis was monosomy 22, which presented in 2 of the 4 cases with metastasis but also in 2 of the 5 cases without recurrence for more than 5 years. Conclusions. Monosomy 22 was the most common CI in GCTs, which often coexisted with trisomy 14 (in 55{\%} cases). Deletion of 22q seems to be, albeit not very specific, associated with the risk of early metastases of stage I disease. The role of loss-of-function mutation(s) of certain putative tumor suppressor gene(s) on 22q is worthy of further investigations.",
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T1 - Molecular cytogenetics of ovarian granulosa cell tumors by comparative genomic hybridization

AU - Lin, Yue Shan

AU - Eng, Hock Liew

AU - Jan, Yee Jee

AU - Lee, Herng Sheng

AU - Ho, William L.

AU - Liou, Chiou Ping

AU - Lee, Wen Yu

AU - Tzeng, Ching Cherng

PY - 2005/4

Y1 - 2005/4

N2 - Objective. Patients with stage I granulosa cell tumors (GCTs) may occasionally develop metastasis, which is hard to predict using pathologic criteria. It is interesting to elucidate whether certain chromosomal imbalances (CIs), detected by comparative genomic hybridization (CGH), could be useful prognostic markers. Methods. CGH was used to identify CI(s) in 37 adult-type GCTs from 36 women. Nonrandom CIs were compared with clinical and pathological features to evaluate their significance as a prognostic marker. Results. Twenty-two (61%) of the 36 primary tumors had CIs. One woman's tumor showed identical CIs to another tumor that occurred in contralateral ovary 2 years later, supporting a metastatic nature. The nonrandom CIs included losses of 22q (31%), 1p33-p36 (6%), 16p13.1 (6%), and 16q (6%) and gains of 14 (25%), 12 (14%), and 7p15-p21 (6%). No tumor exhibited high-level amplification. The associations between each CI and pathological features, including the growth pattern, tumor size, and mitotic activity, were not evident. The only CI repeatedly detected in tumors with metastasis was monosomy 22, which presented in 2 of the 4 cases with metastasis but also in 2 of the 5 cases without recurrence for more than 5 years. Conclusions. Monosomy 22 was the most common CI in GCTs, which often coexisted with trisomy 14 (in 55% cases). Deletion of 22q seems to be, albeit not very specific, associated with the risk of early metastases of stage I disease. The role of loss-of-function mutation(s) of certain putative tumor suppressor gene(s) on 22q is worthy of further investigations.

AB - Objective. Patients with stage I granulosa cell tumors (GCTs) may occasionally develop metastasis, which is hard to predict using pathologic criteria. It is interesting to elucidate whether certain chromosomal imbalances (CIs), detected by comparative genomic hybridization (CGH), could be useful prognostic markers. Methods. CGH was used to identify CI(s) in 37 adult-type GCTs from 36 women. Nonrandom CIs were compared with clinical and pathological features to evaluate their significance as a prognostic marker. Results. Twenty-two (61%) of the 36 primary tumors had CIs. One woman's tumor showed identical CIs to another tumor that occurred in contralateral ovary 2 years later, supporting a metastatic nature. The nonrandom CIs included losses of 22q (31%), 1p33-p36 (6%), 16p13.1 (6%), and 16q (6%) and gains of 14 (25%), 12 (14%), and 7p15-p21 (6%). No tumor exhibited high-level amplification. The associations between each CI and pathological features, including the growth pattern, tumor size, and mitotic activity, were not evident. The only CI repeatedly detected in tumors with metastasis was monosomy 22, which presented in 2 of the 4 cases with metastasis but also in 2 of the 5 cases without recurrence for more than 5 years. Conclusions. Monosomy 22 was the most common CI in GCTs, which often coexisted with trisomy 14 (in 55% cases). Deletion of 22q seems to be, albeit not very specific, associated with the risk of early metastases of stage I disease. The role of loss-of-function mutation(s) of certain putative tumor suppressor gene(s) on 22q is worthy of further investigations.

KW - CGH

KW - Chromosomal imbalance

KW - Comparative genomic hybridization

KW - Granulosa cell tumor

KW - Ovary

KW - Prognosis

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