Molecular cloning and expression of a sweet potato cysteine protease SPCP1 from senescent leaves

Hsien Jung Chen, Guan Jhong Huang, Wei Shan Chen, Cheng Ting Su, Wen Chi Hou, Yaw Huei Lin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In this report a full-length cDNA, SPCP1, was isolated from senescent leaves of sweet potato (Ipomoea batatas (L.) Lam). SPCP1 contained 1020 nucleotides (339 amino acids) in the open reading frame, and exhibited high amino acid sequence homologies (ca. 58% to 74%) with papain-like cysteine proteases of Alnus glutinosa, Arabidopsis thaliana, Astragalus sinicus, Brassica napus, Daucus carota, Gossypium hirsutum, Hordeum vulgare, Iris hollandica, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Ricinus communis, Trifolium repens. Semi-quantitative RT-PCR and Western blot hybridization showed that SPCP1 gene expression was enhanced significantly in natural senescent leaves and in dark-, ethephon-, and ABAinduced senescent leaves, whereas, was almost not detected in mature green leaves, stems, and roots. Initiation of chlorophyll degradation is earlier than the SPCP1 gene expression during leaf senescence. SPCP1 expression was also induced in sweet potato suspension cells treated with 1 mM ethephon. Evan blue staining showed that suspension cells were not significantly affected by ethephon treatment up to 2 mM, however, most of the cells died when treated with 10 mM ethephon. In conclusion, sweet potato SPCP1 is likely a functional, senescence-associated gene and its expression levels were significantly enhanced at mRNA and protein levels in natural and induced senescent leaves and suspension cells. The physiological role and function of SPCP1 were likely not in association with initiation of chlorophyll degradation and cell death during senescence.

Original languageEnglish
Pages (from-to)159-170
Number of pages12
JournalBotanical Studies
Volume50
Issue number2
Publication statusPublished - Apr 2009

Fingerprint

cysteine proteinases
sweet potatoes
molecular cloning
ethephon
cell suspension culture
leaves
Iris hollandica
Astragalus sinicus
chlorophyll
gene expression
Alnus glutinosa
degradation
Ipomoea batatas
papain
Ricinus communis
Medicago truncatula
Daucus carota
Trifolium repens
sequence homology
Gossypium hirsutum

Keywords

  • Cysteine protease
  • Ethephon
  • Leaf senescence
  • SPCP1
  • Sweet potato

ASJC Scopus subject areas

  • Plant Science

Cite this

Chen, H. J., Huang, G. J., Chen, W. S., Su, C. T., Hou, W. C., & Lin, Y. H. (2009). Molecular cloning and expression of a sweet potato cysteine protease SPCP1 from senescent leaves. Botanical Studies, 50(2), 159-170.

Molecular cloning and expression of a sweet potato cysteine protease SPCP1 from senescent leaves. / Chen, Hsien Jung; Huang, Guan Jhong; Chen, Wei Shan; Su, Cheng Ting; Hou, Wen Chi; Lin, Yaw Huei.

In: Botanical Studies, Vol. 50, No. 2, 04.2009, p. 159-170.

Research output: Contribution to journalArticle

Chen, HJ, Huang, GJ, Chen, WS, Su, CT, Hou, WC & Lin, YH 2009, 'Molecular cloning and expression of a sweet potato cysteine protease SPCP1 from senescent leaves', Botanical Studies, vol. 50, no. 2, pp. 159-170.
Chen, Hsien Jung ; Huang, Guan Jhong ; Chen, Wei Shan ; Su, Cheng Ting ; Hou, Wen Chi ; Lin, Yaw Huei. / Molecular cloning and expression of a sweet potato cysteine protease SPCP1 from senescent leaves. In: Botanical Studies. 2009 ; Vol. 50, No. 2. pp. 159-170.
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