Molecular-clinical correlation in pediatric medulloblastoma: A cohort series study of 52 cases in Taiwan

Kuo Sheng Wu, Donald Ming Tak Ho, Shiann Tarng Jou, Alice L. Yu, Huy Minh Tran, Muh Lii Liang, Hsin Hung Chen, Yi Yen Lee, Yi Wei Chen, Shih Chieh Lin, Feng Chi Chang, Min Lan Tsai, Yen Lin Liu, Hsin Lun Lee, Kevin Li Chun Hsieh, Wen Chang Huang, Shian Ying Sung, Che Chang Chang, Chun Austin Changou, Kung Hao LiangTsung Han Hsieh, Yun Ru Liu, Meng En Chao, Wan Chen, Shing Shung Chu, Er Chieh Cho, Tai Tong Wong

Research output: Contribution to journalArticlepeer-review

Abstract

In 2016, a project was initiated in Taiwan to adopt molecular diagnosis of childhood medulloblastoma (MB). In this study, we aimed to identify a molecular-clinical correlation and somatic mutation for exploring risk-adapted treatment, drug targets, and potential genetic predisposition. In total, 52 frozen tumor tissues of childhood MBs were collected. RNA sequencing (RNA-Seq) and DNA methylation array data were generated. Molecular subgrouping and clinical correlation analysis were performed. An adjusted Heidelberg risk stratification scheme was defined for updated clinical risk stratification. We selected 51 genes for somatic variant calling using RNA-Seq data. Relevant clinical findings were defined. Potential drug targets and genetic predispositions were explored. Four core molecular subgroups (WNT, SHH, Group 3, and Group 4) were identified. Genetic backgrounds of metastasis at diagnosis and extent of tumor resection were observed. The adjusted Heidelberg scheme showed its applicability. Potential drug targets were detected in the pathways of DNA damage response. Among the 10 patients with SHH MBs analyzed using whole exome sequencing studies, five patients exhibited potential genetic predispositions and four patients had relevant germline mutations. The findings of this study provide valuable information for updated risk adapted treatment and personalized care of childhood MBs in our cohort series and in Taiwan.

Original languageEnglish
Article number653
JournalCancers
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 2020

Keywords

  • Clinical correlation
  • DNA damage response
  • Genetic predisposition
  • Medulloblastoma
  • Molecular
  • Risk stratification
  • RNA-Seq
  • Somatic mutations

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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