Molecular analysis of mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexa

P. M. Chen, T. J. Chiou, I. T. Yu, F. S. Fan, C. J. Chu, S. C. Kao, W. S. Wang, J. H. Liu, Wen-Ming Hsu, M. H. Yang, T. C. Chao, C. J. Tai, L. T. Hsiao, J. T. Lin, C. C. Yen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Lymphomas of mucosa-associated lymphoid tissue (MALT) are a distinct subgroup of extranodal B-cell non-Hodgkin's lymphomas. Most studies have failed to demonstrate the clonal rearrangement of BCL-1, BCL-2 or c-MYC genes for MALT lymphomas. Further, alteration of the p53 gene is rarely demonstrated in low-grade MALT lymphomas, but can be detected in high-grade disease. Lymphomas of the ocular adnexa represent approximately eight percent of all extranodal lymphomas, most of which are MALT lymphomas, but few studies had explored the alterations of BCL-1, BCL-2, c-MYC and p53 genes specifically for ocular MALT lymphomas. We investigated the changes to BCL-1, BCL-2, c-MYC and p53 genes in these lymphomas for Taiwanese patients. Clonal rearrangement for immunoglobulin heavy-chain (IgH), BCL-1, BCL-2, c-MYC and p53 genes was examined for 16 cases of ocular MALT lymphoma. Restriction-length polymorphism and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) of the DNA, corresponding to exons 5 through 9, followed by DNA sequencing, were utilized to analyze the possible mutations of the p53 gene for these tumors. Thirteen of the cases revealed rearranged IgH genes using Southern blotting or PCR. No rearrangement of BCL-1, BCL-2, c-MYC or p53 genes was discovered, with point mutation of the p53 gene in one case. As for other types of MALT lymphomas, BCL-1, BCL-2 and c-MYC genes are not implicated in the pathogenesis of the ocular sub-group. Although alteration of the p53 gene is rare for low-grade ocular MALT lymphoma, its role in disease progression merits further research.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalLeukemia and Lymphoma
Volume42
Issue number1-2
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Marginal Zone B-Cell Lymphoma
p53 Genes
Lymphoma
Non-Hodgkin's Lymphoma
Immunoglobulin Heavy Chain Genes
Nucleic Acid Conformation
Polymerase Chain Reaction
Immunoglobulin Heavy Chains
B-Cell Lymphoma
Southern Blotting
DNA Sequence Analysis
Point Mutation
Genes
Disease Progression
Exons
Mutation

Keywords

  • Gene rearrangement
  • Lymphoma
  • Mucosa-associated lymphoid tissue
  • Orbital neoplasm
  • p53
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Chen, P. M., Chiou, T. J., Yu, I. T., Fan, F. S., Chu, C. J., Kao, S. C., ... Yen, C. C. (2001). Molecular analysis of mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexa. Leukemia and Lymphoma, 42(1-2), 207-214.

Molecular analysis of mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexa. / Chen, P. M.; Chiou, T. J.; Yu, I. T.; Fan, F. S.; Chu, C. J.; Kao, S. C.; Wang, W. S.; Liu, J. H.; Hsu, Wen-Ming; Yang, M. H.; Chao, T. C.; Tai, C. J.; Hsiao, L. T.; Lin, J. T.; Yen, C. C.

In: Leukemia and Lymphoma, Vol. 42, No. 1-2, 2001, p. 207-214.

Research output: Contribution to journalArticle

Chen, PM, Chiou, TJ, Yu, IT, Fan, FS, Chu, CJ, Kao, SC, Wang, WS, Liu, JH, Hsu, W-M, Yang, MH, Chao, TC, Tai, CJ, Hsiao, LT, Lin, JT & Yen, CC 2001, 'Molecular analysis of mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexa', Leukemia and Lymphoma, vol. 42, no. 1-2, pp. 207-214.
Chen PM, Chiou TJ, Yu IT, Fan FS, Chu CJ, Kao SC et al. Molecular analysis of mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexa. Leukemia and Lymphoma. 2001;42(1-2):207-214.
Chen, P. M. ; Chiou, T. J. ; Yu, I. T. ; Fan, F. S. ; Chu, C. J. ; Kao, S. C. ; Wang, W. S. ; Liu, J. H. ; Hsu, Wen-Ming ; Yang, M. H. ; Chao, T. C. ; Tai, C. J. ; Hsiao, L. T. ; Lin, J. T. ; Yen, C. C. / Molecular analysis of mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexa. In: Leukemia and Lymphoma. 2001 ; Vol. 42, No. 1-2. pp. 207-214.
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