Modulatory Effects of Astragalus Polysaccharides on T-Cell Polarization in Mice with Polymicrobial Sepsis

Yu-Chen Hou, Jin Ming Wu, Ming Yang Wang, Ming Hsun Wu, Kuen Yuan Chen, Sung Ling Yeh, Ming Tsan Lin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background. This study evaluated the impact of different doses of Astragalus polysaccharides (APS) on the functional status and phenotype of T cells during polymicrobial sepsis. Methods. On day 1 after cecal ligation and puncture, mice were treated with either saline, 100 (A100), 200 (A200), or 400 mg APS/kg body weight (BW) (A400) by an intraperitoneal injection daily for 4 days. All mice were sacrificed 5 days after the operation. Results. APS treatment reversed the sepsis-induced decrement in the T helper (Th) cell population, and the percentage of activated Th cells also increased in the spleen and Peyer's patches. APS administration downregulated the percentages of circulating Th2 cells and regulatory T cells (Treg), and the percentage of Th17 cells in blood was upregulated in the A400 group. Weight loss and kidney injury were attenuated in the A100 and A200 groups but not in the A400 group at the end of the study. Conclusions. Treatments with 100 and 200 mg APS/kg BW reduced Treg populations and elicited a more-balanced Th1/Th2 response that consequently attenuated immunosuppression in polymicrobial sepsis. High-dose APS administration led to excessive responses of Th17 cells which may have adverse effects in sepsis-induced organ injury.

Original languageEnglish
Article number826319
JournalMediators of Inflammation
Volume2015
DOIs
Publication statusPublished - 2015

Fingerprint

Polysaccharides
Sepsis
T-Lymphocytes
Th17 Cells
Helper-Inducer T-Lymphocytes
Body Weight
Peyer's Patches
Th2 Cells
Wounds and Injuries
Regulatory T-Lymphocytes
Intraperitoneal Injections
Punctures
Immunosuppression
Population
Ligation
Weight Loss
Down-Regulation
Spleen
Phenotype
Kidney

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Modulatory Effects of Astragalus Polysaccharides on T-Cell Polarization in Mice with Polymicrobial Sepsis. / Hou, Yu-Chen; Wu, Jin Ming; Wang, Ming Yang; Wu, Ming Hsun; Chen, Kuen Yuan; Yeh, Sung Ling; Lin, Ming Tsan.

In: Mediators of Inflammation, Vol. 2015, 826319, 2015.

Research output: Contribution to journalArticle

Hou, Yu-Chen ; Wu, Jin Ming ; Wang, Ming Yang ; Wu, Ming Hsun ; Chen, Kuen Yuan ; Yeh, Sung Ling ; Lin, Ming Tsan. / Modulatory Effects of Astragalus Polysaccharides on T-Cell Polarization in Mice with Polymicrobial Sepsis. In: Mediators of Inflammation. 2015 ; Vol. 2015.
@article{f8f88413711848df95c707ccc1b7fa17,
title = "Modulatory Effects of Astragalus Polysaccharides on T-Cell Polarization in Mice with Polymicrobial Sepsis",
abstract = "Background. This study evaluated the impact of different doses of Astragalus polysaccharides (APS) on the functional status and phenotype of T cells during polymicrobial sepsis. Methods. On day 1 after cecal ligation and puncture, mice were treated with either saline, 100 (A100), 200 (A200), or 400 mg APS/kg body weight (BW) (A400) by an intraperitoneal injection daily for 4 days. All mice were sacrificed 5 days after the operation. Results. APS treatment reversed the sepsis-induced decrement in the T helper (Th) cell population, and the percentage of activated Th cells also increased in the spleen and Peyer's patches. APS administration downregulated the percentages of circulating Th2 cells and regulatory T cells (Treg), and the percentage of Th17 cells in blood was upregulated in the A400 group. Weight loss and kidney injury were attenuated in the A100 and A200 groups but not in the A400 group at the end of the study. Conclusions. Treatments with 100 and 200 mg APS/kg BW reduced Treg populations and elicited a more-balanced Th1/Th2 response that consequently attenuated immunosuppression in polymicrobial sepsis. High-dose APS administration led to excessive responses of Th17 cells which may have adverse effects in sepsis-induced organ injury.",
author = "Yu-Chen Hou and Wu, {Jin Ming} and Wang, {Ming Yang} and Wu, {Ming Hsun} and Chen, {Kuen Yuan} and Yeh, {Sung Ling} and Lin, {Ming Tsan}",
year = "2015",
doi = "10.1155/2015/826319",
language = "English",
volume = "2015",
journal = "Mediators of Inflammation",
issn = "0962-9351",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Modulatory Effects of Astragalus Polysaccharides on T-Cell Polarization in Mice with Polymicrobial Sepsis

AU - Hou, Yu-Chen

AU - Wu, Jin Ming

AU - Wang, Ming Yang

AU - Wu, Ming Hsun

AU - Chen, Kuen Yuan

AU - Yeh, Sung Ling

AU - Lin, Ming Tsan

PY - 2015

Y1 - 2015

N2 - Background. This study evaluated the impact of different doses of Astragalus polysaccharides (APS) on the functional status and phenotype of T cells during polymicrobial sepsis. Methods. On day 1 after cecal ligation and puncture, mice were treated with either saline, 100 (A100), 200 (A200), or 400 mg APS/kg body weight (BW) (A400) by an intraperitoneal injection daily for 4 days. All mice were sacrificed 5 days after the operation. Results. APS treatment reversed the sepsis-induced decrement in the T helper (Th) cell population, and the percentage of activated Th cells also increased in the spleen and Peyer's patches. APS administration downregulated the percentages of circulating Th2 cells and regulatory T cells (Treg), and the percentage of Th17 cells in blood was upregulated in the A400 group. Weight loss and kidney injury were attenuated in the A100 and A200 groups but not in the A400 group at the end of the study. Conclusions. Treatments with 100 and 200 mg APS/kg BW reduced Treg populations and elicited a more-balanced Th1/Th2 response that consequently attenuated immunosuppression in polymicrobial sepsis. High-dose APS administration led to excessive responses of Th17 cells which may have adverse effects in sepsis-induced organ injury.

AB - Background. This study evaluated the impact of different doses of Astragalus polysaccharides (APS) on the functional status and phenotype of T cells during polymicrobial sepsis. Methods. On day 1 after cecal ligation and puncture, mice were treated with either saline, 100 (A100), 200 (A200), or 400 mg APS/kg body weight (BW) (A400) by an intraperitoneal injection daily for 4 days. All mice were sacrificed 5 days after the operation. Results. APS treatment reversed the sepsis-induced decrement in the T helper (Th) cell population, and the percentage of activated Th cells also increased in the spleen and Peyer's patches. APS administration downregulated the percentages of circulating Th2 cells and regulatory T cells (Treg), and the percentage of Th17 cells in blood was upregulated in the A400 group. Weight loss and kidney injury were attenuated in the A100 and A200 groups but not in the A400 group at the end of the study. Conclusions. Treatments with 100 and 200 mg APS/kg BW reduced Treg populations and elicited a more-balanced Th1/Th2 response that consequently attenuated immunosuppression in polymicrobial sepsis. High-dose APS administration led to excessive responses of Th17 cells which may have adverse effects in sepsis-induced organ injury.

UR - http://www.scopus.com/inward/record.url?scp=84949267434&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949267434&partnerID=8YFLogxK

U2 - 10.1155/2015/826319

DO - 10.1155/2015/826319

M3 - Article

VL - 2015

JO - Mediators of Inflammation

JF - Mediators of Inflammation

SN - 0962-9351

M1 - 826319

ER -