Modulation of the proliferation and matrix synthesis of chondrocytes by dynamic compression on genipin-crosslinked chitosan/collagen scaffolds

Peng YuanWang And, Wei Bor Tsai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Dynamic compression is an important physical stimulus for the physiology of chondrocyte and articular cartilage tissue engineering. In this study, modulation of chondrocyte behaviors in chitosan/collagen scaffolds with different mechanical properties under free-swelling or dynamic compression conditions was investigated. Rabbit chondrocytes were seeded in chitosan/collagen scaffolds crosslinked by genipin (GP) with different concentrations, and then cultured for 3 days prior to cyclic compression of 40% strain, 0.1 Hz, and 30min/day for 2 weeks. The results showed that the cell proliferation was increased with increasing genipin concentrations and dynamic compression. On the other hand, although total glycosaminoglycans GAGs deposition was enhanced by dynamic compression under certain conditions, e.g. the GP0.5 chitosan/collagen scaffolds for 1 week of compression culture, normalized GAGs deposition per cell was decreased by dynamic compression. Our results suggest that while several studies suggest that dynamic compression benefits articular cartilage tissue engineering, many factors including scaffold types and compression conditions determine the outcome of dynamic compression culture.

Original languageEnglish
Pages (from-to)507-519
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Volume24
Issue number5
DOIs
Publication statusPublished - Apr 2013
Externally publishedYes

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Keywords

  • Chondrocytes
  • Dynamic compression
  • Elastic scaffold; glycosaminoglycan
  • Mechanical property

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

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