Modulation of Monocyte-Derived Dendritic Cell Differentiation is Associated With Ischemic Acute Renal Failure

Chih J. Wu, J. R. Sheu, Han Hsiang Chen, Hui F. Liao, Yuh Cheng Yang, Stone Yang, Yu J. Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Dendritic cells (DCs) play a central role in both stimulating and suppressing immune responses and are impacted by surgical injury, exercise, and other physiological stressors. This study aims to determine whether renal ischemia/reperfusion (I/R) injury alters the differentiation, maturation, and activation of DCs from peripheral blood monocytes (PBMo). Materials and methods: Sprague-Dawley (SD) rats were subjected to I/R injury or sham-operated. Creatinine clearance (CCr) was monitored daily during the 14 days of reperfusion that followed the ischemic insult. At 2 and 14 days of reperfusion, the following properties of PBMo derived-DCs were assessed: the amount of generated DCs, surface markers [CD11c, CD80, CD86, and MHC-II (IA)], and functional status including magnitude of mixed lymphocyte reaction (MLR), production of IL-12 p70 by DCs, and production of IFN-γ and IL-4 by DC-stimulated T cells. Results: CCr was greatly reduced in the injured rats 0 to 4 days after ischemia. Two days after I/R injury to kidney, the numbers of DCs differentiated from PBMo, IL-12 production by DCs, expression of MHC-II (IA), and IFN-γ production by DC-stimulated T cells were significantly increased in the I/R injured group (compared to the sham-operated group). After 14 days of reperfusion, there was no between-group differences in the numbers of DCs derived from PBMo, MLR, expression of CD80, CD86, and MHC-II (IA), and production of IL-12, IFN-γ, and IL-4. Conclusions: The increases seen at 2 days of reperfusion may reflect a preparatory step in the renal I/R injury pathway. The relationship between up-regulation of DC differentiation and ischemic acute renal failure (ARF) remains to be elucidated.

Original languageEnglish
Pages (from-to)104-111
Number of pages8
JournalJournal of Surgical Research
Volume132
Issue number1
DOIs
Publication statusPublished - May 2006

Fingerprint

Acute Kidney Injury
Dendritic Cells
Monocytes
Cell Differentiation
Reperfusion
Reperfusion Injury
Interleukin-12
Mixed Lymphocyte Culture Test
Kidney
Interleukin-4
Creatinine
Ischemia
T-Lymphocytes
Intraoperative Complications
Sprague Dawley Rats
Up-Regulation

Keywords

  • dendritic cells
  • differentiation
  • ischemia-reperfusion injury

ASJC Scopus subject areas

  • Surgery

Cite this

Modulation of Monocyte-Derived Dendritic Cell Differentiation is Associated With Ischemic Acute Renal Failure. / Wu, Chih J.; Sheu, J. R.; Chen, Han Hsiang; Liao, Hui F.; Yang, Yuh Cheng; Yang, Stone; Chen, Yu J.

In: Journal of Surgical Research, Vol. 132, No. 1, 05.2006, p. 104-111.

Research output: Contribution to journalArticle

Wu, Chih J. ; Sheu, J. R. ; Chen, Han Hsiang ; Liao, Hui F. ; Yang, Yuh Cheng ; Yang, Stone ; Chen, Yu J. / Modulation of Monocyte-Derived Dendritic Cell Differentiation is Associated With Ischemic Acute Renal Failure. In: Journal of Surgical Research. 2006 ; Vol. 132, No. 1. pp. 104-111.
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AU - Yang, Yuh Cheng

AU - Yang, Stone

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AB - Background: Dendritic cells (DCs) play a central role in both stimulating and suppressing immune responses and are impacted by surgical injury, exercise, and other physiological stressors. This study aims to determine whether renal ischemia/reperfusion (I/R) injury alters the differentiation, maturation, and activation of DCs from peripheral blood monocytes (PBMo). Materials and methods: Sprague-Dawley (SD) rats were subjected to I/R injury or sham-operated. Creatinine clearance (CCr) was monitored daily during the 14 days of reperfusion that followed the ischemic insult. At 2 and 14 days of reperfusion, the following properties of PBMo derived-DCs were assessed: the amount of generated DCs, surface markers [CD11c, CD80, CD86, and MHC-II (IA)], and functional status including magnitude of mixed lymphocyte reaction (MLR), production of IL-12 p70 by DCs, and production of IFN-γ and IL-4 by DC-stimulated T cells. Results: CCr was greatly reduced in the injured rats 0 to 4 days after ischemia. Two days after I/R injury to kidney, the numbers of DCs differentiated from PBMo, IL-12 production by DCs, expression of MHC-II (IA), and IFN-γ production by DC-stimulated T cells were significantly increased in the I/R injured group (compared to the sham-operated group). After 14 days of reperfusion, there was no between-group differences in the numbers of DCs derived from PBMo, MLR, expression of CD80, CD86, and MHC-II (IA), and production of IL-12, IFN-γ, and IL-4. Conclusions: The increases seen at 2 days of reperfusion may reflect a preparatory step in the renal I/R injury pathway. The relationship between up-regulation of DC differentiation and ischemic acute renal failure (ARF) remains to be elucidated.

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