Modulation of hepatic phase II phenol sulfotransferase and antioxidant status by phenolic acids in rats

Chi Tai Yeh, Gow Chin Yen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Phenolic acids have significant biological and pharmacological properties and some have demonstrated remarkable ability to alter sulfate conjugation. However, the modulatory effects of phenolic acids on phenol sulfotransferases (PSTs) in vivo have not been described. The present investigation evaluates the role of phenolic acid on the expression of PSTs in male Sprague-Dawley rat liver. According to the results, gentisic acid, gallic acid and p-coumaric acid in a dosage of 100 mg/kg of body weight for 14 consecutive days significantly increased P-form PST (PST-P) activity as compared with that of the control rats (P<.05), whereas the activity of M-form PST (PST-M) in rats that received gallic acid and p-coumaric acid were also significantly (P<.05) higher than in the control rats. Reverse transcriptase-polymerase chain reaction results indicated that the changes in both PST-P and PST-M mRNA levels by phenolic acids were similar to those noted in the enzyme activity levels. The plasma obtained from phenolic acid-administered rats had significantly (P<.05) increased oxygen radical absorbance capacity (ORAC ROO {radical dot}) values than that from control rats. In a bioavailability study, following oral administration of gallic acid and p-coumaric acid, the phenolic acids were detected in the plasma, and the C max values after 2.0-h administration were 665±23 and 550±33 nmol/L, respectively. There was a significant correlation between the activity of both forms of PSTs and the antioxidant capacity of ORAC ROO {radical dot} value by phenolic acids (r=.74, P<.05 and r=.77, P<.05). These data suggest that phenolic acids might alter sulfate conjugation and antioxidant capacity in living systems.

Original languageEnglish
Pages (from-to)561-569
Number of pages9
JournalJournal of Nutritional Biochemistry
Volume17
Issue number8
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

Fingerprint

Arylsulfotransferase
Rats
Antioxidants
Modulation
Liver
Rat control
Gallic Acid
Sulfates
Plasmas
phenolic acid
Polymerase chain reaction
RNA-Directed DNA Polymerase
Enzyme activity
Reverse Transcriptase Polymerase Chain Reaction
Biological Availability
Oral Administration
Sprague Dawley Rats
Reactive Oxygen Species
Body Weight
Pharmacology

Keywords

  • Antioxidant capacity
  • Bioavailability
  • Phenol sulfotransferase
  • Phenolic acids
  • Sprague-Dawley rat

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Modulation of hepatic phase II phenol sulfotransferase and antioxidant status by phenolic acids in rats. / Yeh, Chi Tai; Yen, Gow Chin.

In: Journal of Nutritional Biochemistry, Vol. 17, No. 8, 08.2006, p. 561-569.

Research output: Contribution to journalArticle

@article{e582f704639c43ab8634c7250f67c8a7,
title = "Modulation of hepatic phase II phenol sulfotransferase and antioxidant status by phenolic acids in rats",
abstract = "Phenolic acids have significant biological and pharmacological properties and some have demonstrated remarkable ability to alter sulfate conjugation. However, the modulatory effects of phenolic acids on phenol sulfotransferases (PSTs) in vivo have not been described. The present investigation evaluates the role of phenolic acid on the expression of PSTs in male Sprague-Dawley rat liver. According to the results, gentisic acid, gallic acid and p-coumaric acid in a dosage of 100 mg/kg of body weight for 14 consecutive days significantly increased P-form PST (PST-P) activity as compared with that of the control rats (P<.05), whereas the activity of M-form PST (PST-M) in rats that received gallic acid and p-coumaric acid were also significantly (P<.05) higher than in the control rats. Reverse transcriptase-polymerase chain reaction results indicated that the changes in both PST-P and PST-M mRNA levels by phenolic acids were similar to those noted in the enzyme activity levels. The plasma obtained from phenolic acid-administered rats had significantly (P<.05) increased oxygen radical absorbance capacity (ORAC ROO {radical dot}) values than that from control rats. In a bioavailability study, following oral administration of gallic acid and p-coumaric acid, the phenolic acids were detected in the plasma, and the C max values after 2.0-h administration were 665±23 and 550±33 nmol/L, respectively. There was a significant correlation between the activity of both forms of PSTs and the antioxidant capacity of ORAC ROO {radical dot} value by phenolic acids (r=.74, P<.05 and r=.77, P<.05). These data suggest that phenolic acids might alter sulfate conjugation and antioxidant capacity in living systems.",
keywords = "Antioxidant capacity, Bioavailability, Phenol sulfotransferase, Phenolic acids, Sprague-Dawley rat",
author = "Yeh, {Chi Tai} and Yen, {Gow Chin}",
year = "2006",
month = "8",
doi = "10.1016/j.jnutbio.2005.10.008",
language = "English",
volume = "17",
pages = "561--569",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - Modulation of hepatic phase II phenol sulfotransferase and antioxidant status by phenolic acids in rats

AU - Yeh, Chi Tai

AU - Yen, Gow Chin

PY - 2006/8

Y1 - 2006/8

N2 - Phenolic acids have significant biological and pharmacological properties and some have demonstrated remarkable ability to alter sulfate conjugation. However, the modulatory effects of phenolic acids on phenol sulfotransferases (PSTs) in vivo have not been described. The present investigation evaluates the role of phenolic acid on the expression of PSTs in male Sprague-Dawley rat liver. According to the results, gentisic acid, gallic acid and p-coumaric acid in a dosage of 100 mg/kg of body weight for 14 consecutive days significantly increased P-form PST (PST-P) activity as compared with that of the control rats (P<.05), whereas the activity of M-form PST (PST-M) in rats that received gallic acid and p-coumaric acid were also significantly (P<.05) higher than in the control rats. Reverse transcriptase-polymerase chain reaction results indicated that the changes in both PST-P and PST-M mRNA levels by phenolic acids were similar to those noted in the enzyme activity levels. The plasma obtained from phenolic acid-administered rats had significantly (P<.05) increased oxygen radical absorbance capacity (ORAC ROO {radical dot}) values than that from control rats. In a bioavailability study, following oral administration of gallic acid and p-coumaric acid, the phenolic acids were detected in the plasma, and the C max values after 2.0-h administration were 665±23 and 550±33 nmol/L, respectively. There was a significant correlation between the activity of both forms of PSTs and the antioxidant capacity of ORAC ROO {radical dot} value by phenolic acids (r=.74, P<.05 and r=.77, P<.05). These data suggest that phenolic acids might alter sulfate conjugation and antioxidant capacity in living systems.

AB - Phenolic acids have significant biological and pharmacological properties and some have demonstrated remarkable ability to alter sulfate conjugation. However, the modulatory effects of phenolic acids on phenol sulfotransferases (PSTs) in vivo have not been described. The present investigation evaluates the role of phenolic acid on the expression of PSTs in male Sprague-Dawley rat liver. According to the results, gentisic acid, gallic acid and p-coumaric acid in a dosage of 100 mg/kg of body weight for 14 consecutive days significantly increased P-form PST (PST-P) activity as compared with that of the control rats (P<.05), whereas the activity of M-form PST (PST-M) in rats that received gallic acid and p-coumaric acid were also significantly (P<.05) higher than in the control rats. Reverse transcriptase-polymerase chain reaction results indicated that the changes in both PST-P and PST-M mRNA levels by phenolic acids were similar to those noted in the enzyme activity levels. The plasma obtained from phenolic acid-administered rats had significantly (P<.05) increased oxygen radical absorbance capacity (ORAC ROO {radical dot}) values than that from control rats. In a bioavailability study, following oral administration of gallic acid and p-coumaric acid, the phenolic acids were detected in the plasma, and the C max values after 2.0-h administration were 665±23 and 550±33 nmol/L, respectively. There was a significant correlation between the activity of both forms of PSTs and the antioxidant capacity of ORAC ROO {radical dot} value by phenolic acids (r=.74, P<.05 and r=.77, P<.05). These data suggest that phenolic acids might alter sulfate conjugation and antioxidant capacity in living systems.

KW - Antioxidant capacity

KW - Bioavailability

KW - Phenol sulfotransferase

KW - Phenolic acids

KW - Sprague-Dawley rat

UR - http://www.scopus.com/inward/record.url?scp=33746031011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746031011&partnerID=8YFLogxK

U2 - 10.1016/j.jnutbio.2005.10.008

DO - 10.1016/j.jnutbio.2005.10.008

M3 - Article

C2 - 16443358

AN - SCOPUS:33746031011

VL - 17

SP - 561

EP - 569

JO - Journal of Nutritional Biochemistry

JF - Journal of Nutritional Biochemistry

SN - 0955-2863

IS - 8

ER -