Modulation of cytokine secretion by garlic oil derivatives is associated with suppressed nitric oxide production in stimulated macrophages

Hsiao Pei Chang, Shih Yi Huang, Yue Hwa Chen

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41 Citations (Scopus)

Abstract

We previously described that garlic oil derivatives differentially suppress the production of nitric oxide (NO) and prostaglandin E2 (PGE 2) in activated macrophages. In the present study, we investigated the effects of the garlic derivatives, diallyl sulfide (DAS), diallyl disulfide (DADS), and allyl methyl sulfide (AMS), on cytokine production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and the association between modulation of cytokines and inhibition of NO production was also assessed. The results indicated that these garlic compounds had different effects on the secretion of activated cytokines, including proinflammatory tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6, as well as the antiinflammatory, IL-10. DAS inhibited the production of all stimulated cytokines in a concentration-dependent manner, and the inhibition was closely associated with the suppression of NO and PGE2 production. DADS repressed the production of stimulated TNF-α and IL-10 and increased the production of activated IL-1β and, to a lesser extent, IL-6; but only the decreased IL-10 production was associated with DADS-induced NO inhibition. Yet, the DAS- and DADS-suppressed NO production was independent of TNF-α. AMS, on the other hand, slightly suppressed the stimulated TNF-α but enhanced IL-10 production, and such modulation was closely associated with the decrease in NO production.

Original languageEnglish
Pages (from-to)2530-2534
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number7
DOIs
Publication statusPublished - Apr 6 2005

Fingerprint

Macrophages
garlic
nitric oxide
Nitric Oxide
macrophages
cytokines
tumor necrosis factors
diallyl sulfides
Modulation
secretion
Cytokines
interleukin-10
Derivatives
Interleukin-10
oils
dimethyl sulfide
Tumor Necrosis Factor-alpha
Garlic
interleukin-1
Interleukin-1

Keywords

  • Cytokine
  • Garlic
  • Inflammation
  • Nitric oxide
  • Organosulfur compounds

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

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title = "Modulation of cytokine secretion by garlic oil derivatives is associated with suppressed nitric oxide production in stimulated macrophages",
abstract = "We previously described that garlic oil derivatives differentially suppress the production of nitric oxide (NO) and prostaglandin E2 (PGE 2) in activated macrophages. In the present study, we investigated the effects of the garlic derivatives, diallyl sulfide (DAS), diallyl disulfide (DADS), and allyl methyl sulfide (AMS), on cytokine production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and the association between modulation of cytokines and inhibition of NO production was also assessed. The results indicated that these garlic compounds had different effects on the secretion of activated cytokines, including proinflammatory tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6, as well as the antiinflammatory, IL-10. DAS inhibited the production of all stimulated cytokines in a concentration-dependent manner, and the inhibition was closely associated with the suppression of NO and PGE2 production. DADS repressed the production of stimulated TNF-α and IL-10 and increased the production of activated IL-1β and, to a lesser extent, IL-6; but only the decreased IL-10 production was associated with DADS-induced NO inhibition. Yet, the DAS- and DADS-suppressed NO production was independent of TNF-α. AMS, on the other hand, slightly suppressed the stimulated TNF-α but enhanced IL-10 production, and such modulation was closely associated with the decrease in NO production.",
keywords = "Cytokine, Garlic, Inflammation, Nitric oxide, Organosulfur compounds",
author = "Chang, {Hsiao Pei} and Huang, {Shih Yi} and Chen, {Yue Hwa}",
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T1 - Modulation of cytokine secretion by garlic oil derivatives is associated with suppressed nitric oxide production in stimulated macrophages

AU - Chang, Hsiao Pei

AU - Huang, Shih Yi

AU - Chen, Yue Hwa

PY - 2005/4/6

Y1 - 2005/4/6

N2 - We previously described that garlic oil derivatives differentially suppress the production of nitric oxide (NO) and prostaglandin E2 (PGE 2) in activated macrophages. In the present study, we investigated the effects of the garlic derivatives, diallyl sulfide (DAS), diallyl disulfide (DADS), and allyl methyl sulfide (AMS), on cytokine production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and the association between modulation of cytokines and inhibition of NO production was also assessed. The results indicated that these garlic compounds had different effects on the secretion of activated cytokines, including proinflammatory tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6, as well as the antiinflammatory, IL-10. DAS inhibited the production of all stimulated cytokines in a concentration-dependent manner, and the inhibition was closely associated with the suppression of NO and PGE2 production. DADS repressed the production of stimulated TNF-α and IL-10 and increased the production of activated IL-1β and, to a lesser extent, IL-6; but only the decreased IL-10 production was associated with DADS-induced NO inhibition. Yet, the DAS- and DADS-suppressed NO production was independent of TNF-α. AMS, on the other hand, slightly suppressed the stimulated TNF-α but enhanced IL-10 production, and such modulation was closely associated with the decrease in NO production.

AB - We previously described that garlic oil derivatives differentially suppress the production of nitric oxide (NO) and prostaglandin E2 (PGE 2) in activated macrophages. In the present study, we investigated the effects of the garlic derivatives, diallyl sulfide (DAS), diallyl disulfide (DADS), and allyl methyl sulfide (AMS), on cytokine production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and the association between modulation of cytokines and inhibition of NO production was also assessed. The results indicated that these garlic compounds had different effects on the secretion of activated cytokines, including proinflammatory tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6, as well as the antiinflammatory, IL-10. DAS inhibited the production of all stimulated cytokines in a concentration-dependent manner, and the inhibition was closely associated with the suppression of NO and PGE2 production. DADS repressed the production of stimulated TNF-α and IL-10 and increased the production of activated IL-1β and, to a lesser extent, IL-6; but only the decreased IL-10 production was associated with DADS-induced NO inhibition. Yet, the DAS- and DADS-suppressed NO production was independent of TNF-α. AMS, on the other hand, slightly suppressed the stimulated TNF-α but enhanced IL-10 production, and such modulation was closely associated with the decrease in NO production.

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