Modulation of cell attachment and collagen production of anterior cruciate ligament cells via submicron grooves/ridges structures with different cell affinity

Peng Yuan Wang, Tsung Han Wu, Pen Hsiu Grace Chao, Wei Hsuan Kuo, Meng Jiy Wang, Cheng Che Hsu, Wei Bor Tsai

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This study aimed to investigate the effects of submicron-grooved topography and surface cell affinity on the attachment, proliferation and collagen synthesis of anterior cruciate ligament (ACL) cells. Two grooved polystyrene (PS) surfaces (equal groove/ridge width of 800nm) with a groove depth of 100 or 700nm were fabricated and modified by oxygen plasma treatment, dopamine deposition and conjugation of RGD-containing peptides to enhance cell affinity. The elongation and alignment of ACL cells was enhanced by grooved structures with increasing groove depths regardless of surface chemistry. On the other hand, cell spreading and proliferation mainly depended on surface chemistry, in accordance with surface cell affinity: O2 plasma<dopamine deposition<RGD conjugation. The synthesis of type I collagen was the highest by the ACL cells cultured on the 700nm grooved surface conjugated with RGD peptides, indicating that both surface grooved topography and chemistry play a role in modulating collagen production of ACL cells. Furthermore, the type I collagen deposited on the 700nm PS surface was aligned with grooves/ridges. Our results indicated that both ligand presentation and cell alignment are important in the physiological activities of ACL fibroblasts. Such information is critical for design of biomaterials for ACL tissue engineering.

Original languageEnglish
Pages (from-to)327-337
Number of pages11
JournalBiotechnology and Bioengineering
Volume110
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Anterior Cruciate Ligament
Ligaments
Collagen
Modulation
Surface chemistry
Polystyrenes
Collagen Type I
Peptides
Dopamine
Plasmas
Biocompatible Materials
Surface topography
Fibroblasts
Tissue engineering
Biomaterials
Topography
Tissue Engineering
Elongation
Ligands
Cultured Cells

Keywords

  • ACL fibroblasts
  • Alignment
  • Dopamine
  • RGD
  • Type I collagen

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Modulation of cell attachment and collagen production of anterior cruciate ligament cells via submicron grooves/ridges structures with different cell affinity. / Wang, Peng Yuan; Wu, Tsung Han; Chao, Pen Hsiu Grace; Kuo, Wei Hsuan; Wang, Meng Jiy; Hsu, Cheng Che; Tsai, Wei Bor.

In: Biotechnology and Bioengineering, Vol. 110, No. 1, 2013, p. 327-337.

Research output: Contribution to journalArticle

Wang, Peng Yuan ; Wu, Tsung Han ; Chao, Pen Hsiu Grace ; Kuo, Wei Hsuan ; Wang, Meng Jiy ; Hsu, Cheng Che ; Tsai, Wei Bor. / Modulation of cell attachment and collagen production of anterior cruciate ligament cells via submicron grooves/ridges structures with different cell affinity. In: Biotechnology and Bioengineering. 2013 ; Vol. 110, No. 1. pp. 327-337.
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