Modulation of alignment and differentiation of skeletal myoblasts by submicron ridges/grooves surface structure

Peng Yuan Wang, Hung Te Yu, Wei Bor Tsai

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Alignment and fusion of myoblasts into parallel arrays of multinucleated myotubes are critical in skeletal muscle tissue engineering. It is well known that contact guidance by grooves/ridges structures induces myoblasts to align and to migrate along the anisotropic direction. In this study, two series of grooved substrata with different widths (450 and 900 nm) and different depths (100, 350, and 550 nm) were studied on their effects on myoblast adhesion, proliferation, and differentiation into myotubes. We found that C2C12 cells were aligned and elongated along the direction of grooves. Groove depth was more influential on cellular morphology, proliferation, and differentiation than groove width. While cell proliferation was retarded on the grooved surfaces especially on the substrate with 900/550nm (width/depth), differentiation was also enhanced on the patterned surfaces compared to the flat control. Our results demonstrated the potential of grooved substrata with submicron scale in skeletal muscle tissue engineering.

Original languageEnglish
Pages (from-to)285-294
Number of pages10
JournalBiotechnology and Bioengineering
Volume106
Issue number2
DOIs
Publication statusPublished - Jun 1 2010
Externally publishedYes

Keywords

  • Alignment
  • C2C12 cells
  • C2C12 myoblasts
  • Differentiation
  • Grooved pattern
  • Grooves/ridges
  • Myotubes
  • Orientation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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