MMP-13 is involved in oral cancer cell metastasis

Shun Hong Huang, Ching Hsuan Law, Ping Hsueh Kuo, Ren Yu Hu, Ching Chieh Yang, Ting Wen Chung, Ji Min Li, Li Hsun Lin, Yi Chung Liu, En Chi Liao, Yi Ting Tsai, Yu Shan Wei, Chi Chen Lin, Chien Wen Chang, Hsiu Chuan Chou, Wen Ching Wang, Margaret Dah Tsyr Chang, Lu Hai Wang, Hsing Jien Kung, Hong Lin ChanPing Chiang Lyu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The oral cancer cell line OC3-I5 with a highly invasive ability was selected and derived from an established OSCC line OC3. In this study, we demonstrated that matrix metalloproteinases protein MMP-13 was up-regulated in OC3-I5 than in OC3 cells. We also observed that expression of epithelial-mesenchymal transition (EMT) markers including Twist, p-Src, Snail1, SIP1, JAM-A, and vinculin were increased in OC3-I5 compared to OC3 cells, whereas E-cadherin expression was decreased in the OC3-I5 cells. Using siMMP-13 knockdown techniques, we showed that siMMP-13 not only reduced the invasion and migration, but also the adhesion abilities of oral cancer cells. In support of the role of MMP-13 in metastasis, we used MMP-13 expressing plasmid-transfected 293T cells to enhance MMP-13 expression in the OC3 cells, transplanting the MMP-13 over expressing OC3 cells into nude mice led to enhanced lung metastasis. In summary, our findings show that MMP-13 promotes invasion and metastasis in oral cancer cells, suggesting altered expression of MMP-13 may be utilized to impede the process of metastasis.

Original languageEnglish
Pages (from-to)17144-17161
Number of pages18
JournalOncotarget
Volume7
Issue number13
DOIs
Publication statusPublished - Mar 29 2016
Externally publishedYes

Fingerprint

Mouth Neoplasms
Matrix Metalloproteinases
Neoplasm Metastasis
Vinculin
Epithelial-Mesenchymal Transition
HEK293 Cells
Cadherins
Nude Mice
Plasmids
Cell Line
Lung

Keywords

  • ECM
  • EMT
  • IF
  • MMP13
  • OSCC

ASJC Scopus subject areas

  • Oncology

Cite this

Huang, S. H., Law, C. H., Kuo, P. H., Hu, R. Y., Yang, C. C., Chung, T. W., ... Lyu, P. C. (2016). MMP-13 is involved in oral cancer cell metastasis. Oncotarget, 7(13), 17144-17161. https://doi.org/10.18632/oncotarget.7942

MMP-13 is involved in oral cancer cell metastasis. / Huang, Shun Hong; Law, Ching Hsuan; Kuo, Ping Hsueh; Hu, Ren Yu; Yang, Ching Chieh; Chung, Ting Wen; Li, Ji Min; Lin, Li Hsun; Liu, Yi Chung; Liao, En Chi; Tsai, Yi Ting; Wei, Yu Shan; Lin, Chi Chen; Chang, Chien Wen; Chou, Hsiu Chuan; Wang, Wen Ching; Chang, Margaret Dah Tsyr; Wang, Lu Hai; Kung, Hsing Jien; Chan, Hong Lin; Lyu, Ping Chiang.

In: Oncotarget, Vol. 7, No. 13, 29.03.2016, p. 17144-17161.

Research output: Contribution to journalArticle

Huang, SH, Law, CH, Kuo, PH, Hu, RY, Yang, CC, Chung, TW, Li, JM, Lin, LH, Liu, YC, Liao, EC, Tsai, YT, Wei, YS, Lin, CC, Chang, CW, Chou, HC, Wang, WC, Chang, MDT, Wang, LH, Kung, HJ, Chan, HL & Lyu, PC 2016, 'MMP-13 is involved in oral cancer cell metastasis', Oncotarget, vol. 7, no. 13, pp. 17144-17161. https://doi.org/10.18632/oncotarget.7942
Huang SH, Law CH, Kuo PH, Hu RY, Yang CC, Chung TW et al. MMP-13 is involved in oral cancer cell metastasis. Oncotarget. 2016 Mar 29;7(13):17144-17161. https://doi.org/10.18632/oncotarget.7942
Huang, Shun Hong ; Law, Ching Hsuan ; Kuo, Ping Hsueh ; Hu, Ren Yu ; Yang, Ching Chieh ; Chung, Ting Wen ; Li, Ji Min ; Lin, Li Hsun ; Liu, Yi Chung ; Liao, En Chi ; Tsai, Yi Ting ; Wei, Yu Shan ; Lin, Chi Chen ; Chang, Chien Wen ; Chou, Hsiu Chuan ; Wang, Wen Ching ; Chang, Margaret Dah Tsyr ; Wang, Lu Hai ; Kung, Hsing Jien ; Chan, Hong Lin ; Lyu, Ping Chiang. / MMP-13 is involved in oral cancer cell metastasis. In: Oncotarget. 2016 ; Vol. 7, No. 13. pp. 17144-17161.
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