MlcroRNA-124 inhibits proliferation and metastasis of esophageal cancer via negatively regulating NRP1

R. K. Zang, J. B. Ma, Y. C. Liang, Y. Wang, S. L. Hu, Y. Zhang, W. Dong, W. Zhang, L. K. Hu

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

OBJECTIVE: MicroRNAs are a kind of endogenous, non-coding RNAs, which exert a significant role in pathological processes. Previous studies have reported that microRNA- 124 is a tumor suppressor. The specific effect of microRNA-124 on esophageal cancer, however, has not been fully elucidated. This study aims to explore the role of microRNA-124 in esophageal cancer and its underlying mechanism. PATIENTS AND METHODS: MicroRNA-124 expressions in 75 esophageal cancer tissues, paracancerous tissues, and esophageal cancer cell lines were detected by qRT-PCR (quantitative Real-Time Polymerase Chain Reaction). The relationship between microRNA-124 expression, clinical progression, pathological indicators, and prognosis of patients with esophageal cancer was analyzed. For in vitro experiments, we performed CCK-8 (cell counting kit-8), colony formation and transwell assay to detect cell proliferation, migration, and invasion abilities after microRNA-124 overexpression in TE-1 and EC- 109 cells, respectively. Western blot was utilized to explore the regulatory role of microRNA-124 in esophageal cancer cells. RESULTS: MicroRNA-124 was downregulated in esophageal cancer tissues than that of paracancerous tissues. Patients with esophageal cancer who had lower expression level of microRNA-124 presented higher tumor stage and metastasis incidence, as well as lower survival rate. In vitro studies demonstrated a decreased cell proliferation and migration abilities after microRNA-124 overexpression. Western blot results showed upregulated PI3K and AKT, and downregulated PTEN in esophageal cancer cells after overexpression of microRNA-124. Furthermore, microRNA-124 was confirmed to negatively regulate NRP1, so as to participate in the development of esophageal cance r. CONCLUSIONS: MicroRNA-124 is downregulated in esophageal cancer tissues, which is remarkably correlated to the development, pathological grade, and poor prognosis of esophageal cancer. Overexpressed microRNA-124 is capable of inhibiting the malignant progression of esophageal cancer via negatively regulating NRP 1 .

Original languageEnglish
Pages (from-to)4532-4541
Number of pages10
JournalEuropean Review for Medical and Pharmacological Sciences
Volume22
Issue number14
Publication statusPublished - Jan 1 2018
Externally publishedYes

Fingerprint

Esophageal Neoplasms
MicroRNAs
Neoplasm Metastasis
Down-Regulation
Cell Movement
Western Blotting
Cell Proliferation
Untranslated RNA
Pathologic Processes
Phosphatidylinositol 3-Kinases
Real-Time Polymerase Chain Reaction
Neoplasms

Keywords

  • Esophageal cancer
  • Metastasis
  • MicroRNA-124
  • NRP1
  • Proliferation

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

MlcroRNA-124 inhibits proliferation and metastasis of esophageal cancer via negatively regulating NRP1. / Zang, R. K.; Ma, J. B.; Liang, Y. C.; Wang, Y.; Hu, S. L.; Zhang, Y.; Dong, W.; Zhang, W.; Hu, L. K.

In: European Review for Medical and Pharmacological Sciences, Vol. 22, No. 14, 01.01.2018, p. 4532-4541.

Research output: Contribution to journalReview article

Zang, RK, Ma, JB, Liang, YC, Wang, Y, Hu, SL, Zhang, Y, Dong, W, Zhang, W & Hu, LK 2018, 'MlcroRNA-124 inhibits proliferation and metastasis of esophageal cancer via negatively regulating NRP1', European Review for Medical and Pharmacological Sciences, vol. 22, no. 14, pp. 4532-4541.
Zang, R. K. ; Ma, J. B. ; Liang, Y. C. ; Wang, Y. ; Hu, S. L. ; Zhang, Y. ; Dong, W. ; Zhang, W. ; Hu, L. K. / MlcroRNA-124 inhibits proliferation and metastasis of esophageal cancer via negatively regulating NRP1. In: European Review for Medical and Pharmacological Sciences. 2018 ; Vol. 22, No. 14. pp. 4532-4541.
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abstract = "OBJECTIVE: MicroRNAs are a kind of endogenous, non-coding RNAs, which exert a significant role in pathological processes. Previous studies have reported that microRNA- 124 is a tumor suppressor. The specific effect of microRNA-124 on esophageal cancer, however, has not been fully elucidated. This study aims to explore the role of microRNA-124 in esophageal cancer and its underlying mechanism. PATIENTS AND METHODS: MicroRNA-124 expressions in 75 esophageal cancer tissues, paracancerous tissues, and esophageal cancer cell lines were detected by qRT-PCR (quantitative Real-Time Polymerase Chain Reaction). The relationship between microRNA-124 expression, clinical progression, pathological indicators, and prognosis of patients with esophageal cancer was analyzed. For in vitro experiments, we performed CCK-8 (cell counting kit-8), colony formation and transwell assay to detect cell proliferation, migration, and invasion abilities after microRNA-124 overexpression in TE-1 and EC- 109 cells, respectively. Western blot was utilized to explore the regulatory role of microRNA-124 in esophageal cancer cells. RESULTS: MicroRNA-124 was downregulated in esophageal cancer tissues than that of paracancerous tissues. Patients with esophageal cancer who had lower expression level of microRNA-124 presented higher tumor stage and metastasis incidence, as well as lower survival rate. In vitro studies demonstrated a decreased cell proliferation and migration abilities after microRNA-124 overexpression. Western blot results showed upregulated PI3K and AKT, and downregulated PTEN in esophageal cancer cells after overexpression of microRNA-124. Furthermore, microRNA-124 was confirmed to negatively regulate NRP1, so as to participate in the development of esophageal cance r. CONCLUSIONS: MicroRNA-124 is downregulated in esophageal cancer tissues, which is remarkably correlated to the development, pathological grade, and poor prognosis of esophageal cancer. Overexpressed microRNA-124 is capable of inhibiting the malignant progression of esophageal cancer via negatively regulating NRP 1 .",
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TY - JOUR

T1 - MlcroRNA-124 inhibits proliferation and metastasis of esophageal cancer via negatively regulating NRP1

AU - Zang, R. K.

AU - Ma, J. B.

AU - Liang, Y. C.

AU - Wang, Y.

AU - Hu, S. L.

AU - Zhang, Y.

AU - Dong, W.

AU - Zhang, W.

AU - Hu, L. K.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - OBJECTIVE: MicroRNAs are a kind of endogenous, non-coding RNAs, which exert a significant role in pathological processes. Previous studies have reported that microRNA- 124 is a tumor suppressor. The specific effect of microRNA-124 on esophageal cancer, however, has not been fully elucidated. This study aims to explore the role of microRNA-124 in esophageal cancer and its underlying mechanism. PATIENTS AND METHODS: MicroRNA-124 expressions in 75 esophageal cancer tissues, paracancerous tissues, and esophageal cancer cell lines were detected by qRT-PCR (quantitative Real-Time Polymerase Chain Reaction). The relationship between microRNA-124 expression, clinical progression, pathological indicators, and prognosis of patients with esophageal cancer was analyzed. For in vitro experiments, we performed CCK-8 (cell counting kit-8), colony formation and transwell assay to detect cell proliferation, migration, and invasion abilities after microRNA-124 overexpression in TE-1 and EC- 109 cells, respectively. Western blot was utilized to explore the regulatory role of microRNA-124 in esophageal cancer cells. RESULTS: MicroRNA-124 was downregulated in esophageal cancer tissues than that of paracancerous tissues. Patients with esophageal cancer who had lower expression level of microRNA-124 presented higher tumor stage and metastasis incidence, as well as lower survival rate. In vitro studies demonstrated a decreased cell proliferation and migration abilities after microRNA-124 overexpression. Western blot results showed upregulated PI3K and AKT, and downregulated PTEN in esophageal cancer cells after overexpression of microRNA-124. Furthermore, microRNA-124 was confirmed to negatively regulate NRP1, so as to participate in the development of esophageal cance r. CONCLUSIONS: MicroRNA-124 is downregulated in esophageal cancer tissues, which is remarkably correlated to the development, pathological grade, and poor prognosis of esophageal cancer. Overexpressed microRNA-124 is capable of inhibiting the malignant progression of esophageal cancer via negatively regulating NRP 1 .

AB - OBJECTIVE: MicroRNAs are a kind of endogenous, non-coding RNAs, which exert a significant role in pathological processes. Previous studies have reported that microRNA- 124 is a tumor suppressor. The specific effect of microRNA-124 on esophageal cancer, however, has not been fully elucidated. This study aims to explore the role of microRNA-124 in esophageal cancer and its underlying mechanism. PATIENTS AND METHODS: MicroRNA-124 expressions in 75 esophageal cancer tissues, paracancerous tissues, and esophageal cancer cell lines were detected by qRT-PCR (quantitative Real-Time Polymerase Chain Reaction). The relationship between microRNA-124 expression, clinical progression, pathological indicators, and prognosis of patients with esophageal cancer was analyzed. For in vitro experiments, we performed CCK-8 (cell counting kit-8), colony formation and transwell assay to detect cell proliferation, migration, and invasion abilities after microRNA-124 overexpression in TE-1 and EC- 109 cells, respectively. Western blot was utilized to explore the regulatory role of microRNA-124 in esophageal cancer cells. RESULTS: MicroRNA-124 was downregulated in esophageal cancer tissues than that of paracancerous tissues. Patients with esophageal cancer who had lower expression level of microRNA-124 presented higher tumor stage and metastasis incidence, as well as lower survival rate. In vitro studies demonstrated a decreased cell proliferation and migration abilities after microRNA-124 overexpression. Western blot results showed upregulated PI3K and AKT, and downregulated PTEN in esophageal cancer cells after overexpression of microRNA-124. Furthermore, microRNA-124 was confirmed to negatively regulate NRP1, so as to participate in the development of esophageal cance r. CONCLUSIONS: MicroRNA-124 is downregulated in esophageal cancer tissues, which is remarkably correlated to the development, pathological grade, and poor prognosis of esophageal cancer. Overexpressed microRNA-124 is capable of inhibiting the malignant progression of esophageal cancer via negatively regulating NRP 1 .

KW - Esophageal cancer

KW - Metastasis

KW - MicroRNA-124

KW - NRP1

KW - Proliferation

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