Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan

Chih Ho Lai, Ju Chun Huang, Chuan Chiang-Ni, Ju Pi Li, Lii Tzu Wu, Hua Shan Wu, Yu Chen Sun, Mei Ling Lin, Ju Fang Lee, Hwai Jeng Lin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background. Persistent Helicobacter pylori infection may induce several upper gastrointestinal diseases. Two major virulence factors of H. pylori, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), are thought to be associated with the severity of disease progression. The distribution of vacA and cag-pathogenicity island (cag-PAI) alleles varies in H. pylori isolated from patients in different geographic regions. Aim. To assess the association between mixed infection of H. pylori clinical isolates from Taiwanese patients and the severity of gastrointestinal diseases. Methods. A total of 70 patients were enrolled in this study. Six distinct and well-separated colonies were isolated from each patient and 420 colonies were analyzed to determine the genotypes of virulence genes. Results. The prevalence of mixed infections of all H. pylori-infected patients was 28.6% (20/70). The rate of mixed infections in patients with duodenal ulcer (47.6%) was much higher than that with other gastrointestinal diseases (P < 0.05). Conclusions. H. pylori mixed infections show high genetic diversity that may enhance bacterial adaptation to the hostile environment of the stomach and contribute to disease development.

Original languageEnglish
Article number7521913
JournalGastroenterology Research and Practice
Volume2016
DOIs
Publication statusPublished - 2016

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Gastrointestinal Diseases
Coinfection
Taiwan
Helicobacter pylori
Genomic Islands
Complement Factor H
Cytotoxins
Helicobacter Infections
Virulence Factors
Duodenal Ulcer
Genes
Virulence
Disease Progression
Stomach
Alleles
Genotype

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan. / Lai, Chih Ho; Huang, Ju Chun; Chiang-Ni, Chuan; Li, Ju Pi; Wu, Lii Tzu; Wu, Hua Shan; Sun, Yu Chen; Lin, Mei Ling; Lee, Ju Fang; Lin, Hwai Jeng.

In: Gastroenterology Research and Practice, Vol. 2016, 7521913, 2016.

Research output: Contribution to journalArticle

Lai, Chih Ho ; Huang, Ju Chun ; Chiang-Ni, Chuan ; Li, Ju Pi ; Wu, Lii Tzu ; Wu, Hua Shan ; Sun, Yu Chen ; Lin, Mei Ling ; Lee, Ju Fang ; Lin, Hwai Jeng. / Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan. In: Gastroenterology Research and Practice. 2016 ; Vol. 2016.
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abstract = "Background. Persistent Helicobacter pylori infection may induce several upper gastrointestinal diseases. Two major virulence factors of H. pylori, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), are thought to be associated with the severity of disease progression. The distribution of vacA and cag-pathogenicity island (cag-PAI) alleles varies in H. pylori isolated from patients in different geographic regions. Aim. To assess the association between mixed infection of H. pylori clinical isolates from Taiwanese patients and the severity of gastrointestinal diseases. Methods. A total of 70 patients were enrolled in this study. Six distinct and well-separated colonies were isolated from each patient and 420 colonies were analyzed to determine the genotypes of virulence genes. Results. The prevalence of mixed infections of all H. pylori-infected patients was 28.6{\%} (20/70). The rate of mixed infections in patients with duodenal ulcer (47.6{\%}) was much higher than that with other gastrointestinal diseases (P < 0.05). Conclusions. H. pylori mixed infections show high genetic diversity that may enhance bacterial adaptation to the hostile environment of the stomach and contribute to disease development.",
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AU - Wu, Hua Shan

AU - Sun, Yu Chen

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