Mitochondrial dynamics in Angiostrongylus cantonensis-infected mouse brain

Ho Yin Pekkle Lam, Cheng Chi Chen, Tina Tu Wen Chen, Kai Chih Chang, Wen Jui Wu, Ting Hua Yang, Ting Ruei Liang, Po Ching Cheng, Shih Yi Peng

Research output: Contribution to journalArticlepeer-review

Abstract

Angiostrongylus cantonensis is one of the most widespread parasites causing central nervous system (CNS) diseases in mammals. Since the mitochondrion is an essential cell organelle responsible for both physiological and pathological processes, its dysfunction might lead to inflammation and multiple disorders. In this study we aimed to investigate the changes in mitochondrial dynamics that occur in the mouse brain upon infection with A. cantonensis, using molecular biology techniques such as polymerase chain reaction (PCR), western blot analysis, transmission electron microscopy (TEM), and different staining methods. Here, we show that mouse brain infected with A. cantonensis exhibits altered mitochondrial dynamics, including fission, fusion, and biogenesis. Additionally, we demonstrate that caspases and B-cell lymphoma 2 (BCL-2) were significantly upregulated in A. cantonensis-infected brain. These results are indicative of the occurrence of apoptosis during A. cantonensis infection, which was further confirmed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. These findings suggest the change in mitochondrial dynamics in A. cantonensis-infected brain, providing another point of view on the pathogenesis of meningoencephalitis caused by A. cantonensis infection.

Original languageEnglish
Article number102231
JournalParasitology International
Volume80
DOIs
Publication statusPublished - Feb 2021

Keywords

  • Angiostrongylus
  • Mitochondria
  • Mitochondrial dynamics

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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