Mitochondrial alterations of skeletal muscle in a heat stress rat model.

Y. D. Hsu, S. S. Chen, W. H. Lee, S. Z. Lin, M. C. Kao, W. L. Tsao

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Abstract

The morphological and ultrastructural alterations of skeletal muscle in experimental rats with heat stress were investigated. Fifteen male Sprague-Dawley rats were exposed in a 42 degrees C constant temperature oven, resulting in a heat stress state; ten rats were used as controls. All treated rats had weakness of the 4 limbs associated with increased serum creatine kinase levels (p <0.01). Soleus muscles were submitted to histological, histochemical, ultrastructural and quantitative-morphometric analysis. The group receiving heat stress showed many ragged-red fibers in Gomori trichrome stain and appeared hyper-reactive in succinate dehydrogenase and cytochrome C oxidase stains. The ultrastructure of ragged-red fibers showed increased mitochondrial aggregation as multiple small nests, which were particularly located in the subsarcolemmal space. The mitochondrial area was significantly increased in heat stress rats (p <0.001). The consistently increased mitochondrial area and histochemical alterations of mitochondria are early pathological abnormalities in muscles with heat stress and indicate fundamental impairment of energy metabolism.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalProceedings of the National Science Council, Republic of China. Part B, Life sciences
Volume19
Issue number4
Publication statusPublished - Oct 1995
Externally publishedYes

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Skeletal Muscle
Hot Temperature
Succinate Dehydrogenase
Electron Transport Complex IV
Creatine Kinase
Energy Metabolism
Sprague Dawley Rats
Mitochondria
Coloring Agents
Extremities
Muscles
Temperature
Serum

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Mitochondrial alterations of skeletal muscle in a heat stress rat model. / Hsu, Y. D.; Chen, S. S.; Lee, W. H.; Lin, S. Z.; Kao, M. C.; Tsao, W. L.

In: Proceedings of the National Science Council, Republic of China. Part B, Life sciences, Vol. 19, No. 4, 10.1995, p. 233-239.

Research output: Contribution to journalArticle

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