MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis

Chueh Wei Wu, Ya Wen Cheng, Nan Yung Hsu, Ken Tu Yeh, Yi Yu Tsai, Chun Chi Chiang, Wei Ran Wang, Jai Nien Tung

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Purpose: MiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRNA, miRNA-221, and downstream p27Kip1 gene expression are correlated with the pathogenesis of pterygium. Methods: We collected 120 pterygial and 120 normal conjunctival samples for this study. Immunohistochemistry and real-time reverse transcription (RT)-PCR were performed to determine β-catenin protein localization, miR-221, and p27Kip1 gene expression. Pterygium cell line (PECs) cell models were used to confirm the effect of β-catenin, miR-221, and p27Kip1 gene in the proliferation of pterygium cells. Results: Seventy-two (60.0%) pterygial specimens showed high miR-221 expression levels, which was significantly higher than the control groups (13 of 120, 10.8%, p<0.0001). MiR-221 expression was significantly higher in β-catenin-nuclear/cytoplasmic-positive groups than in β-catenin membrane-positive and negative groups (p=0.001). We also found that p27Kip1 gene expression in pterygium was negatively correlated with miR-221 expression (p=0.002). In the clinical association, miR-221 expression was significantly higher in the fleshy and intermediate groups than in the atrophic group (p=0.007). The association of miR-221, p27Kip1 and proliferation of pterygium were also confirmed in the PECs model. Conclusions: Our study demonstrated that activation of β-catenin in pterygium may interact with miR-221, resulting in p27Kip1 gene downregulation that influences pterygium pathogenesis. © 2014 Molecular Vision.

Original languageEnglish
Pages (from-to)1048-1056
Number of pages9
JournalMolecular Vision
Volume20
Publication statusPublished - Jul 18 2014

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Pterygium
Catenins
Gene Expression
MicroRNAs
Small Untranslated RNA
RNA Stability
Genes
Reverse Transcription
Down-Regulation
Immunohistochemistry
Cell Proliferation
Cell Line
Polymerase Chain Reaction
Control Groups
Membranes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Wu, C. W., Cheng, Y. W., Hsu, N. Y., Yeh, K. T., Tsai, Y. Y., Chiang, C. C., ... Tung, J. N. (2014). MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis. Molecular Vision, 20, 1048-1056.

MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis. / Wu, Chueh Wei; Cheng, Ya Wen; Hsu, Nan Yung; Yeh, Ken Tu; Tsai, Yi Yu; Chiang, Chun Chi; Wang, Wei Ran; Tung, Jai Nien.

In: Molecular Vision, Vol. 20, 18.07.2014, p. 1048-1056.

Research output: Contribution to journalArticle

Wu, CW, Cheng, YW, Hsu, NY, Yeh, KT, Tsai, YY, Chiang, CC, Wang, WR & Tung, JN 2014, 'MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis', Molecular Vision, vol. 20, pp. 1048-1056.
Wu CW, Cheng YW, Hsu NY, Yeh KT, Tsai YY, Chiang CC et al. MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis. Molecular Vision. 2014 Jul 18;20:1048-1056.
Wu, Chueh Wei ; Cheng, Ya Wen ; Hsu, Nan Yung ; Yeh, Ken Tu ; Tsai, Yi Yu ; Chiang, Chun Chi ; Wang, Wei Ran ; Tung, Jai Nien. / MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis. In: Molecular Vision. 2014 ; Vol. 20. pp. 1048-1056.
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abstract = "Purpose: MiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRNA, miRNA-221, and downstream p27Kip1 gene expression are correlated with the pathogenesis of pterygium. Methods: We collected 120 pterygial and 120 normal conjunctival samples for this study. Immunohistochemistry and real-time reverse transcription (RT)-PCR were performed to determine β-catenin protein localization, miR-221, and p27Kip1 gene expression. Pterygium cell line (PECs) cell models were used to confirm the effect of β-catenin, miR-221, and p27Kip1 gene in the proliferation of pterygium cells. Results: Seventy-two (60.0{\%}) pterygial specimens showed high miR-221 expression levels, which was significantly higher than the control groups (13 of 120, 10.8{\%}, p<0.0001). MiR-221 expression was significantly higher in β-catenin-nuclear/cytoplasmic-positive groups than in β-catenin membrane-positive and negative groups (p=0.001). We also found that p27Kip1 gene expression in pterygium was negatively correlated with miR-221 expression (p=0.002). In the clinical association, miR-221 expression was significantly higher in the fleshy and intermediate groups than in the atrophic group (p=0.007). The association of miR-221, p27Kip1 and proliferation of pterygium were also confirmed in the PECs model. Conclusions: Our study demonstrated that activation of β-catenin in pterygium may interact with miR-221, resulting in p27Kip1 gene downregulation that influences pterygium pathogenesis. {\circledC} 2014 Molecular Vision.",
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AU - Wu, Chueh Wei

AU - Cheng, Ya Wen

AU - Hsu, Nan Yung

AU - Yeh, Ken Tu

AU - Tsai, Yi Yu

AU - Chiang, Chun Chi

AU - Wang, Wei Ran

AU - Tung, Jai Nien

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N2 - Purpose: MiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRNA, miRNA-221, and downstream p27Kip1 gene expression are correlated with the pathogenesis of pterygium. Methods: We collected 120 pterygial and 120 normal conjunctival samples for this study. Immunohistochemistry and real-time reverse transcription (RT)-PCR were performed to determine β-catenin protein localization, miR-221, and p27Kip1 gene expression. Pterygium cell line (PECs) cell models were used to confirm the effect of β-catenin, miR-221, and p27Kip1 gene in the proliferation of pterygium cells. Results: Seventy-two (60.0%) pterygial specimens showed high miR-221 expression levels, which was significantly higher than the control groups (13 of 120, 10.8%, p<0.0001). MiR-221 expression was significantly higher in β-catenin-nuclear/cytoplasmic-positive groups than in β-catenin membrane-positive and negative groups (p=0.001). We also found that p27Kip1 gene expression in pterygium was negatively correlated with miR-221 expression (p=0.002). In the clinical association, miR-221 expression was significantly higher in the fleshy and intermediate groups than in the atrophic group (p=0.007). The association of miR-221, p27Kip1 and proliferation of pterygium were also confirmed in the PECs model. Conclusions: Our study demonstrated that activation of β-catenin in pterygium may interact with miR-221, resulting in p27Kip1 gene downregulation that influences pterygium pathogenesis. © 2014 Molecular Vision.

AB - Purpose: MiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRNA, miRNA-221, and downstream p27Kip1 gene expression are correlated with the pathogenesis of pterygium. Methods: We collected 120 pterygial and 120 normal conjunctival samples for this study. Immunohistochemistry and real-time reverse transcription (RT)-PCR were performed to determine β-catenin protein localization, miR-221, and p27Kip1 gene expression. Pterygium cell line (PECs) cell models were used to confirm the effect of β-catenin, miR-221, and p27Kip1 gene in the proliferation of pterygium cells. Results: Seventy-two (60.0%) pterygial specimens showed high miR-221 expression levels, which was significantly higher than the control groups (13 of 120, 10.8%, p<0.0001). MiR-221 expression was significantly higher in β-catenin-nuclear/cytoplasmic-positive groups than in β-catenin membrane-positive and negative groups (p=0.001). We also found that p27Kip1 gene expression in pterygium was negatively correlated with miR-221 expression (p=0.002). In the clinical association, miR-221 expression was significantly higher in the fleshy and intermediate groups than in the atrophic group (p=0.007). The association of miR-221, p27Kip1 and proliferation of pterygium were also confirmed in the PECs model. Conclusions: Our study demonstrated that activation of β-catenin in pterygium may interact with miR-221, resulting in p27Kip1 gene downregulation that influences pterygium pathogenesis. © 2014 Molecular Vision.

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