Minocycline attenuates experimental colitis in mice by blocking expression of inducible nitric oxide synthase and matrix metalloproteinases

Tien-Yu Huang, Heng-Cheng Chu, Yi-Ling Lin, Chih-Kung Lin, Tsai-Yuan Hsieh, Wei-Kuo Chang, You-Chen Chao, Ching-Len Liao

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In addition to its antimicrobial activity, minocycline exerts anti-inflammatory effects in several disease models. However, whether minocycline affects the pathogenesis of inflammatory bowel disease has not been determined. We investigated the effects of minocycline on experimental colitis and its underlying mechanisms. Acute and chronic colitis were induced in mice by treatment with dextran sulfate sodium (DSS) or trinitrobenzene sulfonic acid (TNBS), and the effect of minocycline on colonic injury was assessed clinically and histologically. Prophylactic and therapeutic treatment of mice with minocycline significantly diminished mortality rate and attenuated the severity of DSS-induced acute colitis. Mechanistically, minocycline administration suppressed inducible nitric oxide synthase (iNOS) expression and nitrotyrosine production, inhibited proinflammatory cytokine expression, repressed the elevated mRNA expression of matrix metalloproteinases (MMPs) 2, 3, 9, and 13, diminished the apoptotic index in colonic tissues, and inhibited nitric oxide production in the serum of mice with DSS-induced acute colitis. In DSS-induced chronic colitis, minocycline treatment also reduced body weight loss, improved colonic histology, and blocked expression of iNOS, proinflammatory cytokines, and MMPs from colonic tissues. Similarly, minocycline could ameliorate the severity of TNBS-induced acute colitis in mice by decreasing mortality rate and inhibiting proinflammatory cytokine expression in colonic tissues. These results demonstrate that minocycline protects mice against DSS- and TNBS-induced colitis, probably via inhibition of iNOS and MMP expression in intestinal tissues. Therefore, minocycline is a potential remedy for human inflammatory bowel diseases. © 2009 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)69-82
Number of pages14
JournalToxicology and Applied Pharmacology
Volume237
Issue number1
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Colitis
  • Dextran sulfate sodium
  • Inducible nitric oxide synthase
  • Inflammatory bowel diseases
  • Matrix metalloproteinases
  • Minocycline
  • Trinitrobenzene sulfonic acid
  • antibiotic agent
  • collagenase 3
  • dextran sulfate
  • gelatinase A
  • gelatinase B
  • inducible nitric oxide synthase
  • matrix metalloproteinase
  • messenger RNA
  • minocycline
  • nitric oxide
  • stromelysin
  • trinitrobenzenesulfonic acid
  • acute disease
  • animal experiment
  • animal model
  • antiinflammatory activity
  • article
  • chronic disease
  • colitis
  • controlled study
  • disease severity
  • drug dose comparison
  • drug dose increase
  • enteritis
  • experimental model
  • immune response
  • immunohistochemistry
  • intestine cell
  • male
  • mortality
  • mouse
  • nonhuman
  • real time polymerase chain reaction
  • reverse transcription polymerase chain reaction
  • Animals
  • Anti-Bacterial Agents
  • Anti-Inflammatory Agents
  • Cytokines
  • Dextran Sulfate
  • Disease Models, Animal
  • Enzyme Inhibitors
  • Inflammation
  • Male
  • Matrix Metalloproteinases
  • Metronidazole
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Nitric Oxide Synthase Type II
  • RNA, Messenger
  • Species Specificity
  • Survival Analysis
  • Trinitrobenzenesulfonic Acid
  • Mus

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