Middle Infrared Radiation Induces G2/M Cell Cycle Arrest in A549 Lung Cancer Cells

Hsin Yi Chang, Meng Her Shih, Hsuan Cheng Huang, Shang Ru Tsai, Hsueh Fen Juan, Si Chen Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

There were studies investigating the effects of broadband infrared radiation (IR) on cancer cell, while the influences of middle-infrared radiation (MIR) are still unknown. In this study, a MIR emitter with emission wavelength band in the 3-5 μm region was developed to irradiate A549 lung adenocarcinoma cells. It was found that MIR exposure inhibited cell proliferation and induced morphological changes by altering the cellular distribution of cytoskeletal components. Using quantitative PCR, we found that MIR promoted the expression levels of ATM (ataxia telangiectasia mutated), ATR (ataxia-telangiectasia and Rad3-related and Rad3-related), TP53 (tumor protein p53), p21 (CDKN1A, cyclin-dependent kinase inhibitor 1A) and GADD45 (growth arrest and DNA-damage inducible), but decreased the expression levels of cyclin B coding genes, CCNB1 and CCNB2, as well as CDK1 (Cyclin-dependent kinase 1). The reduction of protein expression levels of CDC25C, cyclin B1 and the phosphorylation of CDK1 at Thr-161 altogether suggest G2/M arrest occurred in A549 cells by MIR. DNA repair foci formation of DNA double-strand breaks (DSB) marker γ-H2AX and sensor 53BP1 was induced by MIR treatment, it implies the MIR induced G2/M cell cycle arrest resulted from DSB. This study illustrates a potential role for the use of MIR in lung cancer therapy by initiating DSB and blocking cell cycle progression.

Original languageEnglish
Article numbere54117
JournalPLoS One
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 15 2013
Externally publishedYes

Fingerprint

G2 Phase Cell Cycle Checkpoints
infrared radiation
lung neoplasms
Lung Neoplasms
Cells
Radiation
Infrared radiation
cyclin-dependent kinase
CDC2 Protein Kinase
Ataxia Telangiectasia
cyclins
DNA
Cyclin-Dependent Kinase Inhibitor p21
Cyclin B
Cyclin B1
Double-Stranded DNA Breaks
cell cycle checkpoints
neoplasm cells
Phosphorylation
Cell proliferation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Middle Infrared Radiation Induces G2/M Cell Cycle Arrest in A549 Lung Cancer Cells. / Chang, Hsin Yi; Shih, Meng Her; Huang, Hsuan Cheng; Tsai, Shang Ru; Juan, Hsueh Fen; Lee, Si Chen.

In: PLoS One, Vol. 8, No. 1, e54117, 15.01.2013.

Research output: Contribution to journalArticle

Chang, Hsin Yi ; Shih, Meng Her ; Huang, Hsuan Cheng ; Tsai, Shang Ru ; Juan, Hsueh Fen ; Lee, Si Chen. / Middle Infrared Radiation Induces G2/M Cell Cycle Arrest in A549 Lung Cancer Cells. In: PLoS One. 2013 ; Vol. 8, No. 1.
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