Microvascular effects of Photofrin®-induced photodynamic therapy

Cheng Jen Chang, Sally M.H. Cheng, J. Stuart Nelson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background and objective: The object of our study is to evaluate the feasibility of photodynamic therapy (PDT) for complicated hemangiomas. The photosensitizing activities of Photofrin® have been used in vivo models for our goal of evaluation. Study design/materials and methods: The in vivo biological activities of Photofrin® exposed to the total laser energy density of 100 J/cm2 with the power density of 100 or 120 mW/cm2 at 630 nm wavelength was studied. The amount of vascular damage produced in the chick chorioallantoic membrane (CAM) was evaluated. At 630 nm wavelength, those individual vessels with a diameter of 40 μm or less and those with a diameter between 40 and 100 μm were treated with Photofrin® at a concentration of about 2.5 mg/mL, and injected intraperitoneally at 2.5 mg/kg, illuminated at 100 and 120 mW/cm2, respectively. Both exhibited coagulation. Results: There were no statistically significant differences between the two groups (100 and 120 mW/cm2) on vessel damage grade 1. With vessel damage grades 2 and 3, the differences were statistically significant between two groups. Vessel damages between arterioles and venules also demonstrated differences in the 100 mW/cm2 treated group but not in the 120 mW/cm2 group. Statistically significant differences were also shown in arteriole and venules damage between 100 and 120 mW/cm2 treated groups. The severity of vessel damage between grades 1 and 2, 1 and 3, and 2 and 3, were compared. The differences were statistically significant in 100 mW/cm2 treated group. There was no statistically significant difference in 120 mW/cm2 treated group. Conclusion: Photofrin® has the capabilities for destruction of microvascular vessels of CAM. Extension of this study to the second-generation photosensitizers is underway. The most important treatment variables seem to be the power density.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalPhotodiagnosis and Photodynamic Therapy
Volume4
Issue number2
DOIs
Publication statusPublished - Jun 1 2007
Externally publishedYes

Keywords

  • Chick chorioallantoic membrane
  • Photodynamic therapy
  • Photofrin

ASJC Scopus subject areas

  • Biophysics
  • Oncology
  • Dermatology
  • Pharmacology (medical)

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