Microsatellite instability in sporadic-colon-cancer patients with and without liver metastases

Wei Shone Chen, Jeou Yuan Chen, Jacqueline Ming Liu, Wen Chang Lin, Kuang Liang King, Jacqueline Whang-Peng, Wen Kuang Yang

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Microsatellite instability (MSI) is intrinsic to most colorectal carcinomas (CRC) from patients with hereditary non-polyposis colorectal cancer (HNPCC), reflecting germline mutations in the mismatch-repair (MMR) genes. Its occurrence and chronological sequence of development in sporadic CRC appears less well defined. To explore the time sequence in acquisition of MSI, and the role it plays during tumor progression in sporadic CRC, we compared the incidence of MSI in tissue samples from 40 Dukes'-B and 30 Dukes'-D CRC patients with liver metastases, at 4 different microsatellite loci, representing sites on the APC, DCC and p53 genes respectively as well as the D2S 123 site. Among the 30 patients with hepatic metastases, MSI was found in 9 (30%) of the primary, and 13 (43.3%) of the metastatic tumors. In comparison, among the 40 Dukes'-B CRC, MSI was found in only 8 cases (20%). CRC with MSI were more frequently located in the right colon, less frequently on the left side, and seldom in the rectum. Tumor ploidy analysis shows that 46.2% of Dukes'-D primary tumors with MSl are diploid (χ2 = 4.46, p = 0.035). With a mean follow-up time of 4.2 years for the Dukes'-B CRC, there were no recurrences in the 8 patients with MSI, whilst 6 (18.8%) relapses occurred amongst the 32 patients without MSI, average time to recurrence being IS months. In Dukes'-D CRC, mean survival time for patients with MSI was 37 months (95% CI, 24 to 51 months), for those without MSI 26 months (95% CI; 18 to 35 months), although this was not statistically significant. Our data suggest that tumor progression may involve increased genetic instability.

Original languageEnglish
Pages (from-to)470-474
Number of pages5
JournalInternational Journal of Cancer
Volume74
Issue number4
DOIs
Publication statusPublished - Oct 2 1997
Externally publishedYes

Fingerprint

Microsatellite Instability
Colonic Neoplasms
Colorectal Neoplasms
Neoplasm Metastasis
Liver
Neoplasms
Recurrence
DCC Genes
APC Genes
DNA Mismatch Repair
Germ-Line Mutation
Ploidies
p53 Genes
Diploidy
Rectum
Microsatellite Repeats
Colon
Survival Rate

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Microsatellite instability in sporadic-colon-cancer patients with and without liver metastases. / Chen, Wei Shone; Chen, Jeou Yuan; Liu, Jacqueline Ming; Lin, Wen Chang; King, Kuang Liang; Whang-Peng, Jacqueline; Yang, Wen Kuang.

In: International Journal of Cancer, Vol. 74, No. 4, 02.10.1997, p. 470-474.

Research output: Contribution to journalArticle

Chen, Wei Shone ; Chen, Jeou Yuan ; Liu, Jacqueline Ming ; Lin, Wen Chang ; King, Kuang Liang ; Whang-Peng, Jacqueline ; Yang, Wen Kuang. / Microsatellite instability in sporadic-colon-cancer patients with and without liver metastases. In: International Journal of Cancer. 1997 ; Vol. 74, No. 4. pp. 470-474.
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abstract = "Microsatellite instability (MSI) is intrinsic to most colorectal carcinomas (CRC) from patients with hereditary non-polyposis colorectal cancer (HNPCC), reflecting germline mutations in the mismatch-repair (MMR) genes. Its occurrence and chronological sequence of development in sporadic CRC appears less well defined. To explore the time sequence in acquisition of MSI, and the role it plays during tumor progression in sporadic CRC, we compared the incidence of MSI in tissue samples from 40 Dukes'-B and 30 Dukes'-D CRC patients with liver metastases, at 4 different microsatellite loci, representing sites on the APC, DCC and p53 genes respectively as well as the D2S 123 site. Among the 30 patients with hepatic metastases, MSI was found in 9 (30{\%}) of the primary, and 13 (43.3{\%}) of the metastatic tumors. In comparison, among the 40 Dukes'-B CRC, MSI was found in only 8 cases (20{\%}). CRC with MSI were more frequently located in the right colon, less frequently on the left side, and seldom in the rectum. Tumor ploidy analysis shows that 46.2{\%} of Dukes'-D primary tumors with MSl are diploid (χ2 = 4.46, p = 0.035). With a mean follow-up time of 4.2 years for the Dukes'-B CRC, there were no recurrences in the 8 patients with MSI, whilst 6 (18.8{\%}) relapses occurred amongst the 32 patients without MSI, average time to recurrence being IS months. In Dukes'-D CRC, mean survival time for patients with MSI was 37 months (95{\%} CI, 24 to 51 months), for those without MSI 26 months (95{\%} CI; 18 to 35 months), although this was not statistically significant. Our data suggest that tumor progression may involve increased genetic instability.",
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