Abstract
| Original language | English |
|---|---|
| Journal | PLoS One |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2012 |
| Externally published | Yes |
Fingerprint
Keywords
- androgen
- lentivirus vector
- microRNA
- microRNA let 7c
- regulator protein
- tumor suppressor protein
- unclassified drug
- green fluorescent protein
- mirnlet7 microRNA, human
- animal cell
- animal experiment
- animal tissue
- article
- cancer cell culture
- cancer growth
- cancer inhibition
- castration resistant prostate cancer
- cell growth
- cell proliferation
- clonogenesis
- controlled study
- down regulation
- drug targeting
- gene overexpression
- human
- human cell
- in situ hybridization
- in vitro study
- male
- mouse
- nonhuman
- protein expression
- protein function
- reverse transcription polymerase chain reaction
- RNA processing
- tumor volume
- tumor xenograft
- upregulation
- animal
- experimental neoplasm
- gene expression regulation
- genetics
- Lentivirinae
- metabolism
- Northern blotting
- nude mouse
- orchiectomy
- pathology
- prostate tumor
- tumor cell line
- tumor suppressor gene
- xenograft
- Androgens
- Animals
- Blotting, Northern
- Cell Line, Tumor
- Cell Proliferation
- Down-Regulation
- Gene Expression Regulation, Neoplastic
- Genes, Tumor Suppressor
- Green Fluorescent Proteins
- Humans
- In Situ Hybridization
- Lentivirus
- Male
- Mice
- Mice, Nude
- MicroRNAs
- Neoplasms, Experimental
- Orchiectomy
- Prostatic Neoplasms
- Reverse Transcriptase Polymerase Chain Reaction
- Transplantation, Heterologous
- Tumor Burden
Cite this
MicroRNA let-7c is downregulated in prostate cancer and suppresses prostate cancer growth. / Nadiminty, N.; Tummala, R.; Lou, W.; Zhu, Y.; Shi, X.-B.; Zou, J.X.; Chen, H.; Zhang, J.; Chen, X.; Luo, J.; deVere White, R.W.; Kung, H.-J.; Evans, C.P.; Gao, A.C.
In: PLoS One, Vol. 7, No. 3, 03.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MicroRNA let-7c is downregulated in prostate cancer and suppresses prostate cancer growth
AU - Nadiminty, N.
AU - Tummala, R.
AU - Lou, W.
AU - Zhu, Y.
AU - Shi, X.-B.
AU - Zou, J.X.
AU - Chen, H.
AU - Zhang, J.
AU - Chen, X.
AU - Luo, J.
AU - deVere White, R.W.
AU - Kung, H.-J.
AU - Evans, C.P.
AU - Gao, A.C.
N1 - 引用次數:82 Export Date: 5 March 2018 通訊地址: Nadiminty, N.; Department of Urology, University of California Davis, Sacramento, CA, United States; 電子郵件: acgao@ucdavis.edu 化學物質/CAS: Androgens; Green Fluorescent Proteins, 147336-22-9; MicroRNAs; mirnlet7 microRNA, human 參考文獻: Shi, X.-B., Tepper, C.G., deVere White, R.W., Cancerous miRNAs and their regulation Cell Cycle (2008), 7, pp. 1529-1538; Coppola, V., de Maria, R., Bonci, D., (2009) MicroRNAs and prostate cancer, pp. ER0170-ER0172. , Endocr Relat Cancer; Gandellini, P., Folini, M., Zaffaroni, N., Towards the definition of prostate cancer-related microRNAs: where are we now? 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PY - 2012/3
Y1 - 2012/3
N2 - Purpose: Prostate cancer (PCa) is characterized by deregulated expression of several tumor suppressor or oncogenic miRNAs. The objective of this study was the identification and characterization of miR-let-7c as a potential tumor suppressor in PCa. Experimental Design: Levels of expression of miR-let-7c were examined in human PCa cell lines and tissues using qRT-PCR and in situ hybridization. Let-7c was overexpressed or suppressed to assess the effects on the growth of human PCa cell lines. Lentiviral-mediated re-expression of let-7c was utilized to assess the effects on human PCa xenografts. Results: We identified miR-let-7c as a potential tumor suppressor in PCa. Expression of let-7c is downregulated in castration-resistant prostate cancer (CRPC) cells. Overexpression of let-7c decreased while downregulation of let-7c increased cell proliferation, clonogenicity and anchorage-independent growth of PCa cells in vitro. Suppression of let-7c expression enhanced the ability of androgen-sensitive PCa cells to grow in androgen-deprived conditions in vitro. Reconstitution of Let-7c by lentiviral-mediated intratumoral delivery significantly reduced tumor burden in xenografts of human PCa cells. Furthermore, let-7c expression is downregulated in clinical PCa specimens compared to their matched benign tissues, while the expression of Lin28, a master regulator of let-7 miRNA processing, is upregulated in clinical PCa specimens. Conclusions: These results demonstrate that microRNA let-7c is downregulated in PCa and functions as a tumor suppressor, and is a potential therapeutic target for PCa. © 2012 Nadiminty et al.
AB - Purpose: Prostate cancer (PCa) is characterized by deregulated expression of several tumor suppressor or oncogenic miRNAs. The objective of this study was the identification and characterization of miR-let-7c as a potential tumor suppressor in PCa. Experimental Design: Levels of expression of miR-let-7c were examined in human PCa cell lines and tissues using qRT-PCR and in situ hybridization. Let-7c was overexpressed or suppressed to assess the effects on the growth of human PCa cell lines. Lentiviral-mediated re-expression of let-7c was utilized to assess the effects on human PCa xenografts. Results: We identified miR-let-7c as a potential tumor suppressor in PCa. Expression of let-7c is downregulated in castration-resistant prostate cancer (CRPC) cells. Overexpression of let-7c decreased while downregulation of let-7c increased cell proliferation, clonogenicity and anchorage-independent growth of PCa cells in vitro. Suppression of let-7c expression enhanced the ability of androgen-sensitive PCa cells to grow in androgen-deprived conditions in vitro. Reconstitution of Let-7c by lentiviral-mediated intratumoral delivery significantly reduced tumor burden in xenografts of human PCa cells. Furthermore, let-7c expression is downregulated in clinical PCa specimens compared to their matched benign tissues, while the expression of Lin28, a master regulator of let-7 miRNA processing, is upregulated in clinical PCa specimens. Conclusions: These results demonstrate that microRNA let-7c is downregulated in PCa and functions as a tumor suppressor, and is a potential therapeutic target for PCa. © 2012 Nadiminty et al.
KW - androgen
KW - lentivirus vector
KW - microRNA
KW - microRNA let 7c
KW - regulator protein
KW - tumor suppressor protein
KW - unclassified drug
KW - green fluorescent protein
KW - mirnlet7 microRNA, human
KW - animal cell
KW - animal experiment
KW - animal tissue
KW - article
KW - cancer cell culture
KW - cancer growth
KW - cancer inhibition
KW - castration resistant prostate cancer
KW - cell growth
KW - cell proliferation
KW - clonogenesis
KW - controlled study
KW - down regulation
KW - drug targeting
KW - gene overexpression
KW - human
KW - human cell
KW - in situ hybridization
KW - in vitro study
KW - male
KW - mouse
KW - nonhuman
KW - protein expression
KW - protein function
KW - reverse transcription polymerase chain reaction
KW - RNA processing
KW - tumor volume
KW - tumor xenograft
KW - upregulation
KW - animal
KW - experimental neoplasm
KW - gene expression regulation
KW - genetics
KW - Lentivirinae
KW - metabolism
KW - Northern blotting
KW - nude mouse
KW - orchiectomy
KW - pathology
KW - prostate tumor
KW - tumor cell line
KW - tumor suppressor gene
KW - xenograft
KW - Androgens
KW - Animals
KW - Blotting, Northern
KW - Cell Line, Tumor
KW - Cell Proliferation
KW - Down-Regulation
KW - Gene Expression Regulation, Neoplastic
KW - Genes, Tumor Suppressor
KW - Green Fluorescent Proteins
KW - Humans
KW - In Situ Hybridization
KW - Lentivirus
KW - Male
KW - Mice
KW - Mice, Nude
KW - MicroRNAs
KW - Neoplasms, Experimental
KW - Orchiectomy
KW - Prostatic Neoplasms
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Transplantation, Heterologous
KW - Tumor Burden
U2 - 10.1371/journal.pone.0032832
DO - 10.1371/journal.pone.0032832
M3 - Article
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 3
ER -