@article{e9dd494c5867453692bb573464def2e9,
title = "MicroRNA let-7c is downregulated in prostate cancer and suppresses prostate cancer growth",
abstract = "Purpose: Prostate cancer (PCa) is characterized by deregulated expression of several tumor suppressor or oncogenic miRNAs. The objective of this study was the identification and characterization of miR-let-7c as a potential tumor suppressor in PCa. Experimental Design: Levels of expression of miR-let-7c were examined in human PCa cell lines and tissues using qRT-PCR and in situ hybridization. Let-7c was overexpressed or suppressed to assess the effects on the growth of human PCa cell lines. Lentiviral-mediated re-expression of let-7c was utilized to assess the effects on human PCa xenografts. Results: We identified miR-let-7c as a potential tumor suppressor in PCa. Expression of let-7c is downregulated in castration-resistant prostate cancer (CRPC) cells. Overexpression of let-7c decreased while downregulation of let-7c increased cell proliferation, clonogenicity and anchorage-independent growth of PCa cells in vitro. Suppression of let-7c expression enhanced the ability of androgen-sensitive PCa cells to grow in androgen-deprived conditions in vitro. Reconstitution of Let-7c by lentiviral-mediated intratumoral delivery significantly reduced tumor burden in xenografts of human PCa cells. Furthermore, let-7c expression is downregulated in clinical PCa specimens compared to their matched benign tissues, while the expression of Lin28, a master regulator of let-7 miRNA processing, is upregulated in clinical PCa specimens. Conclusions: These results demonstrate that microRNA let-7c is downregulated in PCa and functions as a tumor suppressor, and is a potential therapeutic target for PCa. {\textcopyright} 2012 Nadiminty et al.",
keywords = "androgen, lentivirus vector, microRNA, microRNA let 7c, regulator protein, tumor suppressor protein, unclassified drug, green fluorescent protein, mirnlet7 microRNA, human, animal cell, animal experiment, animal tissue, article, cancer cell culture, cancer growth, cancer inhibition, castration resistant prostate cancer, cell growth, cell proliferation, clonogenesis, controlled study, down regulation, drug targeting, gene overexpression, human, human cell, in situ hybridization, in vitro study, male, mouse, nonhuman, protein expression, protein function, reverse transcription polymerase chain reaction, RNA processing, tumor volume, tumor xenograft, upregulation, animal, experimental neoplasm, gene expression regulation, genetics, Lentivirinae, metabolism, Northern blotting, nude mouse, orchiectomy, pathology, prostate tumor, tumor cell line, tumor suppressor gene, xenograft, Androgens, Animals, Blotting, Northern, Cell Line, Tumor, Cell Proliferation, Down-Regulation, Gene Expression Regulation, Neoplastic, Genes, Tumor Suppressor, Green Fluorescent Proteins, Humans, In Situ Hybridization, Lentivirus, Male, Mice, Mice, Nude, MicroRNAs, Neoplasms, Experimental, Orchiectomy, Prostatic Neoplasms, Reverse Transcriptase Polymerase Chain Reaction, Transplantation, Heterologous, Tumor Burden",
author = "N. Nadiminty and R. Tummala and W. Lou and Y. Zhu and X.-B. Shi and J.X. Zou and H. Chen and J. Zhang and X. Chen and J. Luo and {deVere White}, R.W. and H.-J. Kung and C.P. Evans and A.C. Gao",
note = "引用次數:82 Export Date: 5 March 2018 通訊地址: Nadiminty, N.; Department of Urology, University of California Davis, Sacramento, CA, United States; 電子郵件: acgao@ucdavis.edu 化學物質/CAS: Androgens; Green Fluorescent Proteins, 147336-22-9; MicroRNAs; mirnlet7 microRNA, human 參考文獻: Shi, X.-B., Tepper, C.G., deVere White, R.W., Cancerous miRNAs and their regulation Cell Cycle (2008), 7, pp. 1529-1538; Coppola, V., de Maria, R., Bonci, D., (2009) MicroRNAs and prostate cancer, pp. ER0170-ER0172. , Endocr Relat Cancer; Gandellini, P., Folini, M., Zaffaroni, N., Towards the definition of prostate cancer-related microRNAs: where are we now? 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year = "2012",
month = mar,
doi = "10.1371/journal.pone.0032832",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",
}
