MicroRNA 363 mediated positive regulation of c-myc translation affect prostate cancer development and progress

Y. Chen, X. Lu, B. Wu, Y. Su, J. Li, H. Wang

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Prostate cancer (CaP) is the sixth most significant cancer killer of men in China. In this study, the potential role of micro-363 (miR-363) in CaP development and progression was investigated. Pri-miR-363 or anti-miR-363 was transfected into the CaP cells line PC-3 cells. Cell proliferation, transformation property, and epithelial-to-mesenchymal transition (EMT) were evaluated by MTT, clonogenic assay, colony formation in soft agar and western blotting, respectively. The expression and involvement of c-myc, a downstream target of miR-363 were also determined. The results showed that endogenous expression of miR-363 was significantly increased in CaP cells compared with normal prostate cells. High expression of miR-363 in PC-3 cells through transfection induces cell proliferation and positively regulates cell transformation property as well as promotes EMT of PC-3 cells. Through knockdown of c-myc, the results also showed that c-myc was involved in the regulation of biological function of PC-3 cells by miR-363. Taken together, this study adds support to the potential role of miR-363 in the diagnosis and treatment of CaP.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalNeoplasma
Volume62
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • Epithelial-to-mesenchymal transition
  • Micro-363
  • Proliferation
  • Prostate cancer
  • Transformation property

ASJC Scopus subject areas

  • Cancer Research

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