MicroRNA-224 suppresses colorectal cancer cell migration by targeting Cdc42

Tao Wei Ke, Han Lin Hsu, Yu Hua Wu, William Tzu Liang Chen, Ya Wen Cheng, Chao Wen Cheng

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The metastatic spread of tumor cells is the major risk factor affecting the clinical prognosis of colorectal cancer (CRC) patients. The metastatic phenotype can be modulated by dysregulating the synthesis of different structural and functional proteins of tumor cells. Micro(mi)RNAs are noncoding RNAs that recognize their cognate messenger (m)RNA targets by sequence-specific interactions with the 3′ untranslated region and are involved in the multistep process of CRC development. The objective of this study was to investigate the expression and biological roles of miR-224 in CRC. The miR-224 expression level was assessed by a quantitative real-time PCR in 79 CRC and 18 nontumor tissues. Expression levels of miR-224 in CRC tissues were significantly lower than those in nontumor tissues. Its expression level was associated with the mutation status of the APC gene. Ectopic expression of miR-224 suppressed the migratory ability of CRC cell line, but cell proliferation was less affected. Increased miR-224 diminished Cdc42 and SMAD4 expressions at both the protein and mRNA levels and inhibited the formation of actin filaments. Overall, this study indicated a role of miR-224 in negatively regulating CRC cell migration. The expression level of miR-224 may be a useful predictive biomarker for CRC progression.

Original languageEnglish
Article number617150
JournalDisease Markers
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

MicroRNAs
Cell Movement
Colorectal Neoplasms
Cells
Tissue
Tumors
Untranslated RNA
Cell proliferation
3' Untranslated Regions
Biomarkers
Actins
Proteins
Genes
RNA
Messenger RNA
APC Genes
Actin Cytoskeleton
Real-Time Polymerase Chain Reaction
Neoplasms
Cell Proliferation

ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

MicroRNA-224 suppresses colorectal cancer cell migration by targeting Cdc42. / Ke, Tao Wei; Hsu, Han Lin; Wu, Yu Hua; Chen, William Tzu Liang; Cheng, Ya Wen; Cheng, Chao Wen.

In: Disease Markers, Vol. 2014, 617150, 2014.

Research output: Contribution to journalArticle

Ke, Tao Wei ; Hsu, Han Lin ; Wu, Yu Hua ; Chen, William Tzu Liang ; Cheng, Ya Wen ; Cheng, Chao Wen. / MicroRNA-224 suppresses colorectal cancer cell migration by targeting Cdc42. In: Disease Markers. 2014 ; Vol. 2014.
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