MicroRNA-19a and microRNA-19b promote the malignancy of clear cell renal cell carcinoma through targeting the tumor suppressor RhoB

Shaoxi Niu, Xin Ma, Yu Zhang, Yen Nien Liu, Xufeng Chen, Huijie Gong, Yuanxin Yao, Kan Liu, Xu Zhang

Research output: Contribution to journalArticle

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Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal cell carcinoma, which shows high aggressiveness and lacks biomarkers. RhoB acts as a tumor suppressor that inhibits the progression of ccRCC. In the present study, we examined the effects of oncogenic microRNAs, miR-19a and miR-19b, on RhoB expression in ccRCC cells. The results showed that both miR-19a and miR-19b could directly target the 30untranslated region (3’UTR) of RhoB, resulting in the reduced expression of RhoB. With RT-PCR analysis, we detected the increased expression of miR-19a and miR-19b in ccRCC tissues compared to adjacent non-tumor renal tissues. These data also demonstrated an exclusive negative correlation between miR-19a/19b and RhoB expression in ccRCC specimens and cell lines. In addition, the knockdown of RhoB or overexpression of miR-19a and miR-19b in ccRCC cells could promote cell proliferation, migration and invasion. These data demonstrate the direct roles of miR-19a and miR-19b on the repression of RhoB and its consequences on tumorigenesis, cancer cell proliferation and invasiveness. These results suggest the potential clinical impact of miR-19a and miR-19b as molecular targets for ccRCC.

Original languageEnglish
Article numbere0192790
JournalPLoS One
Volume13
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

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kidney cells
MicroRNAs
microRNA
Renal Cell Carcinoma
carcinoma
Tumors
Cells
Cell proliferation
neoplasms
Tissue
Neoplasms
Biomarkers
cells
cell proliferation
Cell Proliferation
Cell Movement
carcinogenesis
Carcinogenesis
aggression
biomarkers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

MicroRNA-19a and microRNA-19b promote the malignancy of clear cell renal cell carcinoma through targeting the tumor suppressor RhoB. / Niu, Shaoxi; Ma, Xin; Zhang, Yu; Liu, Yen Nien; Chen, Xufeng; Gong, Huijie; Yao, Yuanxin; Liu, Kan; Zhang, Xu.

In: PLoS One, Vol. 13, No. 2, e0192790, 01.02.2018.

Research output: Contribution to journalArticle

Niu, Shaoxi ; Ma, Xin ; Zhang, Yu ; Liu, Yen Nien ; Chen, Xufeng ; Gong, Huijie ; Yao, Yuanxin ; Liu, Kan ; Zhang, Xu. / MicroRNA-19a and microRNA-19b promote the malignancy of clear cell renal cell carcinoma through targeting the tumor suppressor RhoB. In: PLoS One. 2018 ; Vol. 13, No. 2.
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