Abstract

Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for crosslinking in fabrication of the desired gelatin-graphene oxide (gel-GO) nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8% w/v) containing 0.1~1 wt% of high-functionality nano-graphene oxide (GO). Optimized plasma conditions (2,500 V and 8.7 mA) for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 mm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50% with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1% (w/w) of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasmaassisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications.

Original languageEnglish
Pages (from-to)e3498
JournalPeerJ
Volume2017
Issue number6
DOIs
Publication statusPublished - 2017

Fingerprint

nanocomposites
Graphite
Hydrogel
hydrocolloids
Gelatin
gelatin
Oxides
Fabrication
synthesis
crosslinking
Assays
Composite materials
Nanocomposites
Hydrogels
Argon
tissue engineering
Tissue Engineering
contact angle
Tissue engineering
assays

Keywords

  • Argon microplasma
  • Biocompatibility
  • Cross-linking
  • Gelatin
  • Graphene oxide
  • Hydrogel
  • Tissue engineering

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Microplasma-assisted hydrogel fabrication : A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application. / Satapathy, Mantosh Kumar; Chiang, Wei Hung; Chuang, Er Yuan; Chen, Chih Hwa; Liao, Jia Liang; Huang, Huin Ning.

In: PeerJ, Vol. 2017, No. 6, 2017, p. e3498.

Research output: Contribution to journalArticle

Satapathy, Mantosh Kumar ; Chiang, Wei Hung ; Chuang, Er Yuan ; Chen, Chih Hwa ; Liao, Jia Liang ; Huang, Huin Ning. / Microplasma-assisted hydrogel fabrication : A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application. In: PeerJ. 2017 ; Vol. 2017, No. 6. pp. e3498.
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abstract = "Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for crosslinking in fabrication of the desired gelatin-graphene oxide (gel-GO) nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8{\%} w/v) containing 0.1~1 wt{\%} of high-functionality nano-graphene oxide (GO). Optimized plasma conditions (2,500 V and 8.7 mA) for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 mm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50{\%} with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1{\%} (w/w) of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasmaassisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications.",
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