Microglia/macrophages responses to kainate-induced injury in the rat retina

Min L. Chang, Ching Hsiang Wu, Hsiung F. Chien, Ya F. Jiang-Shieh, Jeng Yung Shieh, Chen Yuan Wen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The present study was aimed to elucidate how retinal microglia/macrophages would respond to neuronal death after intravitreal kainate injection. An increased expression of the complement receptor type 3 (CR3) and an induction of the major histocompatibility complex (MHC) class II and ED-1 antigens were mainly observed in the inner retina after kainate injection. Prominent cell death revealed by Fluoro Jade B (FJB) staining and ultrastructural examination appeared at the inner border of the inner nuclear layer (INL) at 1 day post-injection. Interestingly, some immunoreactive cells appeared at the outer segment of photoreceptor layer (OSPRL) at different time intervals. Our quantitative analysis further showed that CR3 immunoreactivity was drastically increased peaking at 7 days but subsided thereafter. MHC class II and ED-1 immunoreactivities showed a moderate but steady increase peaking at 3 days and declined thereafter. Double labeling study further revealed that retinal microglia/macrophages expressed concurrently CR3 and ED-1 antigens (OX-42 +/ED-1+) or MHC class II molecules (OX-42 +/OX-6+) and remained branched in shape at early stage of kainate challenge. By electron microscopy, microglia/macrophages with CR3 immunoreactivity displayed abundant cytoplasm containing a few vesicles and phagosomes. Other cells ultrastructurally similar to Müller cells or astrocytes could also engulf exogenous substances. In conclusion, retinal microglia/macrophages responded vigorously to kainate-induced neuronal cell death that may also trigger the recruitment of macrophages from neighboring tissues and induce the phagocytotic activity of cells other than retinal microglia/macrophages.

Original languageEnglish
Pages (from-to)202-212
Number of pages11
JournalNeuroscience Research
Volume54
Issue number3
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

Fingerprint

Kainic Acid
Microglia
Macrophage-1 Antigen
Retina
Macrophages
Wounds and Injuries
Major Histocompatibility Complex
Cell Death
Antigens
Phagosomes
Intravitreal Injections
Injections
Astrocytes
Electron Microscopy
Cytoplasm
Staining and Labeling

Keywords

  • Intravitreal injection
  • Kainate
  • Microglia/macrophages
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Microglia/macrophages responses to kainate-induced injury in the rat retina. / Chang, Min L.; Wu, Ching Hsiang; Chien, Hsiung F.; Jiang-Shieh, Ya F.; Shieh, Jeng Yung; Wen, Chen Yuan.

In: Neuroscience Research, Vol. 54, No. 3, 03.2006, p. 202-212.

Research output: Contribution to journalArticle

Chang, Min L. ; Wu, Ching Hsiang ; Chien, Hsiung F. ; Jiang-Shieh, Ya F. ; Shieh, Jeng Yung ; Wen, Chen Yuan. / Microglia/macrophages responses to kainate-induced injury in the rat retina. In: Neuroscience Research. 2006 ; Vol. 54, No. 3. pp. 202-212.
@article{73438dc067724d8086ac2f1dc78b924e,
title = "Microglia/macrophages responses to kainate-induced injury in the rat retina",
abstract = "The present study was aimed to elucidate how retinal microglia/macrophages would respond to neuronal death after intravitreal kainate injection. An increased expression of the complement receptor type 3 (CR3) and an induction of the major histocompatibility complex (MHC) class II and ED-1 antigens were mainly observed in the inner retina after kainate injection. Prominent cell death revealed by Fluoro Jade B (FJB) staining and ultrastructural examination appeared at the inner border of the inner nuclear layer (INL) at 1 day post-injection. Interestingly, some immunoreactive cells appeared at the outer segment of photoreceptor layer (OSPRL) at different time intervals. Our quantitative analysis further showed that CR3 immunoreactivity was drastically increased peaking at 7 days but subsided thereafter. MHC class II and ED-1 immunoreactivities showed a moderate but steady increase peaking at 3 days and declined thereafter. Double labeling study further revealed that retinal microglia/macrophages expressed concurrently CR3 and ED-1 antigens (OX-42 +/ED-1+) or MHC class II molecules (OX-42 +/OX-6+) and remained branched in shape at early stage of kainate challenge. By electron microscopy, microglia/macrophages with CR3 immunoreactivity displayed abundant cytoplasm containing a few vesicles and phagosomes. Other cells ultrastructurally similar to M{\"u}ller cells or astrocytes could also engulf exogenous substances. In conclusion, retinal microglia/macrophages responded vigorously to kainate-induced neuronal cell death that may also trigger the recruitment of macrophages from neighboring tissues and induce the phagocytotic activity of cells other than retinal microglia/macrophages.",
keywords = "Intravitreal injection, Kainate, Microglia/macrophages, Retina",
author = "Chang, {Min L.} and Wu, {Ching Hsiang} and Chien, {Hsiung F.} and Jiang-Shieh, {Ya F.} and Shieh, {Jeng Yung} and Wen, {Chen Yuan}",
year = "2006",
month = "3",
doi = "10.1016/j.neures.2005.11.010",
language = "English",
volume = "54",
pages = "202--212",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Microglia/macrophages responses to kainate-induced injury in the rat retina

AU - Chang, Min L.

AU - Wu, Ching Hsiang

AU - Chien, Hsiung F.

AU - Jiang-Shieh, Ya F.

AU - Shieh, Jeng Yung

AU - Wen, Chen Yuan

PY - 2006/3

Y1 - 2006/3

N2 - The present study was aimed to elucidate how retinal microglia/macrophages would respond to neuronal death after intravitreal kainate injection. An increased expression of the complement receptor type 3 (CR3) and an induction of the major histocompatibility complex (MHC) class II and ED-1 antigens were mainly observed in the inner retina after kainate injection. Prominent cell death revealed by Fluoro Jade B (FJB) staining and ultrastructural examination appeared at the inner border of the inner nuclear layer (INL) at 1 day post-injection. Interestingly, some immunoreactive cells appeared at the outer segment of photoreceptor layer (OSPRL) at different time intervals. Our quantitative analysis further showed that CR3 immunoreactivity was drastically increased peaking at 7 days but subsided thereafter. MHC class II and ED-1 immunoreactivities showed a moderate but steady increase peaking at 3 days and declined thereafter. Double labeling study further revealed that retinal microglia/macrophages expressed concurrently CR3 and ED-1 antigens (OX-42 +/ED-1+) or MHC class II molecules (OX-42 +/OX-6+) and remained branched in shape at early stage of kainate challenge. By electron microscopy, microglia/macrophages with CR3 immunoreactivity displayed abundant cytoplasm containing a few vesicles and phagosomes. Other cells ultrastructurally similar to Müller cells or astrocytes could also engulf exogenous substances. In conclusion, retinal microglia/macrophages responded vigorously to kainate-induced neuronal cell death that may also trigger the recruitment of macrophages from neighboring tissues and induce the phagocytotic activity of cells other than retinal microglia/macrophages.

AB - The present study was aimed to elucidate how retinal microglia/macrophages would respond to neuronal death after intravitreal kainate injection. An increased expression of the complement receptor type 3 (CR3) and an induction of the major histocompatibility complex (MHC) class II and ED-1 antigens were mainly observed in the inner retina after kainate injection. Prominent cell death revealed by Fluoro Jade B (FJB) staining and ultrastructural examination appeared at the inner border of the inner nuclear layer (INL) at 1 day post-injection. Interestingly, some immunoreactive cells appeared at the outer segment of photoreceptor layer (OSPRL) at different time intervals. Our quantitative analysis further showed that CR3 immunoreactivity was drastically increased peaking at 7 days but subsided thereafter. MHC class II and ED-1 immunoreactivities showed a moderate but steady increase peaking at 3 days and declined thereafter. Double labeling study further revealed that retinal microglia/macrophages expressed concurrently CR3 and ED-1 antigens (OX-42 +/ED-1+) or MHC class II molecules (OX-42 +/OX-6+) and remained branched in shape at early stage of kainate challenge. By electron microscopy, microglia/macrophages with CR3 immunoreactivity displayed abundant cytoplasm containing a few vesicles and phagosomes. Other cells ultrastructurally similar to Müller cells or astrocytes could also engulf exogenous substances. In conclusion, retinal microglia/macrophages responded vigorously to kainate-induced neuronal cell death that may also trigger the recruitment of macrophages from neighboring tissues and induce the phagocytotic activity of cells other than retinal microglia/macrophages.

KW - Intravitreal injection

KW - Kainate

KW - Microglia/macrophages

KW - Retina

UR - http://www.scopus.com/inward/record.url?scp=33244461510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33244461510&partnerID=8YFLogxK

U2 - 10.1016/j.neures.2005.11.010

DO - 10.1016/j.neures.2005.11.010

M3 - Article

C2 - 16458383

AN - SCOPUS:33244461510

VL - 54

SP - 202

EP - 212

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

IS - 3

ER -