Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage

Gilbert A. Lee, Chi Hsin Lin, Hang Hsiang Jiang, Hsin Ju Chao, Chieh Liang Wu, Chi Mei Hsueh

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalNeuroscience Letters
Volume356
Issue number2
DOIs
Publication statusPublished - Feb 12 2004

Keywords

  • Astrocyte
  • Glial cell line-derived neurotrophic factor
  • In vitro ischemia
  • Microglia

ASJC Scopus subject areas

  • Neuroscience(all)

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