Microfluidic channel integrated with a lattice lightsheet microscopic system for continuous cell imaging

Yu Jui Fan, Han Yun Hsieh, Sheng Fang Tsai, Cheng Hsuan Wu, Chia Ming Lee, Yen Ting Liu, Chieh Han Lu, Shu Wei Chang, Bi Chang Chen

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In this study, a continuous cell-imaging system with subcellular resolution was developed by integrating a microfluidic platform with lattice lightsheet microscopy (LLSM). To reduce aberrations of the lightsheet propagating into the device, a microfluidic channel sealed with a water refractive index-matched thin film was fabricated. When the lightsheet emerged from the water-immersed objectives and penetrated through the water refractive-matched thin film into the microfluidic channel at an incident angle, less light scattering and fewer aberrations were found. Suspended cells flowed across the lattice lightsheet, and an imaging system with the image plane perpendicular to the lightsheet was used to sequentially acquire cell images. By applying a thinner lattice lightsheet, higher-resolution, higher-contrast images were obtained. Furthermore, three-dimensional cell images could be achieved by reconstructing sequential two-dimensional cell images. This journal is

Original languageEnglish
Pages (from-to)344-354
Number of pages11
JournalLab on a Chip
Volume21
Issue number2
DOIs
Publication statusPublished - Jan 21 2021

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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