Microdialysis for measurement of hepatic and systemic nitric oxide biosynthesis in septic rats

C. J. Huang, P. S. Tsai, Wynn H T Pan, J. W. Skimmings

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: We sought to compare two techniques, microdialysis and repeated blood withdrawal, for serial assessment of hepatic and systemic nitric oxide (NO) biosynthesis in septic rats. Methods: Rats were randomly allocated to either microdialysis or blood withdrawal groups. Two microdialysis probes, one in liver and the other in right atrium, were placed in rats in the microdialysis group. Half of the rats from each group were then given lipopolysaccharide (LPS) to induce NO production. The other half of the rats from each group were injected with vehicle (normal saline) to serve as controls. In the microdialysis group, dialysate (30 μl) was collected every 30 min. In the blood withdrawal group, 0.3 ml of blood was drawn every 30 min. Sampling was performed up to 6 h after injection of LPS or vehicle. Hemodynamics, hepatic and systemic NO concentrations, and iNOS expression in harvested liver tissues were assayed. Results: Repeated blood withdrawal by itself caused a significant decrease in blood pressure and induced hepatic iNOS expression. Microdialysis, on the contrary, reliably detected LPS-induced NO production without resulting either in hemodynamic changes or in iNOS induction in liver tissue. Conclusions: Microdialysis provides serial measure of hepatic and systemic NO concentrations in LPS-treated rats without the need for removal of tissue.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalActa Anaesthesiologica Scandinavica
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Microdialysis
Nitric Oxide
Liver
Lipopolysaccharides
Blood Group Antigens
Hemodynamics
Dialysis Solutions
Heart Atria
Blood Pressure
Injections

Keywords

  • INOS
  • Liver
  • Microdialysis
  • Nitric oxide
  • Rat
  • Sepsis

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Microdialysis for measurement of hepatic and systemic nitric oxide biosynthesis in septic rats. / Huang, C. J.; Tsai, P. S.; Pan, Wynn H T; Skimmings, J. W.

In: Acta Anaesthesiologica Scandinavica, Vol. 49, No. 1, 01.2005, p. 28-34.

Research output: Contribution to journalArticle

@article{6f9502b334f64e20b5df2f5351bbe9e3,
title = "Microdialysis for measurement of hepatic and systemic nitric oxide biosynthesis in septic rats",
abstract = "Background: We sought to compare two techniques, microdialysis and repeated blood withdrawal, for serial assessment of hepatic and systemic nitric oxide (NO) biosynthesis in septic rats. Methods: Rats were randomly allocated to either microdialysis or blood withdrawal groups. Two microdialysis probes, one in liver and the other in right atrium, were placed in rats in the microdialysis group. Half of the rats from each group were then given lipopolysaccharide (LPS) to induce NO production. The other half of the rats from each group were injected with vehicle (normal saline) to serve as controls. In the microdialysis group, dialysate (30 μl) was collected every 30 min. In the blood withdrawal group, 0.3 ml of blood was drawn every 30 min. Sampling was performed up to 6 h after injection of LPS or vehicle. Hemodynamics, hepatic and systemic NO concentrations, and iNOS expression in harvested liver tissues were assayed. Results: Repeated blood withdrawal by itself caused a significant decrease in blood pressure and induced hepatic iNOS expression. Microdialysis, on the contrary, reliably detected LPS-induced NO production without resulting either in hemodynamic changes or in iNOS induction in liver tissue. Conclusions: Microdialysis provides serial measure of hepatic and systemic NO concentrations in LPS-treated rats without the need for removal of tissue.",
keywords = "INOS, Liver, Microdialysis, Nitric oxide, Rat, Sepsis",
author = "Huang, {C. J.} and Tsai, {P. S.} and Pan, {Wynn H T} and Skimmings, {J. W.}",
year = "2005",
month = "1",
doi = "10.1111/j.1399-6576.2005.00486.x",
language = "English",
volume = "49",
pages = "28--34",
journal = "Acta Anaesthesiologica Scandinavica",
issn = "0001-5172",
publisher = "Blackwell Munksgaard",
number = "1",

}

TY - JOUR

T1 - Microdialysis for measurement of hepatic and systemic nitric oxide biosynthesis in septic rats

AU - Huang, C. J.

AU - Tsai, P. S.

AU - Pan, Wynn H T

AU - Skimmings, J. W.

PY - 2005/1

Y1 - 2005/1

N2 - Background: We sought to compare two techniques, microdialysis and repeated blood withdrawal, for serial assessment of hepatic and systemic nitric oxide (NO) biosynthesis in septic rats. Methods: Rats were randomly allocated to either microdialysis or blood withdrawal groups. Two microdialysis probes, one in liver and the other in right atrium, were placed in rats in the microdialysis group. Half of the rats from each group were then given lipopolysaccharide (LPS) to induce NO production. The other half of the rats from each group were injected with vehicle (normal saline) to serve as controls. In the microdialysis group, dialysate (30 μl) was collected every 30 min. In the blood withdrawal group, 0.3 ml of blood was drawn every 30 min. Sampling was performed up to 6 h after injection of LPS or vehicle. Hemodynamics, hepatic and systemic NO concentrations, and iNOS expression in harvested liver tissues were assayed. Results: Repeated blood withdrawal by itself caused a significant decrease in blood pressure and induced hepatic iNOS expression. Microdialysis, on the contrary, reliably detected LPS-induced NO production without resulting either in hemodynamic changes or in iNOS induction in liver tissue. Conclusions: Microdialysis provides serial measure of hepatic and systemic NO concentrations in LPS-treated rats without the need for removal of tissue.

AB - Background: We sought to compare two techniques, microdialysis and repeated blood withdrawal, for serial assessment of hepatic and systemic nitric oxide (NO) biosynthesis in septic rats. Methods: Rats were randomly allocated to either microdialysis or blood withdrawal groups. Two microdialysis probes, one in liver and the other in right atrium, were placed in rats in the microdialysis group. Half of the rats from each group were then given lipopolysaccharide (LPS) to induce NO production. The other half of the rats from each group were injected with vehicle (normal saline) to serve as controls. In the microdialysis group, dialysate (30 μl) was collected every 30 min. In the blood withdrawal group, 0.3 ml of blood was drawn every 30 min. Sampling was performed up to 6 h after injection of LPS or vehicle. Hemodynamics, hepatic and systemic NO concentrations, and iNOS expression in harvested liver tissues were assayed. Results: Repeated blood withdrawal by itself caused a significant decrease in blood pressure and induced hepatic iNOS expression. Microdialysis, on the contrary, reliably detected LPS-induced NO production without resulting either in hemodynamic changes or in iNOS induction in liver tissue. Conclusions: Microdialysis provides serial measure of hepatic and systemic NO concentrations in LPS-treated rats without the need for removal of tissue.

KW - INOS

KW - Liver

KW - Microdialysis

KW - Nitric oxide

KW - Rat

KW - Sepsis

UR - http://www.scopus.com/inward/record.url?scp=13444305380&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13444305380&partnerID=8YFLogxK

U2 - 10.1111/j.1399-6576.2005.00486.x

DO - 10.1111/j.1399-6576.2005.00486.x

M3 - Article

C2 - 15675978

AN - SCOPUS:13444305380

VL - 49

SP - 28

EP - 34

JO - Acta Anaesthesiologica Scandinavica

JF - Acta Anaesthesiologica Scandinavica

SN - 0001-5172

IS - 1

ER -