Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods

Shiou Huei Chao, Hui Yu Huang, Chuan Hsiung Chang, Chih Hsien Yang, Wei Shen Cheng, Ya Huei Kang, Koichi Watanabe, Ying Chieh Tsai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In Taiwanese alternative medicine Lu-doh-huang (also called Pracparatum mungo), mung beans are mixed with various herbal medicines and undergo a 4-stage process of anaerobic fermentation. Here we used high-throughput sequencing of the 16S rRNA gene to profile the bacterial community structure of Lu-doh-huang samples. Pyrosequencing of samples obtained at 7 points during fermentation revealed 9 phyla, 264 genera, and 586 species of bacteria. While mung beans were inside bamboo sections (stages 1 and 2 of the fermentation process), family Lactobacillaceae and genus Lactobacillus emerged in highest abundance; Lactobacillus plantarum was broadly distributed among these samples. During stage 3, the bacterial distribution shifted to family Porphyromonadaceae, and Butyricimonas virosa became the predominant microbial component. Thereafter, bacterial counts decreased dramatically, and organisms were too few to be detected during stage 4. In addition, the microbial compositions of the liquids used for soaking bamboo sections were dramatically different: Exiguobacterium mexicanum predominated in the fermented soybean solution whereas B. virosa was predominant in running spring water. Furthermore, our results from pyrosequencing paralleled those we obtained by using the traditional culture method, which targets lactic acid bacteria. In conclusion, the microbial communities during Lu-doh-huang fermentation were markedly diverse, and pyrosequencing revealed a complete picture of the microbial consortium.

Original languageEnglish
Article numbere63816
JournalPLoS ONE
Volume8
Issue number5
DOIs
Publication statusPublished - May 20 2013
Externally publishedYes

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mung beans
Fermentation
fermentation
Bamboo
bamboos
Lactobacillaceae
Bacteria
Exiguobacterium
Microbial Consortia
Bacterial Structures
Lactobacillus plantarum
alternative medicine
Springs (water)
Bacterial Load
Herbal Medicine
herbal medicines
Lactobacillus
Complementary Therapies
methodology
rRNA Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods. / Chao, Shiou Huei; Huang, Hui Yu; Chang, Chuan Hsiung; Yang, Chih Hsien; Cheng, Wei Shen; Kang, Ya Huei; Watanabe, Koichi; Tsai, Ying Chieh.

In: PLoS ONE, Vol. 8, No. 5, e63816, 20.05.2013.

Research output: Contribution to journalArticle

Chao, Shiou Huei ; Huang, Hui Yu ; Chang, Chuan Hsiung ; Yang, Chih Hsien ; Cheng, Wei Shen ; Kang, Ya Huei ; Watanabe, Koichi ; Tsai, Ying Chieh. / Microbial Diversity Analysis of Fermented Mung Beans (Lu-Doh-Huang) by Using Pyrosequencing and Culture Methods. In: PLoS ONE. 2013 ; Vol. 8, No. 5.
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