Methylomic analysis identifies frequent DNA methylation of Zinc Finger protein 582 (ZNF582) in cervical neoplasms

Rui Lan Huang, Cheng Chang Chang, Po Hsuan Su, Yu Chih Chen, Yu Ping Liao, Hui Chen Wang, Yi Te Yo, Tai Kuang Chao, Hsuan Cheng Huang, Ching Yu Lin, Tang Yuan Chu, Hung Cheng Lai

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53 Citations (Scopus)

Abstract

Background: Despite of the trend that the application of DNA methylation as a biomarker for cancer detection is promising, clinically applicable genes are few. Therefore, we looked for novel hypermethylated genes for cervical cancer screening. Methods and Findings: At the discovery phase, we analyzed the methylation profiles of human cervical carcinomas and normal cervixes by methylated DNA immunoprecipitation coupled to promoter tiling arrays (MeDIP-on-chip). Methylation-specific PCR (MSP), quantitative MSP and bisulfite sequencing were used to verify the methylation status in cancer tissues and cervical scrapings from patients with different severities. Immunohistochemical staining of a cervical tissue microarray was used to confirm protein expression. We narrowed to three candidate genes: DBC1, PDE8B, and ZNF582; their methylation frequencies in tumors were 93%, 29%, and 100%, respectively. At the pre-validation phase, the methylation frequency of DBC1 and ZNF582 in cervical scraping correlated significantly with disease severity in an independent cohort (n = 330, both P

Original languageEnglish
Article numbere41060
JournalPLoS One
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 16 2012

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Huang, R. L., Chang, C. C., Su, P. H., Chen, Y. C., Liao, Y. P., Wang, H. C., Yo, Y. T., Chao, T. K., Huang, H. C., Lin, C. Y., Chu, T. Y., & Lai, H. C. (2012). Methylomic analysis identifies frequent DNA methylation of Zinc Finger protein 582 (ZNF582) in cervical neoplasms. PLoS One, 7(7), [e41060]. https://doi.org/10.1371/journal.pone.0041060