Methoxychalcone induces cell-cycle arrest and apoptosis in human hormone-resistant prostate cancer cells through PI 3-kinase-independent inhibition of mTOR pathways

Yu Wei Sun, Wei Jan Huang, Che Jen Hsiao, Yi Cheng Chen, Pin Hsuan Lu, Jih Hwa Guh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

BACKGROUND. Chalcones are contained in fruits and vegetables, and have been suggested to display anticancer activities. In this study, the anticancer mechanism of WJ9708011 (a methoxychalcone derivative) was delineated in human prostate cancer cells. METHOD. Cell proliferation was examined by sulforhodamine B and clonogenic assays. Cell-cycle progression and mitochondrial membrane potential (Δψm) were detected by flow cytometric analysis. Expressions of protein and mRNA were detected by Western blot and RT-PCR technique, respectively. The protein synthesis was examined by [ 3H]leucine incorporation assay. The overexpression or knockdown techniques for specific target protein were also used in this study. RESULTS. WJ9708011 induced time- and concentration-dependent G1 arrest of the cell cycle and subsequent apoptosis in human prostate cancer cells. The G1-arrest effect was confirmed by down-regulated expressions of several G1-phase regulators, including cyclin D1, cyclin E, cyclin-dependent kinase (Cdk)-4, Cdk2, phospho-RB, E2F-1, and Cdc25A. The mRNA expressions of cyclin D1 and cyclin E were also inhibited through the suppression of NF-κB. WJ9708011 blocked the protein synthesis and inhibited mammalian target of rapamycin (mTOR) signaling pathways. The suppression of mTOR pathways were irrespective of Akt- and AMPK-activated protein kinase (AMPK), but were attributed to mitochondrial stress, in which the down-regulation of survivin protein level may play a crucial role. CONCLUSIONS. The data suggest that WJ9708011 induces transcriptional and translational suppression of cell-cycle regulators that might be through Akt- and AMPK-independent loss of Δψm and inhibition of mTOR signaling pathway, leading to G1 arrest of the cell cycle and subsequent apoptotic cell death.

Original languageEnglish
Pages (from-to)1295-1306
Number of pages12
JournalProstate
Volume70
Issue number12
DOIs
Publication statusPublished - Sep 1 2010

Fingerprint

Sirolimus
Cell Cycle Checkpoints
Phosphatidylinositol 3-Kinases
Prostatic Neoplasms
AMP-Activated Protein Kinases
Hormones
Apoptosis
G1 Phase Cell Cycle Checkpoints
Cyclin E
Cyclin D1
Proteins
lissamine rhodamine B
Cell Cycle
Cyclin-Dependent Kinase 4
Chalcones
Messenger RNA
Mitochondrial Membrane Potential
G1 Phase
Leucine
Vegetables

Keywords

  • G1 arrest
  • Methoxychalcone
  • Mitochondrial stress
  • mTOR
  • Survivin

ASJC Scopus subject areas

  • Urology
  • Oncology

Cite this

Methoxychalcone induces cell-cycle arrest and apoptosis in human hormone-resistant prostate cancer cells through PI 3-kinase-independent inhibition of mTOR pathways. / Sun, Yu Wei; Huang, Wei Jan; Hsiao, Che Jen; Chen, Yi Cheng; Lu, Pin Hsuan; Guh, Jih Hwa.

In: Prostate, Vol. 70, No. 12, 01.09.2010, p. 1295-1306.

Research output: Contribution to journalArticle

Sun, Yu Wei ; Huang, Wei Jan ; Hsiao, Che Jen ; Chen, Yi Cheng ; Lu, Pin Hsuan ; Guh, Jih Hwa. / Methoxychalcone induces cell-cycle arrest and apoptosis in human hormone-resistant prostate cancer cells through PI 3-kinase-independent inhibition of mTOR pathways. In: Prostate. 2010 ; Vol. 70, No. 12. pp. 1295-1306.
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T1 - Methoxychalcone induces cell-cycle arrest and apoptosis in human hormone-resistant prostate cancer cells through PI 3-kinase-independent inhibition of mTOR pathways

AU - Sun, Yu Wei

AU - Huang, Wei Jan

AU - Hsiao, Che Jen

AU - Chen, Yi Cheng

AU - Lu, Pin Hsuan

AU - Guh, Jih Hwa

PY - 2010/9/1

Y1 - 2010/9/1

N2 - BACKGROUND. Chalcones are contained in fruits and vegetables, and have been suggested to display anticancer activities. In this study, the anticancer mechanism of WJ9708011 (a methoxychalcone derivative) was delineated in human prostate cancer cells. METHOD. Cell proliferation was examined by sulforhodamine B and clonogenic assays. Cell-cycle progression and mitochondrial membrane potential (Δψm) were detected by flow cytometric analysis. Expressions of protein and mRNA were detected by Western blot and RT-PCR technique, respectively. The protein synthesis was examined by [ 3H]leucine incorporation assay. The overexpression or knockdown techniques for specific target protein were also used in this study. RESULTS. WJ9708011 induced time- and concentration-dependent G1 arrest of the cell cycle and subsequent apoptosis in human prostate cancer cells. The G1-arrest effect was confirmed by down-regulated expressions of several G1-phase regulators, including cyclin D1, cyclin E, cyclin-dependent kinase (Cdk)-4, Cdk2, phospho-RB, E2F-1, and Cdc25A. The mRNA expressions of cyclin D1 and cyclin E were also inhibited through the suppression of NF-κB. WJ9708011 blocked the protein synthesis and inhibited mammalian target of rapamycin (mTOR) signaling pathways. The suppression of mTOR pathways were irrespective of Akt- and AMPK-activated protein kinase (AMPK), but were attributed to mitochondrial stress, in which the down-regulation of survivin protein level may play a crucial role. CONCLUSIONS. The data suggest that WJ9708011 induces transcriptional and translational suppression of cell-cycle regulators that might be through Akt- and AMPK-independent loss of Δψm and inhibition of mTOR signaling pathway, leading to G1 arrest of the cell cycle and subsequent apoptotic cell death.

AB - BACKGROUND. Chalcones are contained in fruits and vegetables, and have been suggested to display anticancer activities. In this study, the anticancer mechanism of WJ9708011 (a methoxychalcone derivative) was delineated in human prostate cancer cells. METHOD. Cell proliferation was examined by sulforhodamine B and clonogenic assays. Cell-cycle progression and mitochondrial membrane potential (Δψm) were detected by flow cytometric analysis. Expressions of protein and mRNA were detected by Western blot and RT-PCR technique, respectively. The protein synthesis was examined by [ 3H]leucine incorporation assay. The overexpression or knockdown techniques for specific target protein were also used in this study. RESULTS. WJ9708011 induced time- and concentration-dependent G1 arrest of the cell cycle and subsequent apoptosis in human prostate cancer cells. The G1-arrest effect was confirmed by down-regulated expressions of several G1-phase regulators, including cyclin D1, cyclin E, cyclin-dependent kinase (Cdk)-4, Cdk2, phospho-RB, E2F-1, and Cdc25A. The mRNA expressions of cyclin D1 and cyclin E were also inhibited through the suppression of NF-κB. WJ9708011 blocked the protein synthesis and inhibited mammalian target of rapamycin (mTOR) signaling pathways. The suppression of mTOR pathways were irrespective of Akt- and AMPK-activated protein kinase (AMPK), but were attributed to mitochondrial stress, in which the down-regulation of survivin protein level may play a crucial role. CONCLUSIONS. The data suggest that WJ9708011 induces transcriptional and translational suppression of cell-cycle regulators that might be through Akt- and AMPK-independent loss of Δψm and inhibition of mTOR signaling pathway, leading to G1 arrest of the cell cycle and subsequent apoptotic cell death.

KW - G1 arrest

KW - Methoxychalcone

KW - Mitochondrial stress

KW - mTOR

KW - Survivin

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