Method development for the determination of teicoplanin in patient serum by solid phase extraction and micellar electrokinetic chromatography

I. Lin Tsai, Fe Lin Lin Wu, Churn Shiouh Gau, Ching Hua Kuo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In-hospital deaths caused by the infection of methicillin-resistant Staphylococcus aureus (MRSA) are on the increase worldwide. Teicoplanin is a potent glycopeptide antibiotic against MRSA. A rapid and cost-saving micellar electrokinetic chromatography (MEKC) method combined with solid phase extraction (SPE) was developed and then validated to quantify teicoplanin in patient serum in this work. The method includes the following steps: (1) pretreatment of the serum samples with 10 M urea to denature proteins, (2) application of SPE by using an OASIS HLB cartridge to clean up and concentrate the serum samples, and (3) use of MEKC for sample analysis. Under the optimized conditions, the SPE recovery of teicoplanin is higher than 90%. The six major components of teicoplanin could be baseline-separated from one another and endogenous materials in 12 min with a background electrolyte composed of 20 mM sodium tetraborate buffer pH 8.8, 40 mM sodium dodecyl sulfate, and 11% (v/v) ACN. The relative standard deviation (R.S.D.) of the peak area ratios for method repeatability (n = 6) and intermediate precision (inter-day, n = 3) were found to be lower than 4.18% and 5.30%, respectively. The calibration curves were linear between the chromatographic response and total teicoplanin concentration over the range of 5 μg/mL to 55 μg/mL. Limit of detection (LOD) for each of the six components was found to be lower than 0.06 μg/mL. Pearson's correlation revealed that a good correlation (r = 0.98) was obtained between the SPE-MEKC method and the fluorescence polarization immunoassay (FPIA) method. The developed method can be used to quantitatively determine serum teicoplanin concentration in patients for dose monitoring and clinical research.

Original languageEnglish
Pages (from-to)1208-1216
Number of pages9
JournalTalanta
Volume77
Issue number3
DOIs
Publication statusPublished - Jan 15 2009
Externally publishedYes

Fingerprint

Teicoplanin
Solid Phase Extraction
Chromatography
Serum
Methicillin
Methicillin-Resistant Staphylococcus aureus
Fluorescence Polarization Immunoassay
Glycopeptides
Physiologic Monitoring
Sodium Dodecyl Sulfate
Electrolytes
Calibration
Urea
Limit of Detection
Buffers
Fluorescence
Polarization
Anti-Bacterial Agents
Costs and Cost Analysis
Recovery

Keywords

  • Micellar electrokinetic chromatography
  • Serum
  • Solid phase extraction
  • Teicoplanin

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Method development for the determination of teicoplanin in patient serum by solid phase extraction and micellar electrokinetic chromatography. / Tsai, I. Lin; Wu, Fe Lin Lin; Gau, Churn Shiouh; Kuo, Ching Hua.

In: Talanta, Vol. 77, No. 3, 15.01.2009, p. 1208-1216.

Research output: Contribution to journalArticle

@article{b89f5db059594718bca7bbe34cf6449f,
title = "Method development for the determination of teicoplanin in patient serum by solid phase extraction and micellar electrokinetic chromatography",
abstract = "In-hospital deaths caused by the infection of methicillin-resistant Staphylococcus aureus (MRSA) are on the increase worldwide. Teicoplanin is a potent glycopeptide antibiotic against MRSA. A rapid and cost-saving micellar electrokinetic chromatography (MEKC) method combined with solid phase extraction (SPE) was developed and then validated to quantify teicoplanin in patient serum in this work. The method includes the following steps: (1) pretreatment of the serum samples with 10 M urea to denature proteins, (2) application of SPE by using an OASIS HLB cartridge to clean up and concentrate the serum samples, and (3) use of MEKC for sample analysis. Under the optimized conditions, the SPE recovery of teicoplanin is higher than 90{\%}. The six major components of teicoplanin could be baseline-separated from one another and endogenous materials in 12 min with a background electrolyte composed of 20 mM sodium tetraborate buffer pH 8.8, 40 mM sodium dodecyl sulfate, and 11{\%} (v/v) ACN. The relative standard deviation (R.S.D.) of the peak area ratios for method repeatability (n = 6) and intermediate precision (inter-day, n = 3) were found to be lower than 4.18{\%} and 5.30{\%}, respectively. The calibration curves were linear between the chromatographic response and total teicoplanin concentration over the range of 5 μg/mL to 55 μg/mL. Limit of detection (LOD) for each of the six components was found to be lower than 0.06 μg/mL. Pearson's correlation revealed that a good correlation (r = 0.98) was obtained between the SPE-MEKC method and the fluorescence polarization immunoassay (FPIA) method. The developed method can be used to quantitatively determine serum teicoplanin concentration in patients for dose monitoring and clinical research.",
keywords = "Micellar electrokinetic chromatography, Serum, Solid phase extraction, Teicoplanin",
author = "Tsai, {I. Lin} and Wu, {Fe Lin Lin} and Gau, {Churn Shiouh} and Kuo, {Ching Hua}",
year = "2009",
month = "1",
day = "15",
doi = "10.1016/j.talanta.2008.08.022",
language = "English",
volume = "77",
pages = "1208--1216",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Method development for the determination of teicoplanin in patient serum by solid phase extraction and micellar electrokinetic chromatography

AU - Tsai, I. Lin

AU - Wu, Fe Lin Lin

AU - Gau, Churn Shiouh

AU - Kuo, Ching Hua

PY - 2009/1/15

Y1 - 2009/1/15

N2 - In-hospital deaths caused by the infection of methicillin-resistant Staphylococcus aureus (MRSA) are on the increase worldwide. Teicoplanin is a potent glycopeptide antibiotic against MRSA. A rapid and cost-saving micellar electrokinetic chromatography (MEKC) method combined with solid phase extraction (SPE) was developed and then validated to quantify teicoplanin in patient serum in this work. The method includes the following steps: (1) pretreatment of the serum samples with 10 M urea to denature proteins, (2) application of SPE by using an OASIS HLB cartridge to clean up and concentrate the serum samples, and (3) use of MEKC for sample analysis. Under the optimized conditions, the SPE recovery of teicoplanin is higher than 90%. The six major components of teicoplanin could be baseline-separated from one another and endogenous materials in 12 min with a background electrolyte composed of 20 mM sodium tetraborate buffer pH 8.8, 40 mM sodium dodecyl sulfate, and 11% (v/v) ACN. The relative standard deviation (R.S.D.) of the peak area ratios for method repeatability (n = 6) and intermediate precision (inter-day, n = 3) were found to be lower than 4.18% and 5.30%, respectively. The calibration curves were linear between the chromatographic response and total teicoplanin concentration over the range of 5 μg/mL to 55 μg/mL. Limit of detection (LOD) for each of the six components was found to be lower than 0.06 μg/mL. Pearson's correlation revealed that a good correlation (r = 0.98) was obtained between the SPE-MEKC method and the fluorescence polarization immunoassay (FPIA) method. The developed method can be used to quantitatively determine serum teicoplanin concentration in patients for dose monitoring and clinical research.

AB - In-hospital deaths caused by the infection of methicillin-resistant Staphylococcus aureus (MRSA) are on the increase worldwide. Teicoplanin is a potent glycopeptide antibiotic against MRSA. A rapid and cost-saving micellar electrokinetic chromatography (MEKC) method combined with solid phase extraction (SPE) was developed and then validated to quantify teicoplanin in patient serum in this work. The method includes the following steps: (1) pretreatment of the serum samples with 10 M urea to denature proteins, (2) application of SPE by using an OASIS HLB cartridge to clean up and concentrate the serum samples, and (3) use of MEKC for sample analysis. Under the optimized conditions, the SPE recovery of teicoplanin is higher than 90%. The six major components of teicoplanin could be baseline-separated from one another and endogenous materials in 12 min with a background electrolyte composed of 20 mM sodium tetraborate buffer pH 8.8, 40 mM sodium dodecyl sulfate, and 11% (v/v) ACN. The relative standard deviation (R.S.D.) of the peak area ratios for method repeatability (n = 6) and intermediate precision (inter-day, n = 3) were found to be lower than 4.18% and 5.30%, respectively. The calibration curves were linear between the chromatographic response and total teicoplanin concentration over the range of 5 μg/mL to 55 μg/mL. Limit of detection (LOD) for each of the six components was found to be lower than 0.06 μg/mL. Pearson's correlation revealed that a good correlation (r = 0.98) was obtained between the SPE-MEKC method and the fluorescence polarization immunoassay (FPIA) method. The developed method can be used to quantitatively determine serum teicoplanin concentration in patients for dose monitoring and clinical research.

KW - Micellar electrokinetic chromatography

KW - Serum

KW - Solid phase extraction

KW - Teicoplanin

UR - http://www.scopus.com/inward/record.url?scp=57049126503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57049126503&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2008.08.022

DO - 10.1016/j.talanta.2008.08.022

M3 - Article

VL - 77

SP - 1208

EP - 1216

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 3

ER -