Abstract

The potential effects of inhaled fine particulate matter (PM2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM2.5 from haze episodes on protein oxidation. PM2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM2.5 in BJ and XA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM2.5 increased oxidative stress. Addition of PM2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes.

Original languageEnglish
Pages (from-to)384-391
Number of pages8
JournalJournal of Hazardous Materials
Volume274
DOIs
Publication statusPublished - Jun 15 2014

Fingerprint

Particulate Matter
haze
Methionine
particulate matter
Albumins
Hong Kong
oxidation
Oxidation
Polycyclic Aromatic Hydrocarbons
Polycyclic aromatic hydrocarbons
Air Pollution
Bronchoalveolar Lavage Fluid
PAH
Nose
Proteins
Suspensions
Oxidative Stress
protein
Oxidative stress
Air pollution

Keywords

  • Air pollution
  • Liquid chromatography-mass spectrometry
  • Polycyclic aromatic hydrocarbons
  • Protein structure
  • Pulmonary

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering
  • Medicine(all)

Cite this

Methionine oxidation in albumin by fine haze particulate matter : An in vitro and in vivo study. / Lee, Kang Yun; Wong, Chris Kong Chu; Chuang, Kai Jen; Bien, Mauo Ying; Cao, Jun Ji; Han, Yong Ming; Tian, Linwei; Chang, Chih Cheng; Feng, Po Hao; Ho, Kin Fai; Chuang, Hsiao Chi.

In: Journal of Hazardous Materials, Vol. 274, 15.06.2014, p. 384-391.

Research output: Contribution to journalArticle

Lee, Kang Yun ; Wong, Chris Kong Chu ; Chuang, Kai Jen ; Bien, Mauo Ying ; Cao, Jun Ji ; Han, Yong Ming ; Tian, Linwei ; Chang, Chih Cheng ; Feng, Po Hao ; Ho, Kin Fai ; Chuang, Hsiao Chi. / Methionine oxidation in albumin by fine haze particulate matter : An in vitro and in vivo study. In: Journal of Hazardous Materials. 2014 ; Vol. 274. pp. 384-391.
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