Methanolic Extract of Rhizoma Coptidis Inhibits the Early Viral Entry Steps of Hepatitis C Virus Infection

Ting Chun Hung, Alagie Jassey, Chien Ju Lin, Ching Hsuan Liu, Chun Ching Lin, Ming Hong Yen, Liang Tzung Lin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Hepatitis C Virus (HCV) remains an important public health threat with approximately 170 million carriers worldwide who are at risk of developing hepatitis C-associated end-stage liver diseases. Despite improvement of HCV treatment using the novel direct-acting antivirals (DAAs) targeting viral replication, there is a lack of prophylactic measures for protection against HCV infection. Identifying novel antivirals such as those that target viral entry could help broaden the therapeutic arsenal against HCV. Herein, we investigated the anti-HCV activity of the methanolic extract from Rhizoma coptidis (RC), a widely used traditional Chinese medicine documented by the WHO and experimentally reported to possess several pharmacological functions including antiviral effects. Using the cell culture-derived HCV system, we demonstrated that RC dose-dependently inhibited HCV infection of Huh-7.5 cells at non-cytotoxic concentrations. In particular, RC blocked HCV attachment and entry/fusion into the host cells without exerting any significant effect on the cell-free viral particles or modulating key host cell entry factors to HCV. Moreover, RC robustly suppressed HCV pseudoparticles infection of Huh-7.5 cells and impeded infection by several HCV genotypes. Collectively, our results identified RC as a potent antagonist to HCV entry with potential pan-genotypic properties, which deserves further evaluation for use as an anti-HCV agent.

Original languageEnglish
JournalViruses
Volume10
Issue number12
DOIs
Publication statusPublished - Nov 27 2018

Fingerprint

Virus Diseases
Hepacivirus
Antiviral Agents
Virus Internalization
Coptidis rhizoma extract
Virus Attachment
End Stage Liver Disease
Chinese Traditional Medicine
Hepatitis C
Virion
Public Health
Cell Culture Techniques
Genotype
Pharmacology

Keywords

  • antiviral
  • entry inhibition
  • HCV
  • herbal medicine
  • Rhizoma coptidis

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Methanolic Extract of Rhizoma Coptidis Inhibits the Early Viral Entry Steps of Hepatitis C Virus Infection. / Hung, Ting Chun; Jassey, Alagie; Lin, Chien Ju; Liu, Ching Hsuan; Lin, Chun Ching; Yen, Ming Hong; Lin, Liang Tzung.

In: Viruses, Vol. 10, No. 12, 27.11.2018.

Research output: Contribution to journalArticle

Hung, Ting Chun ; Jassey, Alagie ; Lin, Chien Ju ; Liu, Ching Hsuan ; Lin, Chun Ching ; Yen, Ming Hong ; Lin, Liang Tzung. / Methanolic Extract of Rhizoma Coptidis Inhibits the Early Viral Entry Steps of Hepatitis C Virus Infection. In: Viruses. 2018 ; Vol. 10, No. 12.
@article{849db4e05fd24d3ca55f165b0981a960,
title = "Methanolic Extract of Rhizoma Coptidis Inhibits the Early Viral Entry Steps of Hepatitis C Virus Infection",
abstract = "Hepatitis C Virus (HCV) remains an important public health threat with approximately 170 million carriers worldwide who are at risk of developing hepatitis C-associated end-stage liver diseases. Despite improvement of HCV treatment using the novel direct-acting antivirals (DAAs) targeting viral replication, there is a lack of prophylactic measures for protection against HCV infection. Identifying novel antivirals such as those that target viral entry could help broaden the therapeutic arsenal against HCV. Herein, we investigated the anti-HCV activity of the methanolic extract from Rhizoma coptidis (RC), a widely used traditional Chinese medicine documented by the WHO and experimentally reported to possess several pharmacological functions including antiviral effects. Using the cell culture-derived HCV system, we demonstrated that RC dose-dependently inhibited HCV infection of Huh-7.5 cells at non-cytotoxic concentrations. In particular, RC blocked HCV attachment and entry/fusion into the host cells without exerting any significant effect on the cell-free viral particles or modulating key host cell entry factors to HCV. Moreover, RC robustly suppressed HCV pseudoparticles infection of Huh-7.5 cells and impeded infection by several HCV genotypes. Collectively, our results identified RC as a potent antagonist to HCV entry with potential pan-genotypic properties, which deserves further evaluation for use as an anti-HCV agent.",
keywords = "antiviral, entry inhibition, HCV, herbal medicine, Rhizoma coptidis",
author = "Hung, {Ting Chun} and Alagie Jassey and Lin, {Chien Ju} and Liu, {Ching Hsuan} and Lin, {Chun Ching} and Yen, {Ming Hong} and Lin, {Liang Tzung}",
year = "2018",
month = "11",
day = "27",
doi = "10.3390/v10120669",
language = "English",
volume = "10",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",

}

TY - JOUR

T1 - Methanolic Extract of Rhizoma Coptidis Inhibits the Early Viral Entry Steps of Hepatitis C Virus Infection

AU - Hung, Ting Chun

AU - Jassey, Alagie

AU - Lin, Chien Ju

AU - Liu, Ching Hsuan

AU - Lin, Chun Ching

AU - Yen, Ming Hong

AU - Lin, Liang Tzung

PY - 2018/11/27

Y1 - 2018/11/27

N2 - Hepatitis C Virus (HCV) remains an important public health threat with approximately 170 million carriers worldwide who are at risk of developing hepatitis C-associated end-stage liver diseases. Despite improvement of HCV treatment using the novel direct-acting antivirals (DAAs) targeting viral replication, there is a lack of prophylactic measures for protection against HCV infection. Identifying novel antivirals such as those that target viral entry could help broaden the therapeutic arsenal against HCV. Herein, we investigated the anti-HCV activity of the methanolic extract from Rhizoma coptidis (RC), a widely used traditional Chinese medicine documented by the WHO and experimentally reported to possess several pharmacological functions including antiviral effects. Using the cell culture-derived HCV system, we demonstrated that RC dose-dependently inhibited HCV infection of Huh-7.5 cells at non-cytotoxic concentrations. In particular, RC blocked HCV attachment and entry/fusion into the host cells without exerting any significant effect on the cell-free viral particles or modulating key host cell entry factors to HCV. Moreover, RC robustly suppressed HCV pseudoparticles infection of Huh-7.5 cells and impeded infection by several HCV genotypes. Collectively, our results identified RC as a potent antagonist to HCV entry with potential pan-genotypic properties, which deserves further evaluation for use as an anti-HCV agent.

AB - Hepatitis C Virus (HCV) remains an important public health threat with approximately 170 million carriers worldwide who are at risk of developing hepatitis C-associated end-stage liver diseases. Despite improvement of HCV treatment using the novel direct-acting antivirals (DAAs) targeting viral replication, there is a lack of prophylactic measures for protection against HCV infection. Identifying novel antivirals such as those that target viral entry could help broaden the therapeutic arsenal against HCV. Herein, we investigated the anti-HCV activity of the methanolic extract from Rhizoma coptidis (RC), a widely used traditional Chinese medicine documented by the WHO and experimentally reported to possess several pharmacological functions including antiviral effects. Using the cell culture-derived HCV system, we demonstrated that RC dose-dependently inhibited HCV infection of Huh-7.5 cells at non-cytotoxic concentrations. In particular, RC blocked HCV attachment and entry/fusion into the host cells without exerting any significant effect on the cell-free viral particles or modulating key host cell entry factors to HCV. Moreover, RC robustly suppressed HCV pseudoparticles infection of Huh-7.5 cells and impeded infection by several HCV genotypes. Collectively, our results identified RC as a potent antagonist to HCV entry with potential pan-genotypic properties, which deserves further evaluation for use as an anti-HCV agent.

KW - antiviral

KW - entry inhibition

KW - HCV

KW - herbal medicine

KW - Rhizoma coptidis

UR - http://www.scopus.com/inward/record.url?scp=85057562417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057562417&partnerID=8YFLogxK

U2 - 10.3390/v10120669

DO - 10.3390/v10120669

M3 - Article

VL - 10

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 12

ER -